Tilapia (Oreochromis aureus) Collagen for Medical Biomaterials

Valenzuela-Rojo, David R.; López‐Cervantes, Jaime; Sánchez‐Machado, Dalia I.

doi:10.5772/intechopen.77051

Cited by 18 publications

(13 citation statements)

References 70 publications

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“…FTIR analysis confirmed that the carbon rich layer we found with the EDS is composed by organic residues particularly the C-H stretch and v(C=O) peaks around 2931 and 1737 respectively (Fig. 5A), which are commonly found in collagen I (Belbachir et al 2009;Jeevithan et al 2014;Valenzuela-Rojo et al 2018) and keratin (Chandini et al 2017;Estévez-Martínez et al 2013); highly abundant proteins found in the scales and skin of fishes (Bhagwat & Dandge 2016;Elliott 2011). Amide A, I, II, and III, C-H stretch and v(C=O) peaks were clearly observed in the FTIR of the extant Orechromis sp.…”

Section: Sem-eds Resultssupporting

confidence: 66%

Peer Review #1 of "Exceptionally preserved ‘skin’ in an Early Cretaceous fish from Colombia (v0.1)"

Benton¹

2020

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Studies of soft tissue, cells and original biomolecular constituents preserved in fossil vertebrates have increased notoriously in recent years. Here we report preservation of 'skin' with chemical and molecular characterization from a three-dimensionally preserved caudal portion of an aspidorhynchid Cretaceous fish from the equatorial Barremian of Colombia increasing the number of localities for which exceptional preservation is known. We applied several analytical techniques including SEM-EDS, FTIR and ToF-SIMS to characterize the micromorphology and molecular and elemental composition of this fossil. Here, we show that the fossilized 'skin' exhibits similarities with those from extant fish including the wrinkles after suffering compression stress and flexibility, as well as architectural and tissue aspects of the two main layers (epidermis and dermis). This similarity extends also to molecular level, with the demonstrated preservation of potential residues of original proteins not consistent with bacterial source. Our results show a potential preservation mechanism where scales may have acted as an external barrier and together with an internal phosphate layer resulting from the degradation of the dermis itself creating an encapsulated environment for the integument.

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Section: Sem-eds Resultssupporting

confidence: 66%

Peer Review #1 of "Exceptionally preserved ‘skin’ in an Early Cretaceous fish from Colombia (v0.1)"

Benton¹

2020

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“…FTIR analysis confirmed that the carbon rich layer we found with the EDS is composed of, organic residues, particularly the C-H stretch and v(C=O) peaks around 2,931 and 1,737 respectively (Fig. 5A), which are commonly found in collagen I (Belbachir et al, 2009;Jeevithan et al, 2014;Valenzuela-Rojo, López-Cervantes & Sánchez-Machado, 2018) and keratin (Chandini et al, 2017;Estévez-Martínez et al, 2013); highly abundant proteins found in the scales and skin of fishes (Bhagwat & Dandge, 2016;Elliott, 2011). Amide A, I, II, and III, C-H stretch and v(C=O) peaks were clearly observed in the FTIR of the extant Orechromis sp.…”

Section: Systematic Paleontologysupporting

confidence: 66%

Exceptionally preserved ‘skin’ in an Early Cretaceous fish from Colombia

Alfonso-Rojas

Cadena

2020

PeerJ

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Studies of soft tissue, cells and original biomolecular constituents preserved in fossil vertebrates have increased greatly in recent years. Here we report preservation of ‘skin’ with chemical and molecular characterization from a three-dimensionally preserved caudal portion of an aspidorhynchid Cretaceous fish from the equatorial Barremian of Colombia, increasing the number of localities for which exceptional preservation is known. We applied several analytical techniques including SEM-EDS, FTIR and ToF-SIMS to characterize the micromorphology and molecular and elemental composition of this fossil. Here, we show that the fossilized ‘skin’ exhibits similarities with those from extant fish, including the wrinkles after suffering compression stress and flexibility, as well as architectural and tissue aspects of the two main layers (epidermis and dermis). This similarity extends also to the molecular level, with the demonstrated preservation of potential residues of original proteins not consistent with a bacterial source. Our results show a potential preservation mechanism where scales may have acted as an external barrier and together with an internal phosphate layer resulting from the degradation of the dermis itself creating an encapsulated environment for the integument.

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“…A higher Td indicates a stronger bonding within the collagen triple helix chains. Fish collagen is known to have lower Td than mammalian sources [ 31 ], so the increase of Td is desirable for most tissue engineering applications.…”

Section: Resultsmentioning

confidence: 99%

“…After that, it was rinsed thoroughly with deionized water. Fat contents were removed using ethanol: acetone 1:1 mixture as described by [ 31 ]. Afterward, the skins were exhaustively washed with deionized water to prepare for collagen extraction.…”

Section: Methodsmentioning

confidence: 99%

Mineralization of Phosphorylated Fish Skin Collagen/Mangosteen Scaffolds as Potential Materials for Bone Tissue Regeneration

et al. 2021

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In this study, a potential hard tissue substitute was mimicked using collagen/mangosteen porous scaffolds. Collagen was extracted from Tilapia fish skin and mangosteen from the waste peel of the respective fruit. Sodium trimetaphosphate was used for the phosphorylation of these scaffolds to improve the nucleation sites for the mineralization process. Phosphate groups were incorporated in the collagen structure as confirmed by their attenuated total reflection Fourier transform infrared (ATR-FTIR) bands. The phosphorylation and mangosteen addition increased the thermal stability of the collagen triple helix structure, as demonstrated by differential scanning calorimetry (DSC) and thermogravimetry (TGA) characterizations. Mineralization was successfully achieved, and the presence of calcium phosphate was visualized by scanning electron microscopy (SEM). Nevertheless, the porous structure was maintained, which is an essential characteristic for the desired application. The deposited mineral was amorphous calcium phosphate, as confirmed by energy dispersive X-ray spectroscopy (EDX) results.

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Tilapia (Oreochromis aureus) Collagen for Medical Biomaterials

Cited by 18 publications

References 70 publications

Peer Review #1 of "Exceptionally preserved ‘skin’ in an Early Cretaceous fish from Colombia (v0.1)"

Peer Review #1 of "Exceptionally preserved ‘skin’ in an Early Cretaceous fish from Colombia (v0.1)"

Exceptionally preserved ‘skin’ in an Early Cretaceous fish from Colombia

Mineralization of Phosphorylated Fish Skin Collagen/Mangosteen Scaffolds as Potential Materials for Bone Tissue Regeneration

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