Thrombopoietin Activity in Mice Following Immune-Induced Thrombocytopenia

Abstract

Thrombopoietin levels in thrombocytopenic mice assayed by ⁷⁵Se-selenomethionine incorporation into blood platelets reached a maximum 12 h after the induction of an acute, immune thrombocytopenia; that was more than twice the value in control mice. The implications of this finding are discussed with reference to the kinetics of megakaryocyte production.

Icaritin Provokes Serum Thrombopoietin and Downregulates Thrombopoietin/MPL of the Bone Marrow in a Mouse Model of Immune Thrombocytopenia

“…The modeling method in the present study destroyed the platelet counts in the peripheral blood, reduced the platelet count, increased the hematopoietic function of the bone marrow, and compensated for the hyperplasia of megakaryocytes in the model group. These results are consistent with the literature [ 28 – 30 ]. The number of platelet counts in peripheral blood increased in all icaritin-treated groups, bone marrow TPO expression decreased, and bone marrow megakaryocyte production decreased.…”

Hematopoietic Deficiencies in c‐mpl and TPO Knockout Mice

“…TPO and c-mpl deficient mice have proven to be a useful model for studying the regulation of TPO production. The serum of c-mpl -/mice, like that of other thrombocytopenic animals, contains high levels of TPO [13,[33][34][35]. To explain the inverse relationship between platelet count and circulating TPO level, it was tempting to speculate that a sensing mechanism was responsible for detecting lower platelet counts and stimulating TPO transcription in a similar way that EPO transcription is regulated in response to anemia and low oxygen pressure [36][37][38].…”

Incorporation of H³-Leucine in the Mouse Kidney in Thrombocytopenia

“…The serum thrombopoietin level is elevated in APS-induced thrombo cytopenia as observed in experiments carried out by Schreiner and Lev in [12], N akeff and R oozendaal [9], and M arosi et al [6], The site of thrombopoietin production, however, is not known. Some data suggest that the kidney may play an important role in the production of thrombo poietin [4,7,8],…”