Time-Course Studies on the Use of Loganin by Catharanthus roseus Cells Cultured in vitro

Naudascher, F.; Doireau, Pierre; Guillot, A.; Thiersault, Martine

doi:10.1016/s0176-1617(89)80134-8

Cited by 11 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The uptake kinetics for exogenous loganin added to the culture medium is dependent on the culture age (Naudascher et al 1989b). The loganin is completely taken up within 4 h in growth-phase cultures, whereas in stationary-phase cultures 40% of the amount added was still present in the culture medium.…”

Section: Additions To the Culture Medium With The Aim Of Increasing Ymentioning

confidence: 99%

Cell and tissue cultures of Catharanthus roseus: A literature survey

Moreno

Heijden

Verpoorte

1995

Plant Cell Tiss Organ Cult

217

100

View full text Add to dashboard Cite

The literature concerning the regulation and the biosynthesis of secondary metabolites in cell and tissue cultures of Catharanthus roseus is reviewed. The aim of this review is to summarise the progress achieved since the previous review of this subject from 1988 to December 1993. Several factors influencing the production of indole alkaloids are discussed. Special attention is given to large-scale cultivation methods. Some economic considerations on the production of ajmalicine are also discussed. ranth~ ro~eu~ cited in ~ C~c~ Abs~acts ~tween 1950 ~d 1993.most valuable components, the dimeric alkaloids vincristine and vinblastine, have so far only been detected in callus or organ cultures of C. roseus (Miura & Hirata 1982; Hoffmann et al. 1982;Miura et al. 1988).

show abstract

Section: Additions To the Culture Medium With The Aim Of Increasing Ymentioning

confidence: 99%

Cell and tissue cultures of Catharanthus roseus: A literature survey

Moreno

Heijden

Verpoorte

1995

Plant Cell Tiss Organ Cult

217

100

View full text Add to dashboard Cite

show abstract

“…Whereas the easiest application of secondary metabolism in plant cells is the bioconversion of chemicals with intact cells, this is often inadequate because of the relatively low activity in intact/cultured plant cells and the low recovery of metabolites due to complicated compartmentation in plant cells as well as uncontrolled catabolic reactions. 2,3) To overcome the problems encountered in using intact plant cells, purified biosynthetic enzymes have been used. For example, Amann and Zenk reported that immobilized (S)-tetrahydroprotoberberine oxidase was useful for the stereospecific oxidation of (S)-norreticuline.…”

mentioning

confidence: 99%

In VivoBioconversion of Tetrahydroisoquinoline by Recombinant CoclaurineN-Methyltransferase

Morishige

Choi

Sato

2004

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

An assay for secologanin in plant tissues based on enzymatic conversion into strictosidine

et al. 1998

View full text Add to dashboard Cite

The secoiridoid glucoside secologanin is the terpenoid building block in the biosynthesis of terpenoid indole alkaloids. A method for its determination in plant tissues and in cell suspension cultures has been developed. This assay is based on the condensation of secologanin with tryptamine, yielding strictosidine, in a reaction catalysed by the enzyme strictosidine synthase (STR; E.C. 4.3.3.2). Subsequently, the formation of strictosidine is quantified by high performance liquid chromatography (HPLC). STR was isolated from transgenic Nicotiana tabacum cells expressing a cDNA-derived gene coding for STR from Catharanthus roseus. The high specificity of STR for secologanin, in combination with a sensitive and selective HPLC system, allows a simple extraction of secologanin from plant tissue. The detection limit of this method is 15 ng secologanin. Using this assay, secologanin contents were determined in tissues of various plant species; Lonicera xylosteum hairy roots were found to contain 1% of secologanin on a dry weight basis.

show abstract

Time-Course Studies on the Use of Loganin by Catharanthus roseus Cells Cultured in vitro

Cited by 11 publications

References 15 publications

Cell and tissue cultures of Catharanthus roseus: A literature survey

Cell and tissue cultures of Catharanthus roseus: A literature survey

In VivoBioconversion of Tetrahydroisoquinoline by Recombinant CoclaurineN-Methyltransferase

An assay for secologanin in plant tissues based on enzymatic conversion into strictosidine

Contact Info

Product

Resources

About