Three new steroidal saponins from Aspidistra letreae plants and their cytotoxic activities

Ho, Duc Viet; Hoang, Hanh Nhu Thi; Vo, Hung; Nguyen, Khan Viet; Pham, Ty Viet; Le, Anh‐Tuan; Phan, Kiem Van; Nguyen, Hien Minh; Morita, Hiroyuki; Nguyen, Hoai Thi

doi:10.1007/s11418-020-01395-9

Cited by 18 publications

(25 citation statements)

References 22 publications

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“…The selectivity index calculated as the IC 50 of normal cells/IC 50 of cancer cells, demonstrated that AA has a selectivity index in the range of 1.7-2.9, indicating that this compound preferentially induced cytotoxicity in lung cancer. Generally, the present results are in good agreement with our previous study [25].…”

Section: Cytotoxicity Of Aa On H460 H23 and A549 Human Lung Cancer Csupporting

confidence: 94%

“…These properties are mainly attributed to the presence of polysaccharides and their derivatives [30,31]. Previous studies reported that AA may have potential activities against cancers [25]. However, the anticancer activities of AA with respect to cancer cell proliferation and metastasis have not yet been elucidated.…”

Section: Discussionmentioning

confidence: 99%

“…Aspiletrein A (AA), a dry powder, was isolated and characterized as previously described [25]. The structure was determined by NMR analysis on a Bruker Avance 500 spectrometer (Bruker, MA, USA), and the HRESIMS was recorded on an Agilent 6545 accurate-mass spectrometer (Agilent, CA, USA [25]. AA was dissolved in DMSO to yield a stock solution and further diluted in complete medium to the desired concentrations before use.…”

Section: Test Compound and Reagentsmentioning

confidence: 99%

“…A previous study demonstrated that AA exhibited the strongest cytotoxicity among isolated saponins and exerted potent effects against the LU-1 cell line, with an IC 50 value of 9.94 ± 2.15 μM [25]. The cytotoxic effect was extensively investigated in H460, H23 and A549 non-small cell lung cancer cells by MTT assay.…”

Section: Cytotoxicity Of Aa On H460 H23 and A549 Human Lung Cancer Cmentioning

confidence: 99%

“…Previously, phytochemical investigations of active steroidal saponins led to their isolation and characterization, and aspiletrein A (AA) ( Fig. 1) showed the greatest cytotoxicity against five human cancer cell lines, including lung adenocarcinoma (LU-1), cervical carcinoma (HeLa), breast adenocarcinoma (MDA-MB-231), liver hepatocellular carcinoma (HepG2) and gastric adenocarcinoma (MKN-7) cell lines, with IC 50 values less than 12.5 μM [25]. In the present study, we continued investigating the in vitro anticancer activities, particularly the antiproliferation, antimigration and anti-invasion activities, of AA in H460, H23 and A549 lung cancer cells.…”

Section: Introductionmentioning

confidence: 99%

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Antitumor activities of Aspiletrein A, a steroidal saponin from Aspidistra letreae, on non-small cell lung cancer cells

Nguyen

Seephan

et al. 2021

BMC Complement Med Ther

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Background Lung cancer is one of the leading causes of death worldwide due to its strong proliferative and metastatic capabilities. The suppression of these aggressive behaviors is of interest in anticancer drug research and discovery. In recent years, many plants have been explored in order to discover new bioactive secondary metabolites to treat cancers or enhance treatment efficiency. Aspiletrein A (AA) is a steroidal saponin isolated from the whole endemic species Aspidistra letreae in Vietnam. Previously, elucidation of the structure of AA and screening of its cytotoxic activity against several cancer cell lines were reported. However, the antitumor activities and mechanisms of action have not yet been elucidated. In this study, we demonstrated the anti-proliferative, anti-migrative and anti-invasive effects of AA on H460, H23 and A549 human lung cancer cells. Methods MTT, wound healing and Transwell invasion assays were used to evaluate the anti-proliferation, anti-migration and anti-invasion effects of AA, respectively. Moreover, the inhibitory effect of AA on the activity of protein kinase B (Akt), a central mediator of cancer properties, and apoptotic regulators in the Bcl-2 family proteins were investigated by Western blotting. Results AA exhibits antimetastatic effects in human lung cancer cells through the inhibition of the pAkt/Akt signaling pathway, which in turn resulted in a significant inhibitory effect of AA on the migration and invasion of the examined lung cancer cells. Conclusions Aspiletrein A may be a potent inhibitor of protein kinase B (Akt). Hence, AA could be further explored as a potential antimetastatic lead compound.

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Section: Cytotoxicity Of Aa On H460 H23 and A549 Human Lung Cancer Csupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Test Compound and Reagentsmentioning

confidence: 99%

Section: Cytotoxicity Of Aa On H460 H23 and A549 Human Lung Cancer Cmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Antitumor activities of Aspiletrein A, a steroidal saponin from Aspidistra letreae, on non-small cell lung cancer cells

Nguyen

Seephan

et al. 2021

BMC Complement Med Ther

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Antioxidant Activity of a New Xanthone Derivative from Aspidistra Letreae: In Vitro and In Silico Studies

Nguyen

et al. 2021

Chemistry & Biodiversity

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A new xanthone derivative, aspidxanthone A (1), and three known compounds ((2S)‐1‐(β‐D‐galactopyranosyloxy)‐3‐(hexadecanoyloxy)propan‐2‐yl (9Z,12Z)‐octadeca‐9,12‐dienoate (2), (25S)‐spirostane‐1β,3α,5β‐triol (3), and asparenyldiol (4)) were isolated from the whole of the endemic species Aspidistra letreae in Vietnam. Their structures were elucidated by means of extensive spectroscopic analyses and comparison with published data. In this study, we report the isolation and structure elucidation of a new compound aspidxanthone A, antioxidant activities of the extract and isolates 1–4, and in silico molecular docking of aspidxanthone A. The ethyl acetate extract had good antioxidant activity with an IC50 value of 26.3 μg mL−1. Among the isolates, aspidxanthone A exhibited DPPH reduction activity with an IC50 value of 11.2 μM, which is in the same range as that of the positive control, ascorbic acid. The mechanism of action of aspidxanthone A on the tyrosinase and xanthine oxidase proteins have been clarified by in silico studies.

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Comprehensive review of Bcl‐2 family proteins in cancer apoptosis: Therapeutic strategies and promising updates of natural bioactive compounds and small molecules

Iksen,

Witayateeraporn,

Hardianti

et al. 2024

Phytotherapy Research

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Cancer has a considerably higher fatality rate than other diseases globally and is one of the most lethal and profoundly disruptive ailments. The increasing incidence of cancer among humans is one of the greatest challenges in the field of healthcare. A significant factor in the initiation and progression of tumorigenesis is the dysregulation of physiological processes governing cell death, which results in the survival of cancerous cells. B‐cell lymphoma 2 (Bcl‐2) family members play important roles in several cancer‐related processes. Drug research and development have identified various promising natural compounds that demonstrate potent anticancer effects by specifically targeting Bcl‐2 family proteins and their associated signaling pathways. This comprehensive review highlights the substantial roles of Bcl‐2 family proteins in regulating apoptosis, including the intricate signaling pathways governing the activity of these proteins, the impact of reactive oxygen species, and the crucial involvement of proteasome degradation and the stress response. Furthermore, this review discusses advances in the exploration and potential therapeutic applications of natural compounds and small molecules targeting Bcl‐2 family proteins and thus provides substantial scientific information and therapeutic strategies for cancer management.

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Three new steroidal saponins from Aspidistra letreae plants and their cytotoxic activities

Cited by 18 publications

References 22 publications

Antitumor activities of Aspiletrein A, a steroidal saponin from Aspidistra letreae, on non-small cell lung cancer cells

Antitumor activities of Aspiletrein A, a steroidal saponin from Aspidistra letreae, on non-small cell lung cancer cells

Antioxidant Activity of a New Xanthone Derivative from Aspidistra Letreae: In Vitro and In Silico Studies

Comprehensive review of Bcl‐2 family proteins in cancer apoptosis: Therapeutic strategies and promising updates of natural bioactive compounds and small molecules

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