Time course of in vitro expression of NADPH-diaphorase in cultured rat brain neurons: comparison with in vivo expression

Uehara-Kunugi, Yukiko; Terai, Kazuhiro; Taniguchi, Takashi; Tooyama, Ikuo; Kimura, Hiroshi

doi:10.1016/0165-3806(91)90095-z

Cited by 23 publications

(4 citation statements)

References 17 publications

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“…The migration appears to be guided in movement by the Bergmann's glial fibers (Reinis and Goldman 1980). There were no NADPH-d-positive neurons detected in the cerebellum of the embryo, but substantial expression was observed on P14, in agreement with a report in vivo and in vitro (Uehara-Kunugi et al 1991). The NO/cGMP system may influence cerebellar development in the postnatal stage or later in locomotive adjustments in the mature brain.…”

Section: Cerebellumsupporting

confidence: 89%

Ontogeny of NADPH-diaphorase in rat forebrain and midbrain

Iwase

Takemura

Shimada

et al. 1998

Anatomy and Embryology

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This study characterizes the developmental expression of NADPH-diaphorase from embryo to adulthood in the forebrain, midbrain and cerebellum of rat brain via histochemical staining. On embryonic day 12 no neurons stained. Labeling was observed in certain nuclei from E15 through the postnatal period to adulthood. Labeling in neurons increased or maintained a constant level with increased age. The embryo demonstrated substantial labeling in neurons of the caudate putamen, bed nucleus of the stria terminalis, preoptic area, lateral hypothalamic area, paraventricular thalamic nucleus, ventromedial hypothalamic nucleus, magnocellular nucleus posterior commissure, and periaqueductal central gray. Additional neuronal labeling was observed postnatally in the olfactory bulb, cerebral cortex, amygdala, various nuclei of the thalamus, interpeduncular nucleus, linear nucleus of the raphe, pretectal area and superior colliculus. In the cerebellum, labeling appeared only after P14 in cells of the molecular cell layer and granular cell layer. The sizes of labeled neurons developed significantly from P4 to P14 in several nuclei. The distinctive temporal and spatial expression pattern of NADPH-diaphorase implies that the NO/cGMP system may play an important role in physiological and developmental functions.

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Section: Cerebellumsupporting

confidence: 89%

Ontogeny of NADPH-diaphorase in rat forebrain and midbrain

Iwase

Takemura

Shimada

et al. 1998

Anatomy and Embryology

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“…Several studies have confirmed that the distribution pattern for NOS in fetal and postnatal brain [41] obtained by biochemical methods is consistent with that in histochemical studies [42][43][44][45][46], suggesting that NO has a role in the functional differentiation of the brain. NO appears to be involved in the removal of transient retinotectal projections, since the blockade of NOS activity results in the maintenance of such connections [47].…”

Section: Posterior Pituitarysupporting

confidence: 51%

“…Cramer et al reported that NADPH-d activity reaches a peak at about the fourth postnatal week, and then declines by the sixth postnatal week [48], and suggested that this transient expression of NADPH-d may be involved in the development of mature connections within the ferret lateral geniculate nucleus. In cultured neurons, NADPH-d was found to prolong the viability of NADPH-d positive neurons [45]. Tanaka et al reported that NV nitro-L-arginine, an inhibitor of NOS, inhibited granule cell migration, resulting in the cessation of granule cell proliferation and the differentiation of Bergman glial in cerebellar slice cultures [49].…”

Section: Posterior Pituitarymentioning

confidence: 99%

Postnatal Development of NADPH-Diaphorase Activity in the Rat. The Role of Nitric Oxide in the Ontogeny of Arginine Vasopression and Oxytocin.

et al. 2000

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Abstract.To clarify the role of nitric oxide (NO) in the ontogeny of arginine vasopressin (AVP) and oxytocin (OXT) neurons in the hypothalamo-neurohypophysial system (HNS), we observed the coexpression pattern of NADPHdiaphorase (NADPH-d) activity and AVP-or OXT-immunoreactivity (IR) in the rat hypothalamus and posterior pituitary during the postnatal period. The enzymatic activity of NADPH-d was observed in the supraoptic nucleus (SON), paraventricular nucleus (PVN), median eminence (ME) and posterior pituitary throughout the postnatal development. AVP-containing neurons were clearly observed from postnatal day 1 in both the SON and PVN, while OXT-containing neurons were recognized from postnatal day 14. The coexistence of NADPH-d and AVP or OXT was detected in the SON from postnatal day 14. At postnatal day 21, the coexpression pattern was approximately the same as that of the SON and PVN in adult rats. Our findings indicated that the expression of NADPH-d and OXT was observed from almost the same postnatal period in both the SON and PVN. In addition, the pattern of increased numbers of NADPH-d positive fibers was similar to that of OXT-immunoreactive fibers in both the inner layer of the ME and the posterior pituitary.A good correlation was thus obtained between OXT expression and NADPH-d activity in the HNS during postnatal development.The present study suggests that NO is more closely involved in the expression and regulation of secretion of OXT than AVP.

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“…While type I nitrinergic neurons appear relatively early in the developing cortex of non-primate mammals (Bredt and Snyder, 1994;Derer and Derer, 1993;Iwase et al, 1998;Northington et al, 1996;Riche et al, 1995;Terada et al, 1996;Uehara-Kunugi et al, 1991;Yan et al, 1994) and are present in the developing human cortex already during the first half of gestation (Yan and Ribak, 1997;Yan et al, 1996a), type II nitrinergic neurons in the primate cortex appear late, during the last weeks of gestation (Yan and Ribak, 1997;Yan et al, 1996a).…”

Section: Expression Of Nadph-d Activity and Nos-proteins In Cortical Layers II To Vi And In The Developing Cortical Platementioning

confidence: 99%

Nitrinergic neurons in the developing and adult human telencephalon: Transient and permanent patterns of expression in comparison to other mammals

Judaš

Šestan

Kostović

1999

Microsc. Res. Tech.

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A subpopulation of cerebral cortical neurons constitutively express nitric oxide synthase (NOS) and, upon demand, produce a novel messenger molecule nitric oxide (NO) with a variety of proposed roles in the developing, adult, and diseased brain. With respect to the intensity of their histochemical (NADPH-diaphorase histochemistry) and immunocytochemical (nNOS and eNOS immunocytochemistry) staining, these nitrinergic neurons are generally divided in type I and type II cells. Type I cells are usually large, intensely stained interneurons, scattered throughout all cortical layers; they frequently co-express GABA, neuropeptide Y, and somatostatin, but rarely contain calcium-binding proteins. Type II cells are small and lightly to moderately stained, about 20-fold more numerous than type I cells, located exclusively in supragranular layers, and found almost exclusively in the primate and human brain. In the developing cerebral cortex, nitrinergic neurons are among the earliest differentiating neurons, mostly because the dominant population of prenatal nitrinergic neurons are specific fetal subplate and Cajal-Retzius cells, which are the earliest generated neurons of the cortical anlage. However, at least in the human brain, a subpopulation of principal (pyramidal) cortical neurons transiently express NOS proteins in a regionally specific manner. In fact, transient overexpression of NOS-activity is a well-documented phenomenon in the developing mammalian cerebral cortex, suggesting that nitric oxide plays a significant role in the establishment and refinement of the cortical synaptic circuitry. Nitrinergic neurons are also present in human fetal basal forebrain and basal ganglia from 15 weeks of gestation onwards, thus being among the first chemically differentiated neurons within these brain regions. Finally, a subpopulation of human dorsal pallidal neurons transiently express NADPHdiaphorase activity during midgestation.

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Time course of in vitro expression of NADPH-diaphorase in cultured rat brain neurons: comparison with in vivo expression

Cited by 23 publications

References 17 publications

Ontogeny of NADPH-diaphorase in rat forebrain and midbrain

Ontogeny of NADPH-diaphorase in rat forebrain and midbrain

Postnatal Development of NADPH-Diaphorase Activity in the Rat. The Role of Nitric Oxide in the Ontogeny of Arginine Vasopression and Oxytocin.

Nitrinergic neurons in the developing and adult human telencephalon: Transient and permanent patterns of expression in comparison to other mammals

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