Tid1 Negatively Regulates the Migratory Potential of Cancer Cells by Inhibiting the Production of Interleukin-8

Kim, Sung Woo; Hayashi, Masaaki; Lo, Jeng‐Fan; Fearns, Colleen; Xiang, Rong; Lazennec, Gwendal; Yang, Young Chul; Lee, Jiing Dwan

doi:10.1158/0008-5472.can-04-4422

Cited by 44 publications

(35 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3A). As recently reported, the expression of IL-8 was associated with enhanced migratory potential and invasiveness of metastatic breast cancer cells (33 shown in Fig. 3B, conditioned media from H 2 O 2 -treated cells induced migration of MCF-7 cells in a time-response manner, whereas pre-treatment with G. lucidum inhibits oxidative stress-induced migration in a dose-response manner.…”

Section: G Lucidum Suppresses Oxidative Stress-induced Secretion Of supporting

confidence: 84%

Inhibition of oxidative stress-induced invasiveness of cancer cells by Ganoderma lucidum is mediated through the suppression of interleukin-8 secretion

et al. 2006

View full text Add to dashboard Cite

Abstract. Epidemiological studies suggest that the intake of natural/nutrient products is inversely related to cancer risk. While oxidative stress, generating reactive oxygen species, has been linked to cancer initiation and progression, dietary antioxidants have reduced the risk of certain cancers. Experimental studies have demonstrated that antioxidants and phytochemicals could prevent cancer metastasis, and antioxidants were suggested as adjuvants in cancer therapy. Ganoderma lucidum is an Asian medicinal mushroom that has been used for the past two thousand years for the treatment of various diseases, including cancer. G. lucidum is currently popular as a dietary supplement in the form of tea, powder or extract. We have previously demonstrated that G. lucidum suppresses growth, angiogenesis and invasiveness of highly invasive and metastatic breast cancer cells. The present study was undertaken to evaluate the effect of G. lucidum on oxidative stress-induced metastatic behavior of poorly-invasive MCF-7 breast cancer cells. We show that G. lucidum inhibits oxidative stressinduced migration of MCF-7 cells by the down-regulation of MAPK signaling. G. lucidum suppressed oxidative stress stimulated phosphorylation of extracellular signal-regulated protein kinases (Erk1/2), which resulted in the down-regulation of expression of c-Fos, and in the inhibition of transcription factors AP-1 and NF-κB. The biological effect of G. lucidum on cell migration was mediated by the suppression of secretion of interleukin-8 from MCF-7 cells exposed to oxidative stress. In summary, our results suggest that G. lucidum inhibits the oxidative stress-induced invasive behavior of breast cancer cells by modulating Erk1/2 signaling and can be potentially considered as an antioxidant in adjuvant cancer therapy.

show abstract

Section: G Lucidum Suppresses Oxidative Stress-induced Secretion Of supporting

confidence: 84%

Inhibition of oxidative stress-induced invasiveness of cancer cells by Ganoderma lucidum is mediated through the suppression of interleukin-8 secretion

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Here, we selected IL-8 for further analysis among the predicted targets of miR-520b because it can modulate cancer cell migration and metastasis through several signaling pathways, such as phosphatidylinositol 3-kinase and Rho-GTPases (17,20,39). We confirmed the correlation between IL-8 expression and migration of breast cancer cells (Fig.…”

Section: Discussionsupporting

confidence: 54%

miR-520b Regulates Migration of Breast Cancer Cells by Targeting Hepatitis B X-interacting Protein and Interleukin-8

Hu¹,

Zhang²,

Cui³

et al. 2011

Journal of Biological Chemistry

102

View full text Add to dashboard Cite

MicroRNAs play important roles in tumor metastasis. Recently, we reported that the level of miR-520b is inversely related to the metastatic potential of breast cancer cells. In this study, we investigated the role of miR-520b in breast cancer cell migration. We found that miR-520b suppressed the migration of breast cancer cells with high metastatic potential, including MDA-MB-231 and LM-MCF-7 cells, although the inhibition of miR-520b enhanced the migration of low metastatic potential MCF-7 cells. We further discovered that miR-520b directly targets the 3-untranslated region (3UTR) of either hepatitis B X-interacting protein (HBXIP) or interleukin-8 (IL-8), which has been reported to contribute to cell migration. Surprisingly, tissue array assays showed that 75% (38:49) and 94% (36:38) of breast cancer tissues and metastatic lymph tissues, respectively, were positive for HBXIP expression. Moreover, overexpression of HBXIP was able to promote the migration of MCF-7 cells. Interestingly, HBXIP was able to regulate IL-8 transcription by NF-B, suggesting that the two target genes of miR-520b are functionally connected. In addition, we found that miR-520b could indirectly regulate IL-8 transcription by targeting HBXIP. Thus, we conclude that miR-520b is involved in regulating breast cancer cell migration by targeting HBXIP and IL-8 via a network in which HBXIP promotes migration by stimulating NF-B-mediated IL-8 expression. These studies point to HBXIP as a potential therapeutic target for breast cancer. MicroRNAs (miRNAs)3 are a class of small noncoding RNAs that act as post-transcriptional regulators of gene expression. It is currently estimated that the human genome may contain at least 700 miRNAs (1, 2). Emerging evidence has demonstrated that miRNAs can function as oncogenes or tumor suppressors involved in neoplasm development, progression, diagnosis, and prognostication (3, 4). It was recently reported that miRNAs are mutated or differentially expressed in breast cancer, with profound positive or negative effects on breast cancer cell migration. For instance, miR-10b is highly expressed in human and mouse metastatic breast cancer cell lines and positively regulates cell migration (5), and MiR-27b stimulates cell migration by targeting ST14 (6). Let-7g targets collagen type I ␣2 and inhibits cell migration in hepatocellular carcinoma (7). Although a large number of miRNAs have been identified to date, their roles in breast cancer cell migration and the underlying mechanisms of this regulation remain to be determined.Hepatitis B X-interacting protein (HBXIP) was originally identified by its interaction with the C terminus of the hepatitis B virus X protein (8). It forms a complex with survivin, leading to the suppression of apoptosis initiated through the mitochondrial cytochrome c pathway (9). HBXIP was shown to interact with the hSuv3 protein, which encodes an NTP-dependent DNA/RNA DEXH box helicase predominantly localized in mitochondria (10). Furthermore, HBXIP also plays a critical role in mitosis, serving ...

show abstract

“…Recently, increasing evidence has suggested that chemokines, such as IL8, S100A8, CXCL14, and CCL5, produced by stromal cells surrounding tumors are associated with tumor development and progression via cross-talk between tumor and stromal cells. IL8 has been shown to play important roles in angiogenesis and metastasis (38), and neutralization of IL8 by an anti-IL8 antibody has been shown to inhibit angiogenesis, tumor growth, and metastasis in malignant melanoma (39). One recent report found that S100A8 secreted by a distant primary tumor could attract Mac 1(+)-myeloid cells to the premetastatic lung, suggesting that S100A8 plays a role in long distance metastasis (40).…”

Section: Discussionmentioning

confidence: 99%

Fibroblast Growth Factor Receptor 2–Positive Fibroblasts Provide a Suitable Microenvironment for Tumor Development and Progression in Esophageal Carcinoma

Zhang

et al. 2009

Clinical Cancer Research

103

View full text Add to dashboard Cite

Purpose: Tumor fibroblasts (TF) have been suggested to play an essential role in the complex process of tumor-stroma interactions and tumorigenesis. The aim of the present study was to investigate the specific role of TF in the esophageal cancer microenvironment. Experimental Design: An Affymetrix expression microarray was used to compare gene expression profiles between six pairs of TFs and normal fibroblasts from esophageal squamous cell carcinoma (ESCC). Differentially expressed genes were identified, and a subset was evaluated by quantitative real-time PCR and immunohistochemistry. Results: About 43% (126 of 292) of known deregulated genes in TFs were associated with cell proliferation, extracellular matrix remodeling, and immune response. Up-regulation of fibroblast growth factor receptor 2 (FGFR2), which showed the most significant change, was detected in all six tested TFs compared with their paired normal fibroblasts. A further study found that FGFR2-positive fibroblasts were only observed inside the tumor tissues and not in tumor-surrounding stromal tissues, suggesting that FGFR2 could be used as a TF-specific marker in ESCC. Moreover, the conditioned medium from TFs was found to be able to promote ESCC tumor cell growth, migration, and invasion in vitro. Conclusions: Our study provides new candidate genes for the esophageal cancer microenvironment. Based on our results, we hypothesize that FGFR2(+)-TFs might provide cancer cells with a suitable microenvironment via secretion of proteins that could promote cancer development and progression through stimulation of cancer cell proliferation, induction of angiogenesis, inhibition of cell adhesion, enhancement of cell mobility, and promotion of the epithelial-mesenchymal transition.

show abstract

Tid1 Negatively Regulates the Migratory Potential of Cancer Cells by Inhibiting the Production of Interleukin-8

Cited by 44 publications

References 30 publications

Inhibition of oxidative stress-induced invasiveness of cancer cells by Ganoderma lucidum is mediated through the suppression of interleukin-8 secretion

Inhibition of oxidative stress-induced invasiveness of cancer cells by Ganoderma lucidum is mediated through the suppression of interleukin-8 secretion

miR-520b Regulates Migration of Breast Cancer Cells by Targeting Hepatitis B X-interacting Protein and Interleukin-8

Fibroblast Growth Factor Receptor 2–Positive Fibroblasts Provide a Suitable Microenvironment for Tumor Development and Progression in Esophageal Carcinoma

Contact Info

Product

Resources

About