Time course of cardiac structural and functional changes in babies in the first year of life who have sustained transient myocardial ischemia in the early neonatal period

Лебеденко, А. А.; Тараканова, Т. Д.; Козырева, Т. Б.; Левчин, А. М.; Аверкина, Л. А.

doi:10.21508/1027-4065-2017-62-1-53-59

Cited by 4 publications

(1 citation statement)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Botting et al (2012) [3]. Myocardial damage in IUH conditions has significant clinical consequences [4], which can occur in the long run rather than at early stages of postnatal ontogenesis alone. D. Barker (2003) described the phenomenon of the so-called "fetal programming" in individuals born with an IUH-caused low body mass (suggesting an early gestational age) in the form of arterial hypertension and coronary heart disease in adulthood [5].…”

Section: Introductionmentioning

confidence: 99%

Untitled

2019

Russ Open Med J

View full text Add to dashboard Cite

Objective-Our study aimed to evaluate the possibility of correcting cardiac consequences of intrauterine hypoxia (IUH) by injecting leu-enkephalin analog, lacking affinity for opiate receptors, in the early postnatal period. Material and Methods-To model IUH, we placed pregnant Wistar rats in a hypobaric chamber with an oxygen partial pressure of 52 mmHg. The procedure was repeated for 4 h daily over the 15 th-19 th days of gestation. From the 2 nd through the 6 th days of their lives, the offspring were injected intraperitoneally with non-opiate leu-enkephalin analog at a dose of 100 μg/kg (NALE: Phe-D-Ala-Gly-Phe-Leu-Arg). This analog did not have affinity for opiate receptors. The 7-and 60-day old offspring of female rats subjected to IUH were investigated. The control group included the descendants of intact animals. We investigated gravimetric indicators, DNA-synthetic activity of cardiomyocytes (CMC) by tritium-labeled thymidine autoradiography method, the size of the CMC nuclei, as well as size and amount of nucleoli in the CMC nuclei. The activity of free radical oxidation was evaluated in cardiac homogenates by chemiluminescence. Results-In 7-day old rats subjected to IUH vs. control animals, we observed decreases in body mass by 32.6%, in heart mass by 27.3%; in the proportion of 3 Н-thymidine labeled CMC nuclei by 32.7% in the left ventricle and by 30.4% in the right ventricle; in the number of nucleoli in the CMC nuclei (in the left ventricle: control-2.384±0.027, IUH-2.282±0.027*, p<0.05; in the right ventricle: control-2.409±0.038; IUH-2.240±0.012*, p<0, 05). Increase in CML indices of cardiac homogenates was revealed, indicating the activation of free radical oxidation. In 7-day old rats subjected to IUH and administration of the NALE peptide from the 2 nd through the 6 th days of their lives, the proportion of 3 H-thymidine labeled nuclei in the CMC did not differ from the control (in the left ventricle: control-12.79±0.89%, IUH + NALE-10.98±0.95%, p>0.05; in the right ventricle: control-11.61±0.78%; IUH + NALE-11.26±0.58%, p>0.05). The number of nucleoli in the CMC nuclei of the left and right ventricles in the heart of 7-day old animals in the IUH + NALE group did not differ from the control too. The CML indices of heart homogenates in the IUH + NALE group were significantly lower than those in the IUH group. In 60-day old male rats exposed to IUH, there was a decrease in heart mass by 18.5%, sizes of CMC nuclei by 7.5% and 16.1% in the left and right ventricles, respectively, and in the total nucleoli area in the CMC nuclei of the left ventricle (control-3.953±0.085; IUH-3.372±0.078*; p<0.05). In 60-day old male rats subjected to IUH and injections of the NALE peptide from the 2 nd to the 6 th days of their lives, heart mass (control-692.73±26.81 mg; IUH + NALE-631.0±29.79 mg; p>0.05) and the size of the CMC nuclei of the right ventricle (control-54.25±0.84; IUH + NALE-55.24±0.94; p>0.05) did not differ significantly from the control. The size of the nuclei, the number and size of the nucleoli ...

show abstract

Section: Introductionmentioning

confidence: 99%

Untitled

2019

Russ Open Med J

View full text Add to dashboard Cite

show abstract

The influence of non-opiate analogue of leu-enkephalin to the cardiac consequences of intrauterine hypoxia of albino rats

et al. 2019

View full text Add to dashboard Cite

Objective ― Our study aimed to evaluate the possibility of correcting cardiac consequences of intrauterine hypoxia (IUH) by injecting leu-enkephalin analog, lacking affinity for opiate receptors, in the early postnatal period. Material and Methods ― To model IUH, we placed pregnant Wistar rats in a hypobaric chamber with an oxygen partial pressure of 52 mmHg. The procedure was repeated for 4 h daily over the 15th-19th days of gestation. From the 2nd through the 6th days of their lives, the offspring were injected intraperitoneally with non-opiate leu-enkephalin analog at a dose of 100 μg/kg (NALE: Phe-D-Ala-Gly-Phe-Leu-Arg). This analog did not have affinity for opiate receptors. The 7- and 60-day old offspring of female rats subjected to IUH were investigated. The control group included the descendants of intact animals. We investigated gravimetric indicators, DNA-synthetic activity of cardiomyocytes (CMC) by tritium-labeled thymidine autoradiography method, the size of the CMC nuclei, as well as size and amount of nucleoli in the CMC nuclei. The activity of free radical oxidation was evaluated in cardiac homogenates by chemiluminescence. Results ― In 7-day old rats subjected to IUH vs. control animals, we observed decreases in body mass by 32.6%, in heart mass by 27.3%; in the proportion of 3Н-thymidine labeled CMC nuclei by 32.7% in the left ventricle and by 30.4% in the right ventricle; in the number of nucleoli in the CMC nuclei (in the left ventricle: control – 2.384±0.027, IUH – 2.282±0.027*, p<0.05; in the right ventricle: control – 2.409±0.038; IUH – 2.240±0.012*, p<0, 05). Increase in CML indices of cardiac homogenates was revealed, indicating the activation of free radical oxidation. In 7-day old rats subjected to IUH and administration of the NALE peptide from the 2nd through the 6th days of their lives, the proportion of 3H-thymidine labeled nuclei in the CMC did not differ from the control (in the left ventricle: control – 12.79±0.89%, IUH + NALE – 10.98±0.95%, p>0.05; in the right ventricle: control – 11.61±0.78%; IUH + NALE – 11.26±0.58%, p>0.05). The number of nucleoli in the CMC nuclei of the left and right ventricles in the heart of 7-day old animals in the IUH + NALE group did not differ from the control too. The CML indices of heart homogenates in the IUH + NALE group were significantly lower than those in the IUH group. In 60-day old male rats exposed to IUH, there was a decrease in heart mass by 18.5%, sizes of CMC nuclei by 7.5% and 16.1% in the left and right ventricles, respectively, and in the total nucleoli area in the CMC nuclei of the left ventricle (control – 3.953±0.085; IUH – 3.372±0.078*; p<0.05). In 60-day old male rats subjected to IUH and injections of the NALE peptide from the 2nd to the 6th days of their lives, heart mass (control – 692.73±26.81 mg; IUH + NALE – 631.0±29.79 mg; p>0.05) and the size of the CMC nuclei of the right ventricle (control – 54.25±0.84; IUH + NALE – 55.24±0.94; p>0.05) did not differ significantly from the control. The size of the nuclei, the number and size of the nucleoli in the CMC of the left ventricle, as well as the area of the nucleoli in the CMC of the right ventricle in 60-day old male rats of the IUH + NALE group significantly exceeded control group values. Conclusion ― Administration of the NALE peptide to albino rats subjected to IUH normalized DNA-synthetic activity and the number of nucleoli in the nuclei of CMC in 7-day old animals, and also reduced the severity of oxidative stress in the heart tissue. In 60-day old albino male rats exposed to IUH, injecting NALE from the 2nd to the 6th days of their lives eliminated declines in heart mass and sizes of the CMC nuclei and nucleoli, and also led to an increase in the values of the nucleus-and-nucleolus complex indices compared with the control.

show abstract

Peculiarities of hemodynamic status of healthy newborns in early neonatal period

Хубулава

Марченко

Naumov

et al. 2019

Ross. vestn. perinatol. pediatr.

View full text Add to dashboard Cite

Марченко Сергей Павлович-д.м.н., проф. кафедры сердечно-сосудистой хирургии Санкт-Петербургского государственного педиатрического медицинского университета, ORCID: 0000-0003-4785-4532 Наумов Алексей Борисович-к.м.н., доцент кафедры анестезиологииреаниматологии и неотложной педиатрии факультета послевузовского и дополнительного профессионального образования Санкт-Петербургского государственного педиатрического медицинского университета, ORCID: 0000-0003-0323-0162 Чупаева Ольга Юрьевна-врач анестезиолог-реаниматолог отделения анестезиологии-реанимации для детей с кардиохирургической патологией Санкт-Петербургского государственного педиатрического медицинского университета, ORCID: 0000-0003-4204-6915

show abstract

Time course of cardiac structural and functional changes in babies in the first year of life who have sustained transient myocardial ischemia in the early neonatal period

Cited by 4 publications

References 2 publications

Untitled

Untitled

The influence of non-opiate analogue of leu-enkephalin to the cardiac consequences of intrauterine hypoxia of albino rats

Peculiarities of hemodynamic status of healthy newborns in early neonatal period

Contact Info

Product

Resources

About