Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma

Abstract

In light of PRP’s increasing popularity in veterinary practice, this study aimed to compare three manual methods to prepare and cool equine PRP. The blood of 18 clinically healthy mares was collected via venipuncture in a blood transfusion bag (method 1), blood tubes (method 2), and a syringe (method 3). In method 1, samples were double centrifuged; method 2 involved one centrifugation, and in method 3 the syringe was kept in an upright position to sediment for 4 h. After processing with three methods, PRP and platelet-poor plasma (PPP) were extracted and assessed for red (RBC) and white blood cell counts (WBC), platelet counts, and viability. In a subset of mares (n = 6), samples were processed with the three methods, and PRP was evaluated at 6 and 24 h postcooling at 5 °C. Method 1 resulted in the highest and method 3 in the lowest platelet concentration (p < 0.05), and the latter also had greater contamination with WBC than the others (p < 0.001). Platelet viability was similar across treatments (p > 0.05). Cooling for 24 h did not affect platelet counts in all methods (p > 0.05); however, platelet viability was reduced after cooling PRP produced by method 3 (p = 0.04), and agglutination increased over time in all methods (p < 0.001). The three methods increased (1.8–5.6-fold) platelet concentration in PRP compared to whole blood without compromising platelet viability. In conclusion, all three methods concentrated platelets and while cooling affected their viability. It remains unknown whether the different methods and cooling would affect PRP’s clinical efficacy.

A one-health perspective: use of hemoderivative regenerative therapies in canine and equine patients

“…Simple manual centrifugation techniques have been described. 42,43 A filtration technique that separates platelets from whole blood has been validated for use in horses and dogs. 36,44 When choosing a processing system, determine that it has been validated for the intended species, it is compatible with the location of use, and the system manufacturer/distributor provides support as needed.…”

General and comparative aspects of endometritis in domestic species: A review

“…Recently, plasma products have therefore gained increasing attention for the treatment of PBIE because such substances can be produced noncommercially. Among those, platelet‐rich plasma has become quite popular (Segabinazzi, Canisso, et al 2021). It includes various growth factors that may help to reduce endometritis in mares with PBIE (Reghini et al, 2016) and thus improve fertility (Segabinazzi, Podico, et al, 2021).…”

Simple Tube Centrifugation Method for Platelet-Rich Plasma (PRP) Preparation in Catalonian Donkeys as a Treatment of Endometritis-Endometrosis

“…Because there are many different protocols used both in the human and veterinary literature and a lack of standardization in the realm of publications, the efficacy and treatment results are difficult to compare. Recently, Segabinazzi et al [16] compared three different protocols to prepare equine PRP, obtaining no significant differences between one and two centrifugations, but did obtain a reduced platelet recovery using simple sedimentation. In the field of equine veterinary medicine, PRP is commonly used to treat degenerative joint diseases [17,18], tendinitis [19], ligamentous lesions [20,21], and persistent mating-induced endometritis (PMIE) [16,22,23].…”