Three simple ways to detect antibody—Antigen complex on flat surfaces

Adams, Ann L.; Klings, Madeleine; Fischer, Gena; Vroman, Leo

doi:10.1016/0022-1759(73)90018-5

Cited by 31 publications

(2 citation statements)

References 4 publications

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“…Strep B OIA (Biostar, Boulder, CO), a new optical immunoassay, is available for use for the rapid detection of GBS. The test is based on the principle of optical immuno-assay technology [3,4] and involves extraction of a carbohydrate antigen unique to GBS. When the antigen is placed on a test surface coated with antigroup B antibody, binding occurs between the antigen and the antibody.…”

Section: Introductionmentioning

confidence: 99%

Detection of group B streptococcus: Comparison of an optical immunoassay with direct plating and broth-enhanced culture methods

Nguyen

Gauthier

Myles

et al. 1998

J. Matern. Fetal Med.

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The aim of this study was to compare the diagnostic accuracy of an optical immunoassay (STREP B OIA, Biostar) to direct plating and broth‐enhanced culture for the detection of group B streptococcus (GBS) colonization of the lower genital tract in pregnant women. GBS cultures from the lower genital tract were obtained in a prospective fashion using a dual swab transport system from patients with risk factors for perinatal GBS infection. One swab was used to inoculate a trypticase soy agar plate with 5% sheep blood (TSA) and then placed in Lim broth. The other swab was used to perform the Strep B OIA. Growth of GBS by either direct plating or broth‐enhanced culture was used as the gold standard for determining GBS colonization. Of the 524 women in the study, 90 women had positive cultures (either TSA or Lim broth). The sensitivity, specificity, positive predictive value, and negative predictive value of the Strep B OIA were 47% (42/90), 96% (416/434), 70% (42/60), 90% (416/464). The sensitivity, specificity, positive predictive value, and negative predictive value of the TSA were 61% (55/90), 100% (434/434), 100% (55/55), 93% (434/469). The sensitivity, specificity, positive predictive value, and negative predictive value of Lim broth were 97% (87/90), 100% (434/434), 100% (87/87), and 97% (434/437). The sensitivity of the Strep B OIA to detect light GBS colonization and heavy GBS colonization, as determined by the TSA, was 53% (19/36) and 90% (17/19), respectively. The Strep B OIA and direct agar plate culture appear to be of limited clinical value due to their poor sensitivities. This study also demonstrates the need to use a selective medium such as Lim broth when assessing for GBS colonization of the lower genital tract. J. Matern.‐Fetal Med. 7:172–176, 1998. © 1998 Wiley‐Liss, Inc.

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Section: Introductionmentioning

confidence: 99%

Detection of group B streptococcus: Comparison of an optical immunoassay with direct plating and broth-enhanced culture methods

Nguyen

Gauthier

Myles

et al. 1998

J. Matern. Fetal Med.

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“…It addresses the same approach utilized by Adams et al [5] in 1973, where single known proteins were analysed directly on reflective surfaces based upon interference colour, stainability, and wettability. The immunospecific imaging method, presented here, generates more precise and sensitive information without regard to any of those requirements.…”

Section: Introductionmentioning

confidence: 99%

Direct immunodetection of surface adsorbed proteins

Warkentin

Lundström

Tengvall

1993

J Mater Sci: Mater Med

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A modification of standard immunoblotting techniques gives rise to a novel method of imaging surface associated proteins without removing them from their original arrangement on the surface. The original proteins and subsequent antibody probe reactions are performed directly on the surface. The substrate is adsorbed into the nitrocellulose support which, in semi-dry contact, reacts only in the regions in which the target protein is antigenically present. The sensitivity of the method is comparable to other immunological techniques and diffusion of the resulting images is negligible resulting in reproducible reblots from the same surface with high lateral resolution. The method can be applied to a wide variety of reasonably flat surfaces. It demonstrates two dimensional protein patterns as a function of varying surface characteristics as well as differing protein detectabilities in their purified form as opposed to the same molecule as a plasma protein component adsorbed onto a surface. The method promises to be a useful tool in circumstances where surface bound proteins are not completely removable from surfaces or are lost in commonly used extraction or analytical techniques.

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Covalent coupling of polysaccharides to silicon and silicon rubber surfaces

Elam

Nygren

Stenberg

1984

J. Biomed. Mater. Res.

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A method to produce a hydrogel by covalently coupling dextran polysaccharide to silicon and silicon rubber surfaces is described. A bifunctional epoxysilane was used as coupling mediator and the conditions for the reaction were optimized. Coupling of the silane to silicon rubber was possible only after oxidation of the surface by plasma etching. The reaction between the epoxy-group and dextran was concentration dependent and required high concentrations of dextran. The turnover rate of fibrinogen in solution at the hydrophilic surface was found to be high when measured by ellipsometry.

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Three simple ways to detect antibody—Antigen complex on flat surfaces

Cited by 31 publications

References 4 publications

Detection of group B streptococcus: Comparison of an optical immunoassay with direct plating and broth-enhanced culture methods

Detection of group B streptococcus: Comparison of an optical immunoassay with direct plating and broth-enhanced culture methods

Direct immunodetection of surface adsorbed proteins

Covalent coupling of polysaccharides to silicon and silicon rubber surfaces

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