THz irradiation inhibits cell division by affecting actin dynamics

Yamazaki, Shota; Ueno, Yuya; Hosoki, Ryosuke; Saito, Takanori; Idehara, T.; Yamaguchi, Yuusuke; Otani, Chiko; Ogawa, Yuichi; Harata, Masahiko; Hoshina, Hiromichi

doi:10.1371/journal.pone.0248381

Cited by 15 publications

(7 citation statements)

References 54 publications

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“…A conclusion was made, based on the changes in the intensity of the signal of a membrane-selective fluorescent dye FM4-64 upon the irradiation. Moreover, it was shown that the THz rays influenced F-actin polymerization both in vitro [ 24 ] and in vivo, in living cells [ 13 , 25 ]. Unfortunately, the effect described in viable cells was quite different—the destruction of actin filaments [ 25 ] and the induction of F-actin and its polarization [ 13 ] was observed.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, it was shown that the THz rays influenced F-actin polymerization both in vitro [ 24 ] and in vivo, in living cells [ 13 , 25 ]. Unfortunately, the effect described in viable cells was quite different—the destruction of actin filaments [ 25 ] and the induction of F-actin and its polarization [ 13 ] was observed. Most likely, similar observations could be interpreted differently.…”

Section: Discussionmentioning

confidence: 99%

“…Others observed induced DNA damage in irradiated artificial skin (0.1–2 THz, I = 57 mW/cm 2 , t = 2 min) [ 9 ], phosphorylation of H2A histone family member X in irradiated human primary skin fibroblasts (1.5–3 THz, I—0.84 mW/cm 2 —32 GW/cm 2 , t—10–180 min) [ 10 ], genomic instability in human lymphocytes (0.1 THz, I = 0.031 mW/cm 2 , t = 1, 2, and 24 h) [ 11 ], aneuploidy in primary adult skin fibroblasts (0.10–0.15 THz, I = 0.40 mW/cm 2 ) [ 12 ], and disturbances in cell division (inhibition of cytokinesis) in human cervical cancer HeLa cells (0.28–0.46 THz, I = 6–125 mW/cm 2 , t = 1 h) [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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A Comparative Study on the Viability of Normal and Cancerous Cells upon Irradiation with a Steady Beam of THz Rays

Kovalevska

Голенков

Kulahina

et al. 2022

Life

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Terahertz (THz) electromagnetic radiation is commonly used in astronomy, security screening, imaging, and biomedicine, among other applications. Such approach has raised the question of the influence of THz irradiation on biological objects, especially the human body. However, the results obtained to date are quite controversial. Therefore, we performed a comparative study on the viability of normal cells and cancer cells upon irradiation with a steady beam of THz rays. We used human peripheral blood mononuclear cells and cancer cell lines. Primary human mononuclear blood cells (monocytes, and B-, and T-cells) showed an increased death rate, determined by cell counting and fluorescence microscopy, upon 0.14 THz irradiation. The effect of THz radiation was different among malignant cells of B- and T-cell origin (Ramos and Jurkat cells) and epithelial cancer cells (MCF7 and LNCaP). This was demonstrated by cell counting and by the alamarBlue assay. In conclusion, THz radiation can result in the death of human primary and malignant cells. However, the mechanism of this phenomenon is largely unknown. Hence, more work should be done to shed some light on the mechanism of action of THz irradiation in living organisms to enhance technologic developments.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Comparative Study on the Viability of Normal and Cancerous Cells upon Irradiation with a Steady Beam of THz Rays

Kovalevska

Голенков

Kulahina

et al. 2022

Life

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“…For long-wavelength radiation, where the photon energy is far below that needed to induce chemical reactions (e.g., bond energies), and even below that of thermal photons at ambient temperature, one might wonder if any nonthermal mechanisms exist that can induce any biologically relevant changes. This question has been the topic of extensive studies for many years at lower frequencies and also, more recently, in the THz regime. − In most of the cases when the data have been interpreted to show a nonthermal effect arising from exposure to THz light, the experiments did not employ the needed controls and statistics to rule out a more prosaic interpretation, such as heating. − These are exceedingly challenging experiments, as (for example) it is almost impossible to rule out the possibility of spatially nonuniform transient heating with significant temperature variations on a subcellular length scale. Most notably, none of the purported nonthermal effects have ever been verified by a second independent research laboratory, and no plausible underlying mechanism has been proposed for how such effects could be induced by photons with only a few millivolts of energy.…”

Section: Introductionmentioning

confidence: 99%

Perspective on Terahertz Applications in Bioscience and Biotechnology

2022

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Within the field of terahertz science and technology, one of the most active areas of current research focuses on the intersection of terahertz measurements and methods with the world of biology and medicine. Current activities revolve around numerous diverse questions, ranging from studies of the vibrational spectra of biomolecules and macromolecular complexes to biosensing to medical diagnostics based on noninvasive imaging techniques. Unlike many other areas in which terahertz science is now making inroads, this research domain has been plagued with a number of misleading ideas, which originated at least two decades ago and continue to crop up in current literature. In the worst case, these unfortunate notions can distract from, and even obscure, fascinating and meaningful results. The purpose of this Perspective is to highlight a few of these mistaken concepts and, more importantly, to distinguish them from the many interesting works that continue to emerge from the fruitful marriage of terahertz with biology and medicine.

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“…Low-frequency THz radiation (0.46 THz) enhances the polymerization of purified actin in vitro, and by similarly affecting actin polymerization in vivo, inhibits human cell division. In an aqueous medium (~1 mm thick) at an irradiation frequency of 4 THz, both in vitro and in live cells, actin filaments disintegrated, possibly as the result of a shock wave [ 15 , 16 , 17 ]. The exposure of neuron-like PC 12 cells to THz radiation (0.3–19.5 THz) for 10 min temporarily increased the permeability of the cell membranes [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Specific Features of the Proteomic Response of Thermophilic Bacterium Geobacillus icigianus to Terahertz Irradiation

Bannikova

Khlebodarova

Vasilieva

et al. 2022

IJMS

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Studying the effects of terahertz (THz) radiation on the proteome of temperature-sensitive organisms is limited by a number of significant technical difficulties, one of which is maintaining an optimal temperature range to avoid thermal shock as much as possible. In the case of extremophilic species with an increased temperature tolerance, it is easier to isolate the effects of THz radiation directly. We studied the proteomic response to terahertz radiation of the thermophilic Geobacillus icigianus, persisting under wide temperature fluctuations with a 60 °C optimum. The experiments were performed with a terahertz free-electron laser (FEL) from the Siberian Center for Synchrotron and Terahertz Radiation, designed and employed by the Institute of Nuclear Physics of the SB of the RAS. A G. icigianus culture in LB medium was THz-irradiated for 15 min with 0.23 W/cm2 and 130 μm, using a specially designed cuvette. The life cycle of this bacterium proceeds under conditions of wide temperature and osmotic fluctuations, which makes its enzyme systems stress-resistant. The expression of several proteins was shown to change immediately after fifteen minutes of irradiation and after ten minutes of incubation at the end of exposure. The metabolic systems of electron transport, regulation of transcription and translation, cell growth and chemotaxis, synthesis of peptidoglycan, riboflavin, NADH, FAD and pyridoxal phosphate cofactors, Krebs cycle, ATP synthesis, chaperone and protease activity, and DNA repair, including methylated DNA, take part in the fast response to THz radiation. When the response developed after incubation, the systems of the cell’s anti-stress defense, chemotaxis, and, partially, cell growth were restored, but the respiration and energy metabolism, biosynthesis of riboflavin, cofactors, peptidoglycan, and translation system components remained affected and the amino acid metabolism system was involved.

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THz irradiation inhibits cell division by affecting actin dynamics

Cited by 15 publications

References 54 publications

A Comparative Study on the Viability of Normal and Cancerous Cells upon Irradiation with a Steady Beam of THz Rays

A Comparative Study on the Viability of Normal and Cancerous Cells upon Irradiation with a Steady Beam of THz Rays

Perspective on Terahertz Applications in Bioscience and Biotechnology

Specific Features of the Proteomic Response of Thermophilic Bacterium Geobacillus icigianus to Terahertz Irradiation

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