Time dependent changes in aortic tissue during cold storage in physiological solution

Abstract

Background Stored vascular tissues are employed in biomedical research for studies in imaging, in biomechanics, and/or assessing vessel diseases. In the present study, The stability of aortic tissue in phosphate buffer saline (PBS) at 4°C was monitored over a course of 10 days as determined by the rate of glucose permeation measured by Optical Coherence Tomography (OCT) and validated by histology. Methods and Results The initial mean permeability through fresh porcine aorta was (2.32 ± 0.46) × 10−5 cm/s (n = 5); after maintaining the tissue at 4°C for 10 days, the mean rate was (7.37 ± 0.41) × 10−5 cm/s (n = 4), an increase of nearly 300%. A z-test verified that a significant change in the permeability rate (p<0.05) had occurred after 4 days of 4 °C storage. Histology was used to quantify changes in tissue pore area. The increase in average pore area paralleled the increase in permeability rate over 10 days. Conclusions These results suggest that (1) the structural integrity of aortic tissue at 4°C is retained for at least the first three days after resection, and (2) OCT is a powerful technology well suited for evaluating tissue structural integrity over time. General Significance Functional OCT imaging provides for a noninvasive and quantitative technique in determining the structural integrity of aortic tissue stored at 4°C. This modality may be used for assessing the efficacy of other preservation techniques.

Recent progress in tissue optical clearing

“…Many new original studies are elaborated using TOC in combination with laser speckle contrast imaging (LSCI) , optical coherence tomography (OCT) , microscopy imaging , ultramicroscopy , etc., which have demonstrated a great power not only for tissue structural and functional imaging with higher resolution , but also for optical imaging and diagnosis in vivo . In addition, when the OCT technique is applied to realize the time‐ and depth‐resolved monitoring of OCAs diffusion, it might be possible to distinguish between normal and pathological tissues .…”

Quantitative assessment of hyaline cartilage elasticity during optical clearing using optical coherence elastography

“…In OCT, this can be quantified easily by measuring the optical attenuation [2,4,6,7,9,10,20,21,[27][28][29][30][31][32][33]. However, in this study, the constant change of the fluid volume during the clearing process resulted in dynamic defocusing of the OCT signal and, thus, affecting the values of the OCT signal slope.…”

The Digram I Ching Genetic Code Compresses the Genetic Code into 24 Compatible Main Codons

“…consistent with known vasoactive responses.”, “.to high-throughput and high-content screening and may help overcome the limitations of existing ex vivo assessments of vascular contraction. This assay will aid in the reduction of animal use in labs studying vascular biology, a key tenant of the 3Rs principles (replacement, reduction, refinement)...” And just to conclude this discussion, from other of my related collaborations, we have the next one [20], which is quoted for the incursion into the highly delicate field of “Scanning Acoustic Microscopy (SAM)”, that has no need of staining [21]; then, [22] has been recently quoted for [23], in relation to the regulation of cellular calcification.…”