Thymol Production from Callus Culture of Nigella sativa L.

Al-Ani, Nabeel K.

doi:10.3329/ptcb.v18i2.3649

Cited by 11 publications

(10 citation statements)

References 5 publications

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“…High callus formation was achieved from high-auxin-containing media, and auxincytokinin content balanced mediums. When similar previous studies were examined, a high rate of callus formation was obtained in cases where the mediums which had auxin-cytokinin were balanced and had high auxin content, similar to the results obtained from this study (AL-ANI, 2008;CHAUDHRY and AHMAD, 2013;QAMAR and SHIREEN, 2016). Callus formation was observed in almost all of the explants subject to this research in the medium containing 2.4-D, which is auxin hormone.…”

Section: Resultssupporting

confidence: 87%

In vitro propagation of black cumin (Nıgella satıva L.) plants

Uysal

2021

Genetika

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This study was carried out to determine in vitro development using Black cumin leaf and stem explants. ?ameli black cumin variety was used as plant material. Five different nutrient mediums (1. LS2.5, 2. MS, 3. MS + 0.5 mg.l-1 IAA, 4. MS + 0.5 mg.l-1 BAP, 5. MS + 0.5 mg.l-1 IAA + 0.5 mg.l-1 BAP) containing 30 g sugar were used in this study. As a result of the research, 100% callus formation was detected in the stem explants cultured in the number 1 and number 5 mediums. These were followed by stem explants cultured in medium 4 with a success rate of 96%. Of this rate, 66% was shoot formation, and 30% was callus formation. Direct shoot regeneration was performed only on stem explants cultured in mediums 4 and 3, with a 66% success rate in medium four and a 36% success rate in medium 3. The highest plant regenerations from calluses were gained from stem explants (273.3%) in medium 4, followed by calluses gained from leaf explants (262.5%) in the same medium. These were followed by cultures in medium 3, with calluses derived from stem explants (255%) and leaf explants (150%). No plant regeneration was determined from calluses gained in the medium 1. Thus it is evident that high auxin content and auxin-cytokinin balanced mediums encouraged callus formation in the black cumin plants. The addition of only IAA or BAP to the medium promoted shoot formation in the stem explants, but direct shoot regeneration was not thereby achieved from the leaf explants. These results show that, for in vitro clonal propagation studies done on black cumin plants, a high auxin containing medium is preferable if the aim is callus formation. If the aim is direct shoot regeneration, BAP or other cytokinin-containing medium is preferred.

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Section: Resultssupporting

confidence: 87%

In vitro propagation of black cumin (Nıgella satıva L.) plants

Uysal

2021

Genetika

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“…Based on Table 5 showed that flavonoid content from callus culture lower than leaves of black cumin. Callus produced in vitro can be understood due to the production of metabolites secondary in vitro callus is influenced by various factors including the composition of the medium used and the applied growth regulator [2,[8][9].…”

Section: Flavonoid Contentmentioning

confidence: 99%

“…As an herbal plant, the seeds of black cumin are able to overcome various types of diseases including viruses. According to [2,3] black cumin has many benefits in overcoming various diseases such as anticancer, immunity-enhancing, antihistamine, anti-inflammatory, microbial and anti-tumour activity. Black cumin also contains secondary metabolite compounds.…”

Section: Introductionmentioning

confidence: 99%

Production of biomass and flavonoids in black cumin tissue culture

Jannah,

Damanik

2023

IOP Conf. Ser.: Earth Environ. Sci.

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As an herbal plant, the many parts of black cumin are able to cope with various kinds of diseases including viruses. The need to provide bioactive ingredients in that time short is not accompanied by the availability of land and germplasm. With the existence of these problems, tissue culture techniques can be used to addressing the tissue. Callus culture is a heterotrophic cultivation. By paying attention to the large content of secondary metabolite compounds useful ones can be produced through callus induction techniques. Currently, the study has started using MS media, and modification of the type of tissue culture media by adding Picloram of 1 ppm combination with GA3 of 2 ppm can increased the percentage of callus growth. likewise affects the formation of phenolic and flavonoid content of black cumin callus.

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“…Chaudhry et al and Hoseinpanahi et al concluded that combination of Kn + NAA and BAP + IAA enhanced terpenoid and thymol production in suspension culture of N. sativa [72,73]. Al-Ani also reported that 2,4-D and Kn initiated callus formation from leaf explant of Nigella sativa with enhanced thymol concentration [74]. By enhancing metabolic content the antioxidant activity of culture is also increased, Further, TDZ + NAA enhanced biomass and antioxidant activity of callus of Nigella sativa [77].…”

Section: Effects Of Pgrsmentioning

confidence: 99%

“…Some recent examples of medicinal secondary metabolites in suspension cultures include zerumbone production in Zingiber zerumbet, [102] ursolic acid production in Eriobotrya japonica, [103] lutein and tocopherol in carrot, [104] rosmarinic acid in Satureja khuzistanica [105] and taxane in Taxus chinensis [106], enhanced terpenoid and thymol production in N. sativa [72]. Al-Ani reported that from callus culture of N. sativa higher thymol concentration was extracted [74]. Enhanced biomass and antioxidant activity showed by the Cotyledon derived callus culture of Nigella sativa [77].…”

Section: Plant Cell Cultures Strategies For Phytochemical Productionmentioning

confidence: 99%

Biotechnological approaches for production of bioactive secondary metabolites in Nigella sativa: an up-to-date review

Kazmi

Khan

Ali

2019

International Journal of Secondary Metabolite

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Medicinal and aromatic plants and their refined natural products have gained global attraction for their therapeutic potential against many human diseases. Nigella sativa is a medicinally important plant, commonly known as Black cumin or Black seed is a dicotyledon plant of the Ranunculaceae family. It is in common use for a longer time in history as preservative and spice and has also been extensively utilized by different communities around the globe. Black cumin has been an eminent component of traditional medicine systems like Unani and Tibb, Ayurveda and Siddha. Its biological activities include antidiarrheal, analgesic, antibacterial, liver tonic, diurectic, digestive agent and to treat several skin disorders. Furthermore, the therapeutic properties also include antidiabetic, anticancer, antihypertensive, anti-inflammatory, hepatoprotective, spasmolytic and bronchodialator. This is all because of its miraculous healing power that it has been ranked as top ranked, among evidence based herbal medicines. The literature supports that the pharmacological activities of Nigella sativa are mainly because of the essential oil and its constituents particularly thymoquinone. The current review is an attempt to present a detailed literature survey regarding chemical composition, phytochemistry, therapeutic potential and biotechnological approaches to enhance the medicinal potential of this valuable plant.

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Thymol Production from Callus Culture of Nigella sativa L.

Cited by 11 publications

References 5 publications

In vitro propagation of black cumin (Nıgella satıva L.) plants

In vitro propagation of black cumin (Nıgella satıva L.) plants

Production of biomass and flavonoids in black cumin tissue culture

Biotechnological approaches for production of bioactive secondary metabolites in Nigella sativa: an up-to-date review

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