Time-Course Studies in Indole Alkaloid Accumulation and Changes in Tryptophan Decarboxylase and Strictosidine Synthase Activities: A Comparison in Three Strains of<i>Catharanthus roseus</i>Cells

Doireau, Pierre; Meriollon, J. M.; Guillot, A.; Rideau, Marc; Chénieux, Jean-Claude; Brillard, Michèle

doi:10.1055/s-2006-962739

Cited by 19 publications

(6 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, ajmalicine production varied in the different cultures. Such a lack of correlation between SSS activity measured in vitro and the capacity of alkaloid accumulation by the cells was also reported by Doireau et al (1987).…”

Section: Discussionsupporting

confidence: 55%

“…Their activities declined during the growth phase and increased continuously after reaching the stationary phase of growth (day 5). Doireau et al (1987) and Stevens et al (1992) observed the same negative relationship between growth and SSS activity in different cell lines of C. r o s e u s . In all culture conditions examined, SSS reached similar maximum activity levels.…”

Section: Discussionmentioning

confidence: 52%

See 1 more Smart Citation

Induction of ajmalicine formation and related enzyme activities in Catharanthus roseus cells: effect of inoculum density

Moreno

Schlatmann

Heijden

et al. 1993

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

In Catharanthus roseus cell cultures the time courses of four enzyme activities, tryptophan decarboxylase (TDC), strictosidine synthase (SSS), geraniol-10-hydroxylase (G10H) and anthranilate synthase (AS), and alkaloid accumulation were compared under two different culture conditions (low-inoculum density and high-inoculum density on induction medium) and a control on growth medium. In growth medium a transient increase in TDC activity was first observed after which G10H reached its maximum activity; only tryptamine accumulated, no ajmalicine could be detected. Apparently, a concerted induction of enzyme activities is required for ajmalicine formation. Cells inoculated in induction medium showed such a concerted induction of AS, TDC and G10H activities. After 30 days the low-density culture had accumulated six times more ajmalicine (in mumoles/g) than the high-density culture. Thus, increase in biomass concentration (high-density cultures) did not enhance the total alkaloid production. The major differences observed in enzyme levels between high- and low-density cultures were in the AS and TDC activities, which were two to three times higher in the low-density culture, indicating that there is a positive correlation between ajmalicine formation and AS and TDC activities.

show abstract

Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 52%

Induction of ajmalicine formation and related enzyme activities in Catharanthus roseus cells: effect of inoculum density

Moreno

Schlatmann

Heijden

et al. 1993

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…The messenger RNA for STR presents a region that encodes for a signal peptide of 31 amino acids that directs it to the vacuole (Pasquali et al 1992), which supports diverse tests that suggesting that STR is a vacuolar enzyme (McKnight et al 1991;Stevens et al 1993). Doireau et al (1987), and Stevens et al (1992) have measured the activity of TDC and STR, as well as the concentrations of tryptamine in suspension cultures of C. roseus and other species. These authors observed a tryptamine accumulation in spite of having a high activity of STR.…”

Section: Biosynthesis Of the Tiassupporting

confidence: 57%

“…The addition of geraniol, 10-hydroxygeraniol and secologanine, precursors of the terpenic pathway, increases the alkaloid content, specially tabersonine, in cellular suspensions, which seems to suggest that the production of alkaloids can be limited by one or more steps of the terpenic pathway (Merillon et al 1986;Schiel et al 1987;Doireau et al 1987;Facchini and DiCosmo 1991;Shanks et al 1998;Morgan and Shanks 2000). This conclusion cannot be generalized since contradictory evidence exists, such as the increase in the alkaloid production by means of the tryptamine addition to the culture medium (Zenk and Deus 1982).…”

Section: Regulationmentioning

confidence: 99%

Catharanthus biosynthetic enzymes: the road ahead

2007

View full text Add to dashboard Cite

Alkaloids are one of the most diverse groups of secondary metabolites found in living organisms. The most economically important alkaloids are the bisindole vinblastine, and vincristine. Unraveling the complexity of the genetic, catalytic and transport processes of monoterpene indole alkaloids biosynthesis is one of the most stimulating intellectual challenges in the plant secondary metabolism field. More than 50 metabolic steps are required to synthesize the most important alkaloids in Catharanthus roseus. Until now about only 20 of the 50 enzymes required for their biosynthesis have been determined and characterized. Hence, there are still a number of important enzymes that need to be characterized, beginning with the isolation and cloning of genes. It is also of fundamental importance to elucidate the regulatory aspects of their biosynthesis, both at the cellular and the molecular level, in order to address the question of their function in the plants that are producing them. In this review, we present an analysis of the state of the art related to the biosynthesis of the monoterpene indole alkaloids.

show abstract

“…Ajmalicine and serpentine come from the same side branch of the main pathway leading to vindoline, catharanthine and the dimeric antineoplastic compounds, vincristine and vinblastine (Noe et al, 1984;Merillon et al, 1986;Doireau et al, 1987). Ajmalicine is converted into serpentine through the activity of vacuolar basic peroxidases (Blom et al, 1991) and this conversion seems to be light-dependent (Knobloch et al, 1982).…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and alkaloid production in Catharanthus roseus, C. trichophyllus and their hybrids

Sevestre-Rigouzzo¹,

Nef-Campa

Ghesquiere³

et al. 1992

Euphytica

View full text Add to dashboard Cite

Inter and intraspecific variation was analyzed in two Catharanthus species with regard to isozyme polymorphism and indole alkaloid content in roots and leaves. No significant differences in alkaloid production were observed in three groups of C. roseus plants individualized for their flower color. Conversely, comparisons between C. trichophyllus and C. roseus, showed large differences of alkaloid profiles in both roots and leaves. Specific isozyme markers on four presumed loci were found allowing us to establish that natural hybridizations could occur between the two species when grown together. Experimental hybridizations confirmed that introgressions were feasible but suggested that a reproductive barrier was acting and involved interspecific incompatibility. The identification and assay of the main alkaloid compounds in natural interspecific hybrids displayed such a high hybrid vigor that interspecific hybridization may present a new and successful way of improving alkaloid production in Catharanthus species.

show abstract

Time-Course Studies in Indole Alkaloid Accumulation and Changes in Tryptophan Decarboxylase and Strictosidine Synthase Activities: A Comparison in Three Strains ofCatharanthus roseusCells

Cited by 19 publications

References 8 publications

Induction of ajmalicine formation and related enzyme activities in Catharanthus roseus cells: effect of inoculum density

Induction of ajmalicine formation and related enzyme activities in Catharanthus roseus cells: effect of inoculum density

Catharanthus biosynthetic enzymes: the road ahead

Genetic diversity and alkaloid production in Catharanthus roseus, C. trichophyllus and their hybrids

Contact Info

Product

Resources

About