Time-course and specificity of the arginine requirement for adenovirus biosynthesis

Bonïfas, V.

doi:10.1007/bf01245766

Cited by 14 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is normal if we consider that the feedings were done with MEM amino acids in which the highest amino acid concentration is arginine. Noteworthy, arginine had been demonstrated as absolutely necessary for adenovirus production (Bonifas, 1967;Clark Rouse and Schlesinger, 1967;Heilman and Rouse, 1980;Kumel and Hammer, 1979;Wigand and Kumel, 1978). Clark Rouse and Schlesinger, (1967) had proposed earlier that arginine played a specific role in a late synthetic step for virion maturation.…”

Section: Discussionmentioning

confidence: 97%

293SF Metabolic Flux Analysis during Cell Growth and Infection with an Adenoviral Vector

Nadeau

Jacob²,

Perrier

et al. 2000

Biotechnol. Prog.

View full text Add to dashboard Cite

Metabolic flux quantification of cell culture is becoming a crucial means to improve cell growth as well as protein and vector productions. The technique allows rapid determination of cell culture status, thus providing a tool for further feeding improvements. Herein, we report on key results of a metabolic investigation using 293 cells adapted to suspension and serum-free medium (293SF) during growth and infection with an adenoviral vector encoding the green fluorescence protein (GFP). The model developed contains 35 fluxes, which include the main fluxes of glycolysis, glutaminolysis, and amino acids pathways. It requires specific consumption and production rate measurements of amino acids, glucose, lactate, NH(3), and O(2), as well as DNA and total proteins biosynthesis rate measurements. Also, it was found that extracellular protein concentration measurement is important for flux calculation accuracy. With this model, we are able to describe the 293SF cell metabolism, grown under different culture conditions in a 3-L controlled bioreactor for batch and fed-batch with low glucose. The metabolism is also investigated during infection under two different feeding strategies: a fed-batch starting at the end of the growth phase and extending during infection without medium change and a fed-batch after infection following medium renewal. Differences in metabolism are observed between growth and infection, as well as between the different feeding strategies, thus providing a better understanding of the general metabolism.

show abstract

Section: Discussionmentioning

confidence: 97%

293SF Metabolic Flux Analysis during Cell Growth and Infection with an Adenoviral Vector

Nadeau

Jacob²,

Perrier

et al. 2000

Biotechnol. Prog.

View full text Add to dashboard Cite

show abstract

“…Indeed the block caused by arginine deficiency was prior to the formation of viral DNA. In most of the DNA viruses so far studied for effect of arginine deprivation, that is herpes simplex (7, 10, t 1, 25, 41, 42), adenovirus (8,14,17,36,37,38,39), polyoma (44,45) and 8V40 (16), the block in the cycle has been found to oeeur in the second stage of development, and more explicitly after the produetion of virus speeifie DNA. However a requirement for 0.015 mM arginine existed in the early stages of vaecinia development (2).…”

Section: Discussionmentioning

confidence: 97%

The effect of arginine deprivation on the cytopathogenic effect and replication of human cytomegalovirus

Garnett

1975

Archives of Virology

View full text Add to dashboard Cite

SummaryArginine is necessary for the development of the cytopathogenic effect of human cytomegalovirus in human embryonic fibroblasts. It is also required, though in greater concentrations, for the production of infective virions, the reqnirement being at an early stage of replication. Inhibitor studies suggested that this block in replication caused by arginine deficiency was prior to the formation of viral DNA. Withdrawal of arginine from the medium 24 or 48 hours after infection resulted in a decline in virus production indicating that the continued presence of the amino acid is necessary for constant virus production. Infected cultures deprived of arginine could be stimulated to produce eytopathie effects and infective virions by replacement of the amino acid even eight days after inoculation, demonstrating that the information for cytomegalovirus replication remains intact within the cell. This establishment of latency in vitro may be related to the ability of the virus to establish a similar state in vivo.

show abstract

“…The presence of arginine and histidine in the extracellular medium was found to be essential for the replication of animal virions such as herpes simplex and adenovirus, whereas the omission of lysine did not appreciably affect virion yields (2,3,11). Furthermore, in such systems of argininedeficiency it was found that virion-specific early and structural proteins and viral deoxyribonucleic acid (DNA) were synthesized (1, 6).…”

Section: Discussionmentioning

confidence: 99%

“…The requirement for the amino acid arginine in the replication of several animal virions has been reported (2,11). For both the adenovirus and the herpes simplex virus, arginine was found to be essential for viral maturation (1,6).…”

mentioning

confidence: 99%

Role of Lysine in the Replication of Reovirus

Loh

Oie

1969

J Virol

View full text Add to dashboard Cite

Lysine is essential for the replication of infectious reovirus. Omission of lysine from the extracellular medium not only permitted the continued synthesis of structural viral proteins and viral double-stranded ribonucleic acid (RNA), but also caused an enhanced formation of viral structures which were separable by isopycnic sedimentation of CsCl into a top band consisting of empty particles with a buoyant density of 1.29 g/cm 3 and essentially free of viral RNA, and two lower bands which were difficult to resolve and had an average buoyant density of 1.37 g/cm 3 . The lower bands contained most of the viral nucleic acid. The above effects were reversed when lysine was restored early after infection. In contrast, a single band with a buoyant density of 1.38 g/cm 3 was obtained from lysine-plus infected cells.

show abstract

Time-course and specificity of the arginine requirement for adenovirus biosynthesis

Cited by 14 publications

References 6 publications

293SF Metabolic Flux Analysis during Cell Growth and Infection with an Adenoviral Vector

293SF Metabolic Flux Analysis during Cell Growth and Infection with an Adenoviral Vector

The effect of arginine deprivation on the cytopathogenic effect and replication of human cytomegalovirus

Role of Lysine in the Replication of Reovirus

Contact Info

Product

Resources

About