Motion reconstruction for rigid bodies and rigid-body frames using data from inertial measurement units (IMUs) is a challenging task. Position and orientation determination by means of IMUs is erroneous, as deterministic and stochastic errors accumulate over time. The former of which errors can be minimized by standard calibration approaches, however, sensor calibration with respect to a common reference coordinate system to correct misalignment, has not been fully addressed yet. The latter stochastic errors are mostly reduced using sensor fusion. In this paper, we present a novel motion-reconstruction method utilizing optimization to correct measured IMU data by means of correction polynomials to minimize the deviation of motion constraints. In addition, we perform gyrometer and accelerometer calibration with an industrial manipulator to address deterministic IMU errors, especially misalignment. To evaluate the performance of the novel methods, two types of experiments, one at constant orientation and another with simultaneous translation and rotation, were conducted utilizing the manipulator. The experiments were repeated for five individual IMUs successively. Application of the calibration and optimization methods yielded an average decrease of 95% in the maximum position error compared to the results of common motion reconstruction. Moreover, the average position error over the measurement duration decreased by nearly 90%. The proposed method is applicable to velocity, position, and orientation constraints for every experiment that starts and ends at standstill.