Thymol inhibits Candida albicans biofilm formation and mature biofilm

Braga, Pier Carlo; Culici, M.; Alfieri, M.; Sasso, M. Dal

doi:10.1016/j.ijantimicag.2007.12.013

Cited by 129 publications

(89 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Biofilms were washed and placed in a fixative consisting of 2% (vol/vol) glutaraldehyde in 0.15 M sodium cacodylate buffer (pH 7.2) for 2 h. The samples were rinsed twice in cacodylate buffer, garnished with 1% osmic acid for 2 h, dehydrated in an ascending ethanol series, treated with hexamethyl-disilazane, and dried overnight. The specimens were coated with gold and observed through a Philips XL-30 SEM (Philips) in high-vacuum mode (20,45).…”

Section: Methodsmentioning

confidence: 99%

Activity of Sanguinarine against Candida albicans Biofilms

Zhong

et al. 2017

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Candida albicans biofilms show resistance to many clinical antifungal agents and play a considerable contributing role in the process of C. albicans infections. New antifungal agents against C. albicans biofilms are sorely needed. The aim of this study was to evaluate sanguinarine (SAN) for its activity against Candida albicans biofilms and explore the underlying mechanism. The MIC 50 of SAN was 3.2 g/ ml, while Ն0.8 g/ml of SAN could suppress C. albicans biofilms. Further study revealed that Ն0.8 g/ml of SAN could decrease cellular surface hydrophobicity (CSH) and inhibited hypha formation. Real-time reverse transcription-PCR (RT-PCR) results indicated that the exposure of C. albicans to SAN suppressed the expression of some adhesion-and hypha-specific/essential genes related to the cyclic AMP (cAMP) pathway, including ALS3, HWP1, ECE1, HGC1, and CYR1. Consistently, the endogenous cAMP level of C. albicans was downregulated after SAN treatment, and the addition of cAMP rescued the SAN-induced filamentation defect. In addition, SAN showed relatively low toxicity to human umbilical vein endothelial cells, the 50% inhibitory concentration (IC 50 ) being 7.8 g/ml. Collectively, the results show that SAN exhibits strong activity against C. albicans biofilms, and the activity was associated with its inhibitory effect on adhesion and hypha formation due to cAMP pathway suppression.

show abstract

Section: Methodsmentioning

confidence: 99%

Activity of Sanguinarine against Candida albicans Biofilms

Zhong

et al. 2017

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…Its also bind adhesins that have been termed as the most important determinant of pathogenicity (Silva & Fernandes, 2010;Croxen & Finlay 2010;Vattem et al, 2007). The protective effect of terpens and terpenoids can be because of the presence of p-cymene -a precursor of carvacrol 1,8-cineole, tujen, a-pinene, o-kimen, p -kimen, γ-terpinene and β-phellandrene (Ultee et al, 2002;Braga et al, 2008).…”

Section: Histochemical Assaymentioning

confidence: 99%

Effect of Laurus nobilis extract on the functioning of liver against ccl4 induced toxicity

Gasparyan¹,

Tiratsuyan²,

Kazaryan³

et al. 2015

JEBAS

View full text Add to dashboard Cite

KEYWORDS Laurus nobilis Biochemical markers CCl4Hystochemistry Toxic hepatitis ABSTRACTIn present study effect of laurel leaf extract (Laurus nobilis) on biochemical parameters and histomorphology of rat liver induced by toxic damage of CCl4 was studied. Introduction of CCl4 in animals was always carried out in fatal to 36 hours. A single injection of the L. nobilis extract ssimultaneously with CCl4 leads to 100% survivability. To evaluate the degree of hepatocyte damage in experimental modeling hepatitis de Ritis index was used. It has been reported that intraperitoneal injection of a lethal dose of CCl4 activates hepatocyte damage in the key markers, such as alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, γ -glutamiltransferase, bilirubin, urea, albumin, glucose, cholesterol, triacylglycerides. Simultaneous treatment of CCl4 with Laurus nobilis leaves extract prevented death and exhibit a normalizing effect on the main indicators of liver damage and index de-Ritis. Result of the present study revealed that L. nobilis extract have capacity to manage metabolic and histological abnormalities of hepatocytes toxic damage induced by CCl4.

show abstract

“…Supporting the antifungal effect of thymol on mature biofilms of C. albicans, Braga et al (16) observed that after 6 h of incubation with thymol, biofilms of C. albicans showed reduction of 45.1% in their metabolic activity; and the same occurred after 12 h, but with 68% inhibition, and after 24 h of incubation, the percentage inhibition was 88.3%. These results are consistent with the aforementioned authors; however, in the current research the antifungal activity of thymol was demonstrated in much shorter exposures (5, 15 and 30 min) ( Table 1).…”

Section: Discussionmentioning

confidence: 93%

Cell Viability of Candida albicans Against the Antifungal Activity of Thymol

et al. 2014

View full text Add to dashboard Cite

Candida albicans is a commensal fungus, but circumstantially it may cause superficial infections of the mucous membranes, such as denture stomatitis, when a biofilm is formed on the surface of dental prostheses. This study evaluated the cell viability of C. albicans biofilms against the antifungal activity of thymol when compared with miconazole, by the fluorescence imaging using SYTO 9 and propidium iodide dyes, and counting of colony forming units. C. albicans standard strains (ATCC 11006) were used. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of drugs were determined by broth microdilution tests and the inoculum was standardized to match 0.5 on the McFarland scale (10 6 cfu/mL). Biofilms were grown on the surface of acrylic resin disks in parallel flow chambers from Sabouraud broth supplemented with 10% dextrose. For counting of colony forming units, the fungal solution was sequentially diluted and plated in Sabouraud dextrose agar. Data were analyzed using two-way ANOVA and Tukey's test (a=5%). Biofilms treated with thymol and miconazole presented low numbers of viable cells at the evaluated exposure times. There was statistically significant difference (p<0.05) when compared with control, and the mean value of the exposure times between miconazole and thymol did not differ significantly (p>0.05). In conclusion, both drugs have similar efficiency as antifungal agents against biofilms of C. albicans formed on acrylic surfaces.

show abstract

Thymol inhibits Candida albicans biofilm formation and mature biofilm

Cited by 129 publications

References 14 publications

Activity of Sanguinarine against Candida albicans Biofilms

Activity of Sanguinarine against Candida albicans Biofilms

Effect of Laurus nobilis extract on the functioning of liver against ccl4 induced toxicity

Cell Viability of Candida albicans Against the Antifungal Activity of Thymol

Contact Info

Product

Resources

About