Three-photon fluorescence imaging of melanin with a dual-wedge confocal scanning system

Mega, Yair; Kerimo, Josef; Robinson, Joseph P.; Vakili, Ali; Johnson, Nicolette; DiMarzio, Charles A.

doi:10.1117/12.909534

Cited by 8 publications

(4 citation statements)

References 14 publications

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“…Melanin is the pigment inside human and animal skin. The SMPAF of melanin is a non-linear process in which the fluorescence of the specimen is excited at longer wavelength and detected at shorter wavelength [17][18][19][20][21][22]. However, unlike the commonly known multi-photon fluorescence, which requires a femtosecond pulsed laser as the excitation source, the SMPAF of melanin can be excited by a continuous wave (CW) laser after been activated by a near-infrared laser [23,24].…”

Section: Introductionmentioning

confidence: 98%

A novel multimodal laser scanning microscope control system

Lai

Gu²,

Karasek³

et al. 2015

SPIE Proceedings

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Traditional laser scanning microscopes require complex control systems to synchronize and control image acquisition. The control system is especially cumbersome in the multimodal laser scanning microscope. We have developed a novel multimodal laser scanning microscope control system based on a National Instruments multifunction data acquisition device (DAQ), which serves as both a data acquisition device and a programmable signal generator. The novel control system is low-cost and easy-to-build, with all components off-the-shelf. We have applied the control system in a multimodal laser scanning microscope. The control system has not only significantly decreased the complexity of the microscope, but also increased the system flexibility. We have demonstrated that the system can be easily customized for various applications.

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Section: Introductionmentioning

confidence: 98%

A novel multimodal laser scanning microscope control system

Lai

Gu²,

Karasek³

et al. 2015

SPIE Proceedings

Self Cite

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“…Some on-axis approaches, such as acousto-optic deflectors [4][5][6][7] and electro-optic deflectors [8][9][10][11][12], are highly expensive. A relatively low cost on-axis solution is the Risley Prisms based system [13][14][15], which creates complicated scanning patterns but lack the ability of doing a raster scan.…”

Section: Introductionmentioning

confidence: 99%

Customized profile lens based linear response fast spinning on-axis optical scanners for 3D laser scanning microscopy

et al. 2014

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Previously, we presented an on-axis linear-response linear-motion optical scanner. While the linear design is highly desired for engineering consideration, it was still lacking the scanning speed required for imaging applications. We here present a customized profile lens (CPL), tailored for high speed performance while maintaining the advantages of a linear response on-axis optical scanner. The device was built and tested experimentally on an optical bench. The test results demonstrate precise linear response and fast scanning speed, and revealed video frame rate scanning ability. The implementation of the CPLs in laser scanning systems is promising in improving the current 3D laser scanning microscopy systems by reducing the size, error, and complexity of the system, as well as other systems unitizing high speed laser scanning technique.

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“…In a Risley scanner light is transmitted through two prisms, and one or both prisms are rotated to change the angle of the scanned beam which produces a complex scan pattern that makes uniform beam scanning difficult. [10][11][12] Electro-optic (EO) and acousto-optic (AO) beam scanners can achieve higher scan rates than inertia-limited mechanical scanners and can maintain moderate to high efficiency of the scanned beam, but the scan resolution (number of resolvable spots) suffers. 13 Electrowetting on dielectric (EWOD) scanners provide another alternative to mechanical beam scanning that can achieve high beam scan speeds in transmissive layout.…”

Section: Introductionmentioning

confidence: 99%

Polarization-based, transmissive-reflective beam scanner operating around 1550 nm using off-the-shelf components

Baker

Lang²,

Dickensheets³

et al. 2023

Photonic Instrumentation Engineering X

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A novel transmissive-reflective beam scanner operating around 1550 nm wavelength has been demonstrated using two commercial wire grid polarizers (WGPs) with transmission axes offset by 45°, surrounding a latching garnet 45° Faraday rotator. Light in the system undergoes three 45° polarization rotations, resulting in two reflections inside the cavity between the two WGPs before being transmitted by the rear WGP. The two reflections inside the device facilitate beam steering in a transmissive layout (output beam emerging from the opposite side of the input beam). Polarization-resolved individual-component characterizations were performed for several WGPs and for the Faraday rotator to determine optical properties such as transmission efficiency and the polarization purity of the transmitted or reflected beams as a function of incidence angle to the optic, for multiple incident linear polarization states. This allowed us to both select the WGPs best suited to act as polarizing beam splitters in the system and to use the angle-resolved component data to predict the total transmission efficiency of the beam scanner as a function of front polarizer tilt angle (which in our geometry is half of the beam deflection angle). We then compared the predicted scanner efficiency with the measured transmission efficiency of the prototype beam scanner. The scanner maintained a transmission efficiency over 70% for beam deflection angles between ±45° (a 90° symmetric beam scan area) for a vertically oriented incident linear polarization state.

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Three-photon fluorescence imaging of melanin with a dual-wedge confocal scanning system

Cited by 8 publications

References 14 publications

A novel multimodal laser scanning microscope control system

A novel multimodal laser scanning microscope control system

Customized profile lens based linear response fast spinning on-axis optical scanners for 3D laser scanning microscopy

Polarization-based, transmissive-reflective beam scanner operating around 1550 nm using off-the-shelf components

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