Thrombocytosis induced in mice after subacute and subchronic V2O5 inhalation

González-Villalva, Adriana; Fortoul, Teresa I.; Avila-Costa, Marı́a Rosa; Piñón-Zárate, Gabriela; Rodríguez-Lara, Vianey; Martínez-Levy, Gabriela Ariadna; Rojas-Lemus, Marcela; Bizarro-Nevarez, Patricia; Díaz-Bech, Patricia; Mussali-Galante, Patricia; Colı́n-Barenque, Laura

doi:10.1191/0748233706th250oa

Cited by 33 publications

(25 citation statements)

References 10 publications

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“…Previous reports from our group noted that vanadium can affect other tissues as well, including the blood and nervous systems (Gonzalez-Villalva et al, 2006) (Avila-Costa et al, 2004). We also have reported that vanadium impairs the immune system, with changes induced in the morphology of the spleen and decreases in humoral immune responses (Piñon-Zarate et al, 2008).…”

Section: Introductionmentioning

confidence: 70%

“…We have previously reported that the immune system is compromised in a V-exposed mouse model; in those studies, morphological and functional changes were noted in spleens of mice exposed to V (Fortoul et al, 2002;Gonzalez-Villalva et al, 2006). Because of the critical participation of the spleen in immune responses/ system function, we wondered whether effects would also manifest in another key immune system organ, the thymus.…”

Section: Discussionmentioning

confidence: 99%

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CD11c decrease in mouse thymic dendritic cells after vanadium inhalation

Ustarroz-Cano¹,

García-Peláez²,

Piñón-Zárate³

et al. 2012

Journal of Immunotoxicology

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Section: Introductionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

CD11c decrease in mouse thymic dendritic cells after vanadium inhalation

Ustarroz-Cano¹,

García-Peláez²,

Piñón-Zárate³

et al. 2012

Journal of Immunotoxicology

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“…Although there are slight differences between those studies probably related to species differences or method of administering the vanadium, the tendency is to suppress the immune response. We have previously shown that the chronic inhalation of V 2 O 5 in CD1 mice induces an increase in the number and size of platelets (Gonzalez-Villalva et al, 2006). We have also seen that these vanadium-treated mice show macroscopic splenic lesions that might lead to histologic and functional alterations.…”

Section: Introductionmentioning

confidence: 80%

Vanadium Pentoxide Inhalation Provokes Germinal Center Hyperplasia and Suppressed Humoral Immune Responses

Piñón-Zárate

Rodríguez-Lara

Rojas-Lemus

et al. 2008

Journal of Immunotoxicology

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Vanadium, an important air pollutant derived from fuel product combustion, aggravates respiratory diseases and impairs cardiovascular function. In contrast, its effects on immune response are conflicting. The aim of our work was to determine if spleens of vanadium-exposed CD1 mice showed histological lesions that might result in immune response malfunction. One hundred and twelve CD-1 male mice were placed in an acrylic box and inhaled 0.02 M vanadium pentoxide (V 2 O 5 ); actual concentration in chamber ≈1.4 mg V 2 O 5 /m 3 ) for 1 hr/d, twice a week, for 12 wk. Control mice inhaled only vehicle. Eight mice were sacrificed prior to the exposures. Eight control and eight V 2 O 5 -exposed mice were sacrificed 24 hr after the second exposure of each week until the 12-wk study was over. Another 8 mice that completed the 12-wk regimen were immunized with recombinant Hepatitis B surface antigen (HBsAg; three times over an 8-wk period) before sacrifice and analyses of their levels of anti-HBsAg antibody (HBSAb) using ELISA. In all studies, at sacrifice, blood samples were obtained by direct heart puncture and the spleen was removed, weighed and processed for H-E staining and quantitation of CD19 cells. The results indicated that the spleen weight of V 2 O 5 -exposed animals peaked at 9 wk (546 ± 45 vs. 274 ± 27 mg, p < 0.0001) and thereafter progressively decreased (321 ± 39 mg at 12 wk, p < 0.001; control spleen = 298 ± 35 mg). Spleens of V 2 O 5 -exposed animals showed an increased number of very large and non-clearly delimited germinal centers (that contained more lymphocytes and megakaryocytes) compared to those of control mice. In addition, their red pulp was poorly delimited and had an increase in CD19+ cells within hyperplasic germinal nodes. The mean HBsAb levels in immunized control mice were greater than that in the exposed hosts (i.e., OD = 0.39 ± 0.03 vs. 0.11 ± 0.05, p < 0.01). HBsAb avidity dropped to a

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“…Megakaryocytic proliferation may result in increased production of platelets and increased risk for thromboembolic events [48] . Megakaryocytes arise from pluripotent hematopoietic stem cells capable of differentiation into all of the cell lines that reside in the bone marrow and produce blood cells.…”

Section: Discussionmentioning

confidence: 99%

Histological and immunohistochemical study on the potential toxicity of sliver nanoparticles on the structure of the spleen in adult male albino rats

Mazen

Saleh

Mahmoud

et al. 2017

Egyptian Journal of Histology

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Introduction: Silver nanoparticles (AgNPs) remain one of the controversial research areas regarding their distribution, dissolution and their toxicity to biological systems. Aim of the work: To evaluate the potential toxicity of different doses of AgNPs on the spleen of adult Albino rats. Materials and Methods: Forty adult male albino rats were divided into two main groups; control group: included ten rats that received intraperitoneal saline solution, daily, and experimental group: included thirty rats that received intraperitoneal AgNPs dissolved in saline solution, and were equally subdivided into three subgroups. Subgroup IIa received AgNPs (100 mg/ kg/d), subgroup IIb received AgNPs (500 mg/kg/d) and subgroup IIc received AgNPs (1000 mg/kg/d). After 28 days, rats were euthanized and the spleen specimens were prepared for light and electron microscope studies. Results: Spleen of AgNPs-treated rats revealed dose-dependent structural changes in the form of reduced sized white pulp follicles with depletion of lymphocytes, degeneration and apoptosis. Nanoparticles were seen in both white and red pulps. There was a highly statistical significant increase in the optical density of caspase-3 in subgroups IIa, IIb and IIc when compared to that of the control group. Conclusions: AgNPs induced structural alterations in the spleen with evidence of inflammatory response and oxidative stress. These effects were dose-dependent.

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Thrombocytosis induced in mice after subacute and subchronic V2O5 inhalation

Cited by 33 publications

References 10 publications

CD11c decrease in mouse thymic dendritic cells after vanadium inhalation

CD11c decrease in mouse thymic dendritic cells after vanadium inhalation

Vanadium Pentoxide Inhalation Provokes Germinal Center Hyperplasia and Suppressed Humoral Immune Responses

Histological and immunohistochemical study on the potential toxicity of sliver nanoparticles on the structure of the spleen in adult male albino rats

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