Microfluidics, BioMEMS, and Medical Microsystems XIX 2021
DOI: 10.1117/12.2578830
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Time-dependent pulsing of microfluidic pumps to enhance 3D bioprinting of peptide bioinks

Abstract: Using bioinks for 3D bioprinting of cellular constructs remains a challenge due to factors including viscosity, fluid dynamics and shear stress. The encapsulation of cells within the bioinks directly affects the quality of 3D bioprinting and microfluidic pumping is a commonly used supporting approach. The accuracy of microfluidic pumps can be further improved by introducing various mixing techniques. However, many of these techniques introduce complex geometries or external fields. In this study, we used a sim… Show more

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Cited by 5 publications
(9 citation statements)
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“…Another technique employed to better facilitate 3D bioprinting was automated pulsing using the 3D bioprinting system's microfluidic pumps. As reported in [17], this technique allows for smoother printing by regulating the flowrate at different time intervals. By observing the user's manual adjustments to the pump flow during printing, a square pulse wave profile was created to mimic the adjustments through automation.…”
Section: Discussionmentioning
confidence: 97%
See 3 more Smart Citations
“…Another technique employed to better facilitate 3D bioprinting was automated pulsing using the 3D bioprinting system's microfluidic pumps. As reported in [17], this technique allows for smoother printing by regulating the flowrate at different time intervals. By observing the user's manual adjustments to the pump flow during printing, a square pulse wave profile was created to mimic the adjustments through automation.…”
Section: Discussionmentioning
confidence: 97%
“…Another key factor in 3D bioprinting of the mouse brain model to scale was maintaining seamless flow of peptide bioink through the nozzle without excess gelation or inconsistencies. To achieve this, a protocol involving automated time-dependent pulsing was implemented with microfluidic pumps; this protocol had previously been optimized for printing lines and basic grid shapes [17]. Pumping with a square pulse flow profile was found to assist in maintaining continuous flow by alternately reducing peptide and PBS flow rates at specific time points while maintaining the overall volume in the nozzle at any given time constant at 80 µl min −1 .…”
Section: D Bioprinting Of a Vm Da And Vm Non-da Neuronal Modelmentioning
confidence: 99%
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“…To ensure a thorough sampling of bioprinting experiments for the dataset, the data was generated by multiple users and printing stations, using ultrashort peptides of varied batches, sequences, and concentrations, as well as different concentrations of PBS. Different flow profiles were also taken into account to incorporate manual and automated flow cases 24 . Each of these parameters were bounded by optimization ranges previously established for precise printing with our system 11,14,15 .…”
Section: Methodology 21 Data Collectionmentioning
confidence: 99%