Thyrocalcitonin. I. Isolierung und Charakterisierung wirksamer Peptide aus Schweineschilddrüsen

Kahnt, F. W.; Riniker, B.; MacIntyre, I.; Neher, R.

doi:10.1002/hlca.19680510125

Cited by 32 publications

(5 citation statements)

References 14 publications

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“…In porcine CT methionine occupies position 25. It has previously been re¬ ported that the methionine sulphoxide derivative of porcine -CT (porcine /?-CT) has the same hypocalcaemic activity as porcine -CT (Kahnt, Riniker, Maclntyre & Neher, 1968). This has been confirmed by our experiments.…”

Section: Specificitymentioning

confidence: 99%

Acute Effects of Synthetic Porcine Calcitonins on the Renal Excretion of Magnesium, Inorganic Phosphate, Sodium and Potassium

Sp¹,

Buchanan-Lee²,

Ew³

et al. 1971

Journal of Endocrinology

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Synthetic porcine calcitonin (\g=a\-calcitonin) and its methionine-sulphoxide derivative (\g=b\-calcitonin) were given by intravenous infusion to conscious male rats. \g=a\-Calcitonininactivated by performic acid oxidation was used as a control.Microgram doses of \g=a\-calcitonin produced a dose-dependent decrease in the renal excretion of magnesium. The effect was not due to a secondary release of parathyroid hormone since it was also seen in parathyroidectomized animals.A marked increase in the renal excretion of inorganic phosphate, sodium and potassium preceded the change in magnesium excretion in parathyroidectomized rats. It is concluded that the phosphaturia and natriuresis previously described after administration of extracted calcitonin preparations are true effects of the hormone.The effect of \g=b\-calcitonin was indistinguishable from that of \g=a\-calcitonin.

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Section: Specificitymentioning

confidence: 99%

Acute Effects of Synthetic Porcine Calcitonins on the Renal Excretion of Magnesium, Inorganic Phosphate, Sodium and Potassium

Sp¹,

Buchanan-Lee²,

Ew³

et al. 1971

Journal of Endocrinology

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“…with butanol-acetic acid-water solvent using the method of Kahnt, Riniker, Maclntyre & Neher (1968). Concentrations in the gland are expressed in terms of MRC units /g.…”

Section: Embryology and Morphologymentioning

confidence: 99%

Endocrine Control of Calcium Homeostasis

Copp¹

1969

Journal of Endocrinology

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Two endocrine glands of branchial origin are involved in regulation of calcium metabolism\p=m-\the parathyroids which secrete parathormone (parathyroid hormone) in response to hypocalcaemia, and the ultimobranchial glands which release calcitonin (thyrocalcitonin) during hypercalcaemia. The ultimobranchial glands are distinct organs in most vertebrates but in mammals the cells become embedded in the internal parathyroid and thyroid where they constitute the parafollicular 'C' cells. Calcitonin can be extracted from mammalian thyroid (hence the alternative name, thyrocalcitonin) but it is present in much higher concentrations in the ultimobranchial glands of lower vertebrates. In mammals, both hormones appear to act primarily on bone resorption and calcium mobilization from the skeleton, which is stimulated by parathormone and inhibited by calcitonin. Secretion or release of both hormones is determined by the level of calcium in the blood, and the two constitute a dual endocrine control comparable to that of insulin and glucagon in regulation of blood glucose. The ultimobranchials are the older glands phylogenetically for they are found in sharks and bony fishes, while the parathyroids appear first in amphibians.

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“…Extraction of the acetone powder of thyroid glands with dilute acid was usually followed by recovery of crude calcitonin through precipitation by salts, organic solvents, or trichloracetic acid. Preparation of substantially pure hormone has now been reported from several laboratories (9)(10)(11)(12)(13)(14). Purification of crude preparations has been accomplished chiefly by repeated gel filtrations with agents such as the Sephadexes; countercurrent distribution and preparative disc electrophoresis (9,11,14) have also been useful.…”

Section: Isolation and Purificationmentioning

confidence: 98%

“…Purification of crude preparations has been accomplished chiefly by repeated gel filtrations with agents such as the Sephadexes; countercurrent distribution and preparative disc electrophoresis (9,11,14) have also been useful. The purified hormone represents about a 44,000-fold pnrification from the dried, defatted glands, and has a specific activity of 150 to 200 Medical Research Council (MRC) units per milligram (9,12,14).…”

Section: Isolation and Purificationmentioning

confidence: 99%