Thyroid peroxidase antibodies in children with autoimmune thyroiditis.

Väkevä, Antti; Kontiainen, Sirkka; Miettinen, Aaro; Schlenzka, A; Mäenpää, J

doi:10.1136/jcp.45.2.106

Cited by 11 publications

(4 citation statements)

References 22 publications

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“…The data confirmed the reports of Chuang and Tsai and their coworkers, and were in accordance with previous reports, indicating no ethnic difference in the association of ATD and Chinese type 1 diabetes mellitus. High titers of anti-TPO were highly suggestive of ATD, and correlated well with thyroid dysfunction (30). The positive anti-TPO titers in our type 1 diabetic patients showed wide distribution.…”

Section: Discussionmentioning

confidence: 80%

Autoantibodies to thyroid peroxidase in patients with type 1 diabetes in Taiwan

Chang

Huang²,

Chuang³

1998

European Journal of Endocrinology

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Objective: Type 1 diabetes mellitus is frequently associated with autoimmune thyroid disease (ATD). Genetic susceptibility to autoantibody formation in association with ATD and type 1 diabetes mellitus has been described with varying frequencies, but there is still debate about the situation in the Chinese population. We have, therefore, investigated the prevalence of anti-thyroid peroxidase (anti-TPO) in type 1 diabetic patients, and compared the effect of anti-glutamate decarboxylase (anti-GAD) on the thyroid autoimmunity in patients with type 1 diabetes mellitus in Taiwan. Subjects and methods: Two hundred and forty-three subjects with type 1 diabetes mellitus and seventy unrelated normal controls were recruited for the detection of anti-TPO. Two hundred and seventeen sera from two hundred and forty-three type 1 diabetic patients were tested for anti-GAD. RIA and immunoprecipitation were used for anti-TPO and anti-GAD detection respectively. Results: The intra-assay and interassay coefficients of variation of anti-TPO detected by the RIA method ranged from 5.5% to 11.1%. Among 243 type 1 diabetic patients, 53 (21.8%) were positive for anti-TPO. Compared with those without thyroid autoimmunity, there was a female preponderance for the type 1 diabetic patients with thyroid autoimmunity (female:male, 99:91 vs 37:16 respectively). Among the type 1 diabetic patients with thyroid autoimmunity, anti-TPO tended to occur in those of older age or with long-standing disease. The frequency of anti-GAD was 45.6% (99 of 217), without gender preponderance (males:females, 18.0% vs 27.6%). Compared with those with negative anti-GAD, no significant difference of anti-TPO positivity for the type 1 diabetic patients with positive anti-GAD was found. Conclusion: Our data indicated that the RIA method for anti-TPO detection is sensitive and precise for routine clinical use. The presence of anti-TPO in 21.8% of our type 1 diabetic patients confirmed the strong association of ATD and type 1 diabetes mellitus without ethnic differences. The absence of correlation between anti-TPO and anti-GAD in our type 1 diabetic patients suggested genetic heterogeneity in the role of autoimmunity of type 1 diabetes mellitus and ATD among races.

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Section: Discussionmentioning

confidence: 80%

Autoantibodies to thyroid peroxidase in patients with type 1 diabetes in Taiwan

Chang

Huang²,

Chuang³

1998

European Journal of Endocrinology

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“…Nonetheless, if the antibodies are negative, the diagnosis of HT can still be confirmed by FNAC. 22,35 Our study highlights the importance of FNAC as a simple and reliable modality in the diagnosis of HT, which often may be missed on mere serologic examination. Though serology is a useful adjunct in the diagnosis of HT, in antibody-negative cases, or in biochemically euthyroid patients, FNAC remains the "gold standard" for diagnosing HT.…”

Section: Significant Serologic Findingsmentioning

confidence: 99%

Cytomorphologic Study of Hashimoto’s Thyroiditis and Its Serologic Correlation

et al. 2009

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“…In total, 1333 sera (from 558 patients) were assayed by RIA-1 and 8770 sera (from 3674 patients) were assayed by RIA-2. After incubation, the procedure for the two assays is the same: the radio¬ active mixture is aspirated and the tubes carefully results is unclear, but already reported by others (10). Briefly, the serum prediluted 1 : 51 (RIA-1) or 1:21 (RIA-2) is incubated with 125I-labeled TPO (50 µ\ corresponding to 30 000-50000cpm) for [18][19][20][21][22] h (RIA-1) or 2 h (RIA-2) at room temperature in tubes coated with a monoclonal antibody directed against a major autoepitope of TPO.…”

Section: Anti-tpo Assaysmentioning

confidence: 99%

“…Few other studies (8)(9)(10), however, are available on the clinical validation of this assay, with particular regard to the comparison with other differently designed RIAs. This prompted us to re-evaluate the relationship between anti-TPO detected by commercial RIA and anti-M by passive hemagglutination in a very large series of unselected sera with the purpose of studying the incidence and identifying the causes of discrepancies.…”

mentioning

confidence: 99%

False negative results observed in anti-thyroid peroxidase autoantibody determination by competitive radioimmunoassays using monoclonal antibodies

Mariotti

Barbesino

Caturegli

et al. 1994

European Journal of Endocrinology

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Anti-TPO by competitive monoclonal antibody-assisted RIA is negative in a minority of sera of patients with autoimmune thyroid disease and positive anti-M. This could be accounted for by anti-Tg producing false positives in the anti-M assay and by a subset of anti-TPO not competing with the monoclonal antibodies in the RIA. When autoimmune thyroid disease is suspected on clinical grounds, a negative anti-TPO test with a competitive RIA should be confirmed always by a non-competitive assay.

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Thyroid peroxidase antibodies in children with autoimmune thyroiditis.

Abstract: Aims: To compare the prevalence of thyroid peroxidase antibodies in 25 children with autoimmune thyroid disorders and in 41 children and young adults with type 1 diabetes, and to test the prevalence of thyrotropin receptor antibodies.

Cited by 11 publications

References 22 publications

Autoantibodies to thyroid peroxidase in patients with type 1 diabetes in Taiwan

Autoantibodies to thyroid peroxidase in patients with type 1 diabetes in Taiwan

Cytomorphologic Study of Hashimoto’s Thyroiditis and Its Serologic Correlation

False negative results observed in anti-thyroid peroxidase autoantibody determination by competitive radioimmunoassays using monoclonal antibodies

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