Three New Lipases from Actinomycetes and Their Use in Organic Reactions

Cardenas, F.; Álvarez, Emilio; Castro-Alvarez, M. S. De; Sánchez-Montero, J.M.; Elson, Stephen W.; Sinisterra, J. V.

doi:10.3109/10242420109003647

Cited by 8 publications

(7 citation statements)

References 19 publications

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“…This profile is similar to those described for other lipase-producing actinomycetes. However, the time course for optimum lipase production (96 h) in the present study was less than other lipase-producing actinomycetes reported [7]. The decrease in lipase production after long fermentation times could be due to inactivation of the enzyme by extracellular proteases, as observed for other lipase-producing microorganisms [56].…”

Section: Discussioncontrasting

confidence: 58%

“…In some cases, lipases were produced constitutively and showed significant increase in activity on addition of oil to the medium [49]. Similarly, lipase induction in presence of lipid-based carbon sources was reported in actinomycetes [7,31]. Macris et al suggested that it was the carbon chain moiety of the fatty acid present in the triacylglycerol that controlled lipase synthesis [38].…”

Section: Discussionmentioning

confidence: 91%

“…4). Most of the actinomycetes reported for lipase production were grown at neutral initial pH for optimal growth and lipase production [7,31]. However, maximum lipase activity at higher initial pH by various thermophilic Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

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Influence of cultivation conditions on the production of a thermostable extracellular lipase from Amycolatopsis mediterranei DSM 43304

Dheeman¹,

Frias²,

Henehan³

2009

J Ind Microbiol Biotechnol

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23Among several lipase producing actinomycete strains screened, Amycolatopsis 24 mediterranei DSM 43304 was found to produce a thermostable, extracellular lipase. 25Culture condition and nutrient source modification studies involving carbon sources, 26 nitrogen sources, incubation temperature and medium pH were carried out. Lipase activity 27 of 1.37 ± 0.103 IU/ml of culture medium was obtained in 96 h at 28 °C and pH 7.5 using 28 linseed oil and fructose as carbon sources and a combination of phytone peptone and yeast 29 extract (5:1) as nitrogen sources. In optimal culture conditions the lipase activity was 30 enhanced 12-fold with a 2-fold increase in lipase specific activity. The lipase showed 31 maximum activity at 60 °C and pH 8.

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Section: Discussioncontrasting

confidence: 58%

Section: Discussionmentioning

confidence: 91%

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Influence of cultivation conditions on the production of a thermostable extracellular lipase from Amycolatopsis mediterranei DSM 43304

Dheeman¹,

Frias²,

Henehan³

2009

J Ind Microbiol Biotechnol

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“…In sucrose variation, the mycelial growth (32.0 mg dry weight) and the production (50.0 U/ml) were maximum at 1.5 and 2.0 g/l concentration respectively. In an earlier report, lipase production from Amycolatopsis mediterranei DSM 43304 was influenced by the amendment of lactose in the medium 33 ; similarly lipase induction in the presence of sugar sources were optimum in the actinomycetes 34 .…”

Section: Carbon Sourcesmentioning

confidence: 85%

Untitled

2013

IJPSR

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Partial purification and bioremediation of waste water by lipase from the marine actinomycete Streptomyces variabilis NGP 3 (Accession no: (JX843530)) were carried out. The optimum incubation period, pH, temperature and agitation speed for enzyme production were fifth day (61.2 U/ml), 9.0 -9.5 (105 U/ml), 35ºC (39.4 U/ml) and 120 rpm (38.7 U/ml) respectively. Lactose (2.0 g/l) and peptone (0.6 and 0.8 g/l) proved to the best carbon and nitrogen sources respectively for lipase production. The partially purified lipase showed a specific activity of 1440.97 U/mg protein, 7.63 fold pure and yielded 3.19 per cent of protein. The enzyme activity was maximum at the pH and temperatures were 8.5 and 45ºC respectively. The molecular weight of the first and second isoenzymes was found to be 55.0 and 56.0 KDa respectively. Bioremediation of automobile effluent and slaughter house waste water were carried out by the isolated actinomycetes isolate S. variabilis NGP 3. The chemical oxygen demand (COD), total organic chloride (TOC) and fat/oil content of the effluent were analyzed. The COD and fat/oil degradation rate were increased by the simultaneous reduction of TOC in the treated effluent.

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“…One isolate, B12 was positive for the lipase activity.However, the literature suggests actinomycetes as a good source of lipase (Cardenas et al, 2001). This low estimate could be due to the error inthe methoddescribed in (Sierra, 1957) used for preliminary screening and testing or maybe low availability of lipids in the sampling environment.…”

Section: Enzymatic Activity Of Soil Bacteriamentioning

confidence: 99%