Time-lapse videography of human embryos: Using pronuclear fading rather than insemination in IVF and ICSI cycles removes inconsistencies in time to reach early cleavage milestones

Liu, Yanhe; Chapple, Vincent; Feenan, Katie; Roberts, Peter; Matson, Phillip

doi:10.1016/j.repbio.2015.03.002

Cited by 30 publications

(19 citation statements)

References 15 publications

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“…Since this initial report, other studies have confirmed the importance of early cleavage divisions and identified additional imaging parameters predictive of developmental success (Cruz et al 2011; Meseguer et al 2011; Azzarello et al 2012; Cruz et al 2012; Dal Canto et al 2012; Hashimoto et al 2012; Hlinka et al 2012; Meseguer et al 2012; Rubio et al 2012; Desai et al 2014; Y. Liu et al 2014a, 2014b, 2015; Stensen et al 2015; Fig. 2).…”

Section: Non-invasive Methods For Aneuploidy Detectionmentioning

confidence: 86%

Chromosomal instability in mammalian pre-implantation embryos: potential causes, detection methods, and clinical consequences

Daughtry

Chavez

2015

Cell Tissue Res

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Formation of a totipotent blastocyst capable of implantation is one of the first major milestones in early mammalian embryogenesis, but less than half of in vitro fertilized embryos from most mammals will progress to this stage of development. Whole chromosomal abnormalities, or aneuploidy, are key determinants of whether human embryos will arrest or reach the blastocyst stage. Depending on the type of chromosomal abnormality, however, certain embryos still form blastocysts and may be morphologically indistinguishable from chromosomally normal embryos. Despite the implementation of pre-implantation genetic screening and other advanced in vitro fertilization (IVF) techniques, the identification of aneuploid embryos remains complicated by high rates of mosaicism, atypical cell division, cellular fragmentation, sub-chromosomal instability, and micro-/multi-nucleation. Moreover, several of these processes occur in vivo following natural human conception, suggesting that they are not simply a consequence of culture conditions. Recent technological achievements in genetic, epigenetic, chromosomal, and non-invasive imaging have provided additional embryo assessment approaches, particularly at the single-cell level, and clinical trials investigating their efficacy are continuing to emerge. In this review, we summarize the potential mechanisms by which aneuploidy may arise, the various detection methods, and the technical advances (such as time-lapse imaging, “-omic” profiling, and next-generation sequencing) that have assisted in obtaining this data. We also discuss the possibility of aneuploidy resolution in embryos via various corrective mechanisms, including multi-polar divisions, fragment resorption, endoreduplication, and blastomere exclusion, and conclude by examining the potential implications of these findings for IVF success and human fecundity.

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Section: Non-invasive Methods For Aneuploidy Detectionmentioning

confidence: 86%

Chromosomal instability in mammalian pre-implantation embryos: potential causes, detection methods, and clinical consequences

Daughtry

Chavez

2015

Cell Tissue Res

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“…and rechecked by a third person (D.B.). To control for different insemination techniques used (34% and 66% of the cohort was fertilized with conventional IVF or ICSI, respectively) static morphokinetic variables were standardized to the common postfertilization time point of pronuclear fading (26)(27)(28).…”

Section: Time-lapse Annotationsmentioning

confidence: 99%

Blastocyst collapse is not an independent predictor of reduced live birth: a time-lapse study

Bodri¹,

Sugimoto²,

Serna³

et al. 2016

Fertility and Sterility

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“…Annotations were done using EmbryoViewer by two experienced embryologists and re-checked by a third person. In order to control for the different insemination techniques used (38 and 62 % of the cohort was fertilized with conventional IVF or ICSI, respectively), static morphokinetic variables were standardized to a common time point of pronuclear fading [8,29,30].…”

Section: Time-lapse Annotationsmentioning

confidence: 99%

Time-lapse variables and embryo gender: a retrospective analysis of 81 live births obtained following minimal stimulation and single embryo transfer

Bodri¹,

Kawachiya²,

Sugimoto³

et al. 2016

J Assist Reprod Genet

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Time-lapse videography of human embryos: Using pronuclear fading rather than insemination in IVF and ICSI cycles removes inconsistencies in time to reach early cleavage milestones

Cited by 30 publications

References 15 publications

Chromosomal instability in mammalian pre-implantation embryos: potential causes, detection methods, and clinical consequences

Chromosomal instability in mammalian pre-implantation embryos: potential causes, detection methods, and clinical consequences

Blastocyst collapse is not an independent predictor of reduced live birth: a time-lapse study

Time-lapse variables and embryo gender: a retrospective analysis of 81 live births obtained following minimal stimulation and single embryo transfer

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