Tibial Osteotomy as a Mechanical Model of Primary Osteoarthritis in Rats

Britzman, David; Igah, Ibidumo; Eftaxiopoulou, Theofano; Macdonald, Warren; Bull, Anthony M.J.

doi:10.1038/s41598-018-23405-3

Cited by 8 publications

(7 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, when the knees of animal received artificial induction of OA by ACL and medial menisectomy, they experienced the unbalance of mechanical loading stress by meniscal destabilization. Eventually, the medial tibia articular regions could develop severe coronal and trabecular mal-alignments, which were considered as the key factor for OA initiation [46][47][48][49]. Therefore, we theorized that ESWT application on medial tibia subchondral bone in early OA stage could triggered the appropriate tissue repair factors, such as BMPs, Wnt and VEGF and that might eventually alleviated OA progression [50].…”

Section: Discussionmentioning

confidence: 99%

Pathological, Morphometric and Correlation Analysis of the Modified Mankin Score, Tidemark Roughness and Calcified Cartilage Thickness in Rat Knee Osteoarthritis after Extracorporeal Shockwave Therapy

Cheng¹,

Wang²,

Siu³

et al. 2022

Int. J. Med. Sci.

View full text Add to dashboard Cite

The paper displayed the pathological changes and relationships of the modified Mankin score, tidemark roughness and calcified cartilage (CC) thickness by extracorporeal shockwave therapy (ESWT) (0.25 mJ/ mm 2 with 800 impulses) on different positions of the medial and lateral rat knee OA joint. After the experiments, the articular cartilage was assessed using histomorphometry, image analysis and statistical method. In the micro-CT analysis, ESWT on medial groups were better than lateral groups in the trabecular volume and trabecular number. The data showed a strong negative correlation between the modified Mankin score and tidemark roughness (r = −0.941; P < 0.001). In terms of the relationship of tidemark roughness with CC thickness, the medial and Sham groups showed a significant negative correlation (r = −0.788, P = 0.022). Additionally, the Euclidean distance derived from 3D scatter plot analysis was an indicator of chondropathic conditions, exhibiting a strong correlation with OA stage in the articular cartilage of the femur (r = 0.911, P < 0.001) and tibia (r = 0.890, P < 0.001) after ESWT. Principle component analysis (PCA) further demonstrated that ESWT applied to medial locations had a better outcome than treatment at lateral locations for knee OA by comparing with Sham and OA groups, and CC thickness was the most important factor affecting hyaline cartilage repair after ESWT.

show abstract

Section: Discussionmentioning

confidence: 99%

Pathological, Morphometric and Correlation Analysis of the Modified Mankin Score, Tidemark Roughness and Calcified Cartilage Thickness in Rat Knee Osteoarthritis after Extracorporeal Shockwave Therapy

Cheng¹,

Wang²,

Siu³

et al. 2022

Int. J. Med. Sci.

View full text Add to dashboard Cite

show abstract

“…Serum collection: Serum samples were collected from rabbits in the sham operation and OA groups preoperatively as well as 0, 2, 4, 6, 8, 10, and 12 weeks (6 rabbits in each group) postoperatively. After the blood was centrifuged, the supernatant was collected to evaluate the serum levels of CTX‐II and IL‐1β.…”

Section: Methodsmentioning

confidence: 99%

Correlation Analysis of C‐terminal telopeptide of collagen type II and Interleukin‐1β for Early Diagnosis of Knee Osteoarthritis

Liu

Gao

Qin

et al. 2019

Orthopaedic Surgery

View full text Add to dashboard Cite

Objective To analyze the correlation between the Kellgren–Lawrence (K‐L) score of knee osteoarthritis (KOA) patients with different degrees and their urine concentration of C‐terminal telopeptide of collagen type II (CTX‐II) and interleukin‐1β (IL‐1β), and to further evaluate the diagnostic value of CTX‐II and IL‐1β during the pathological process by producing an experimental osteoarthritis (OA) model in rabbits. Methods From 1 January 2017 to 31 December 2018, a total of 34 subjects (7 mild, 9 moderate, 9 severe arthritis patients, and 9 healthy individuals) comprising 16 men and 18 women were included in this study. Patients were diagnosed according to the American College of Rheumatology (ACR) criteria. The urine of all subjects was collected to detect the concentration of CTX‐II and IL‐1β. The rabbits in the KOA group were subjected to protease (control group with saline) injection into the articular cavity of their right knees and immobilization with gypsum. We used radiological and histological examination to identify the KOA model. ELISA was applied to investigate the concentrations of CTX‐II and IL‐1β in urine and serum, and Spearman's rank correlation analysis was used to analyze the correlation. Results There was no significant difference in the mean ages and body mass index (BMI) between groups. The mean ages of mild, moderate, and severe arthritis patients and healthy individuals were 54.29 ± 5.76, 58.44 ± 6.44, 59.89 ± 6.75, and 56.67 ± 4.18 years, respectively. The mean BMI of mild, moderate, and severe arthritis patients and healthy individuals were 23.59 ± 1.56, 23.57 ± 2.06, 24.46 ± 1.64, and 23.42 ± 1.35 kg/m2, respectively. The Kellgren–Lawrence (K‐L) score was higher with the aggravation of KOA. The K‐L scores of mild, moderate, and severe KOA patients were 1.14 ± 0.38, 2.56 ± 0.53, and 3.63 ± 0.52, respectively. The KOA symptoms of patients became more severe, with not only increased K‐L scores but also elevated concentrations of CTX‐II and IL‐1β. Moreover, there was a positive correlation between CTX‐II and IL‐1β of all subjects (r = 0.974, P < 0.001), between K‐L score and urine concentration of CTX‐II (r = 0.900, P < 0.001), and between K‐L score and IL‐1β (r = 0.813, P < 0.001) of all subjects. Both were significantly increased in KOA group rabbits at all time points after surgery. The serum concentration of CTX‐II and IL‐1β was elevated as early as in the 2nd week (3.69 and 4.25 times) and reached a peak (5.41 and 7.23 times) in the 4th week after surgery. Then, until 12 weeks after surgery, the CTX‐II and IL‐1β concentrations in the KOA group were slightly reduced and remained around 4.5 and 6.3 times that in the control group. Moreover, there was a positive correlation between the serum concentration of IL‐1β and CTX‐II (r = 0.967, P < 0.001). Conclusion CTX‐II and IL‐1β, which were significantly increased during the process of KOA, can be used as biomolecular markers to provide guidelines for early diagnosis and treatment of KOA.

show abstract

“…One model that has also emerged recently is the tibial osteotomy in mice. In this model, the tibia is surgically adjusted to a varus or valgus alignment altering the mechanical loading on the medial or lateral side of the knee joint [ 67 ]. This model avoids intra-articular surgery which poses the risk of damage to the intra-articular tissue and joints.…”

Section: Secondary Osteoarthritis Animal Modelsmentioning

confidence: 99%

“…Although this is a surgical method of inducing OA, it may potentially be used to investigate primary OA. This model is analogous to human primary OA where misalignment of the joint in a varus or valgus angle leads to OA without any internal damage to the joint [ 67 ].…”

Section: Secondary Osteoarthritis Animal Modelsmentioning

confidence: 99%

Regenerative Engineering Animal Models for Knee Osteoarthritis

Esdaille

Ude

Laurencin

2021

Regen. Eng. Transl. Med.

View full text Add to dashboard Cite

Osteoarthritis (OA) of the knee is the most common synovial joint disorder worldwide, with a growing incidence due to increasing rates of obesity and an aging population. A significant amount of research is currently being conducted to further our understanding of the pathophysiology of knee osteoarthritis to design less invasive and more effective treatment options once conservative management has failed. Regenerative engineering techniques have shown promising preclinical results in treating OA due to their innovative approaches and have emerged as a popular area of study. To investigate these therapeutics, animal models of OA have been used in preclinical trials. There are various mechanisms by which OA can be induced in the knee/stifle of animals that are classified by the etiology of the OA that they are designed to recapitulate. Thus, it is essential to utilize the correct animal model in studies that are investigating regenerative engineering techniques for proper translation of efficacy into clinical trials. This review discusses the various animal models of OA that may be used in preclinical regenerative engineering trials and the corresponding classification system. Lay Summary Osteoarthritis (OA) of the knee is the most common synovial joint disease worldwide, with high rates of occurrence due to an increase in obesity and an aging population. A great deal of research is currently underway to further our understanding of the causes of osteoarthritis, to design more effective treatments. The emergence of regenerative engineering has provided physicians and investigators with unique opportunities to join ideas in tackling human diseases such as OA. Once the concept is proven to work, the initial procedure for the evaluation of a treatment solution begins with an animal model. Thus, it is essential to utilize a suitable animal model that reflects the particular ailment in regenerative engineering studies for proper translation to human patients as each model has associated advantages and disadvantages. There are various ways by which OA can occur in the knee joint, which are classified according to the particular cause of the OA. This review discusses the various animal models of OA that may be used in preclinical regenerative engineering investigations and the corresponding classification system.

show abstract

Tibial Osteotomy as a Mechanical Model of Primary Osteoarthritis in Rats

Cited by 8 publications

References 41 publications

Pathological, Morphometric and Correlation Analysis of the Modified Mankin Score, Tidemark Roughness and Calcified Cartilage Thickness in Rat Knee Osteoarthritis after Extracorporeal Shockwave Therapy

Pathological, Morphometric and Correlation Analysis of the Modified Mankin Score, Tidemark Roughness and Calcified Cartilage Thickness in Rat Knee Osteoarthritis after Extracorporeal Shockwave Therapy

Correlation Analysis of C‐terminal telopeptide of collagen type II and Interleukin‐1β for Early Diagnosis of Knee Osteoarthritis

Regenerative Engineering Animal Models for Knee Osteoarthritis

Contact Info

Product

Resources

About