Tianma Gouteng Decoction Exerts Pregnancy-Protective Effects Against Preeclampsia via Regulation of Oxidative Stress and NO Signaling

Jin, Meiyuan; Cao, Bin; Lin, Chao; Li, Jiayong; Chen, Nansheng; Ren, Qianlei; Xu, Shouying; Tang, Chao

doi:10.3389/fphar.2022.849074

Cited by 11 publications

(9 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CCK‐8 assays were performed to determine cell viabilities as described previously 16 . Briefly, cells at 80% confluence were re‐seeded into 96‐well plates and were transfected with ALKBH1‐expressing plasmid, ALKBH1‐shRNA, GPR137‐shRNA or control vectors.…”

Section: Methodsmentioning

confidence: 99%

“…Isolation of total RNA from RCC cell lines, Caki-1, 786-O, ACHN, Osrc2, A498 and 769-P, was conducted with a TRIzol reagent (Takara Biotechnology, Beijing, China) according to the manufacturer's protocol as described previously. 16 For generating first-strand cDNA, 5 μg total RNA in a volume of 20 μL was reversely transcribed by using SuperScript III reagent (Life Technologies) and the oligo-(deoxythymidine) primer with incubation at 42°C for 1 h. Subsequently, the mRNA expression of target genes was measured by qRT-PCR experiments with the synthesized cDNA. The relative amounts of the mRNA expression of the target genes were normalized to the corresponding GAPDH levels, respectively, and consequently, the relative difference in mRNA expression was calculated by 2 −ΔΔCt method as described previously.…”

Section: Total Rna Isolation Reverse Transcription and Quantitative R...mentioning

confidence: 99%

“…| Cell-counting kit-8 (CCK-8) assays CCK-8 assays were performed to determine cell viabilities as described previously. 16 Briefly, cells at 80% confluence were re-seeded into 96-well plates and were transfected with ALKBH1-expressing plasmid, ALKBH1-shRNA, GPR137-shRNA or control vectors. After culture for 48 h or 72 h, cells in each well were subjected to 20 μL CCK-8 solution (Beyotime) for 2 h. Cell viability was then measured by examining the absorbance at 450 nm via a multimode plate reader (Bio-Tek Instruments).…”

Section: 7mentioning

confidence: 99%

See 2 more Smart Citations

ALKBH1 contributes to renal cell carcinoma progression by reducing N6‐methyladenine of GPR137

Lin

Zhu

Chen

et al. 2023

Eur J Clin Investigation

Self Cite

View full text Add to dashboard Cite

Background Renal cell carcinoma (RCC) accounts for approximately 4% of all adult malignancies with high mortality worldwide. Although conventional chemotherapy and radiotherapy treatment has been applied for RCC in clinic, the mortality rate of patients is increasing each year, and patients with metastatic RCC are still suffering from poor prognosis. Thus, further investigation of the molecular mechanisms responsible for the development and progression of RCC is of particular importance. Methods Total of 10 pairs of RCC tissues and adjacent nontumor tissues were collected for examination of ALKBH1 and GPR137 expression. The correlations between ALKBH1 and GPR137 expression in RCC patient were assessed by GEPIA online tool and were analyzed using auto best cutoff. The human RCC cell lines Caki‐1, 786‐O, ACHN, Osrc2, A498, and 769‐P, were used for mechanistic investigation. Results Here, we report that the expression of AlkB homologue 1 (ALKBH1) is upregulated in RCC tissues, which is correlated with G‐protein‐coupled receptor 137 (GPR137) expression. The elevated expression of ALKBH1 is associated with RCC cell malignant characteristics, including cell proliferation and movement (migration and invasion). Mechanistic investigation further reveals that ALKBH1 reduces m6A levels of GPR137 mRNA in RCC cells, which upregulates GPR137 mRNA levels, resulting in the increased GPR137 protein expression subsequently and the enhanced RCC cell biological actions consequently. In contrast, the suppression of GPR137 effectively alleviates the ALKBH1‐induced malignancies of RCC cells. Conclusion Our results indicate that ALKBH1–GPR137 axis might be used as a potential therapeutic target in RCC, contributing to finding new prognostic biomarkers for RCC at an early stage.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Total Rna Isolation Reverse Transcription and Quantitative R...mentioning

confidence: 99%

Section: 7mentioning

confidence: 99%

See 1 more Smart Citation

ALKBH1 contributes to renal cell carcinoma progression by reducing N6‐methyladenine of GPR137

Lin

Zhu

Chen

et al. 2023

Eur J Clin Investigation

Self Cite

View full text Add to dashboard Cite

show abstract

“…Vaginal smears were daily obtained from female mice until the smears showed that they were oestrous. A PE mouse model was induced by subcutaneous injection of L-NAME [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

Ultrasound and microbubble-mediated delivery of miR-424-5p has a therapeutic effect in preeclampsia

Wang

Sun

et al. 2023

Biol Proced Online

View full text Add to dashboard Cite

Objective To determine the influence of ultrasound/microbubble-mediated miR-424-5p delivery on trophoblast cells and the underlying mechanism. Methods Blood pressure and 24-h proteinuria of patients with preeclampsia (PE) were measured as well as the levels of miR-424-5p and amine oxidase copper containing 1 (AOC1) in placental tissues. HTR-8/Svneo and TEV-1 cells were subjected to cell transfection or ultrasonic microbubble transfection for determination of the expression of miR-424-5p, AOC1, β-catenin and c-Myc as well as cell proliferation, apoptosis, migration and invasiveness. The concentrations of placental growth factor (PLGF), human chorionic gonadotropin (β-hCG) and tumor necrosis factor-α (TNF-α) were measured in HTR-8/Svneo and TEV-1 cells. RNA immunoprecipitation (RIP) and dual luciferase reporter assay detected the binding of miR-424-5p to AOC1. A PE mouse model was induced by subcutaneous injection of L-NAME, where the influence of ultrasound/microbubble-mediated miR-424-5p delivery was evaluated. Results miR-424-5p was downregulated while AOC1 was upregulated in the placental tissues from PE patients. Overexpression of miR-424-5p activated Wnt/β-catenin signaling pathway and promoted the proliferation of HTR-8/Svneo and TEV-1 cells as well as enhanced the migratory and invasive behaviors. AOC1 overexpression partly eliminated the effects of miR-424-5p on HTR-8/Svneo and TEV-1 cells. Ultrasound and microbubble mediated gene delivery enhanced the transfection efficiency of miR-424-5p and further promoted the effects of miR-424-5p in trophoblast cells. Ultrasound/microbubble-mediated miR-424-5p delivery alleviated experimental PE in mice. Conclusion Ultrasound and microbubble-mediated miR-424-5p delivery targets AOC1 and activates Wnt/β-catenin signaling pathway, thus promoting the aggressive phenotype of trophoblast cells, which indicating that miR-424-5p/AOC1 axis might be involved with PE pathogenesis.

show abstract

“…The PE rat model was established by subcutaneous injection of NG-nitro-L-arginine methyl ester (L-NAME). 32,33 Different working groups have previously established the PElike model that has been used in this study. [34][35][36] It is induced by the administration of L-NAME, which is an inhibitor of NO production, as it inhibits the NOS enzyme, prevents endothelium-dependent relaxation, and produces an increase in the blood pressure.…”

Section: Animal Modelmentioning

confidence: 99%

Suppression of circular RNA serum and glucocorticoid‐induced kinase 1 elevates antioxidant molecules and angiogenesis in trophoblast cells to attenuate preeclampsia via microRNA‐508‐3p to target and restrain PUM homolog 1

Lu,

Zheng,

Pan

et al. 2023

J of Obstet and Gynaecol

View full text Add to dashboard Cite

AimPreeclampsia (PE) is a pregnancy‐specific syndrome characterized by hypertension and proteinuria. Recently, multiple circular RNAs (circRNAs) were considered latent clinical diagnostic markers or therapeutic targets. This study was to explore the impact of circRNA serum and glucocorticoid‐induced kinase 1 (SGK1) on PE via influencing the microRNA (miR)‐508‐3p/PUM homolog 1 (PUM1) axis.MethodsPlacental tissues of 34 pregnant women with PE and 34 normal pregnant women were collected to measure circRNA SGK1 (circSGK1), miR‐508‐3p, and PUM1. Human placental trophoblasts HTR‐8/SVneo were transfected with plasmids, thereafter to observe proliferation, migration, invasion, and apoptosis, analyze antioxidant molecules Troxerutin (TXN), Glutamate‐cysteine ligase catalytic subunit (GCLC), NAD (P) H‐quinone oxidoreductase 1 (NQO1), and determine angiogenesis. After the construction of the PE rat model, antioxidant molecules TXN, GCLC, and NQO1, vascular‐associated factor vascular endothelial growth factor A (VEGF‐A), and histopathological conditions were tested. Examination of the binding of circSGK1 and miR‐508‐3p with PUM1 was performed.ResultsOur data showed that circSGK1 expression was elevated in the placenta of patients with PE. Silenced circSGK1 or elevated miR‐508‐3p promoted the growth and antioxidant molecules and angiogenesis in trophoblast cells; CircSGK1 combined with miR‐508‐3p, and miR‐508‐3p targeted PUM1.ConclusionsIn summary, suppression of circSGK1 augments antioxidant molecules and angiogenesis in trophoblast cells to attenuate PE via miR‐508‐3p to target PUM1.

show abstract

Tianma Gouteng Decoction Exerts Pregnancy-Protective Effects Against Preeclampsia via Regulation of Oxidative Stress and NO Signaling

Cited by 11 publications

References 58 publications

ALKBH1 contributes to renal cell carcinoma progression by reducing N6‐methyladenine of GPR137

ALKBH1 contributes to renal cell carcinoma progression by reducing N6‐methyladenine of GPR137

Ultrasound and microbubble-mediated delivery of miR-424-5p has a therapeutic effect in preeclampsia

Suppression of circular RNA serum and glucocorticoid‐induced kinase 1 elevates antioxidant molecules and angiogenesis in trophoblast cells to attenuate preeclampsia via microRNA‐508‐3p to target and restrain PUM homolog 1

Contact Info

Product

Resources

About