Time-lapse variables and embryo gender: a retrospective analysis of 81 live births obtained following minimal stimulation and single embryo transfer

Bodri, Daniel; Kawachiya, Satoshi; Sugimoto, T.; Serna, Jazmina Yao; Kato, Ryutaro; Matsumoto, Tsunekazu

doi:10.1007/s10815-016-0678-4

Cited by 14 publications

(9 citation statements)

References 34 publications

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“…Bronet and colleagues were able to build a hierarchical model based on s2 (t4-t3)<2 h and tM between 80.8–98.3 h, that increased the likelihood -though not significant- of selecting female embryos after cleavage stage biopsy on day 3 [ 64 ]. Another study explored TLM parameters and gender in untested blastocysts based on the gender upon live birth and concluded that female embryos are strongly associated with late expanded blastocyst TLM parameters [ 65 ]. However, as untested blastocysts were not only transferred in HRT cycles but also in natural cycles, it cannot be guaranteed that all live births were obtained from the respective transferred blastocyst or were obtained from spontaneous pregnancies.…”

Section: Discussionmentioning

confidence: 99%

Marginal differences in preimplantation morphokinetics between conventional IVF and ICSI in patients with preimplantation genetic testing for aneuploidy (PGT-A): A sibling oocyte study

et al. 2022

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Objective This study aimed to analyze the morphokinetic behaviour between conventional IVF and ICSI, in cycles with preimplantation genetic testing for aneuploidies (PGT-A). Materials A randomized controlled trial (NCT03708991) was conducted in a private fertility center. Thirty couples with non-male factor infertility were recruited between November 2018 and April 2019. A total of 568 sibling cumulus oocyte complexes were randomly inseminated with conventional IVF and ICSI and cultured in an Embryoscope time-lapse system. The morphokinetic behaviour of IVF/ICSI sibling oocytes was analysed as primary endpoint. As secondary endpoints, morphokinetic parameters that predict blastocysts that will be biopsied, the day of biopsy, gender and euploid outcome was assessed. Results When comparing IVF to ICSI, only the time to reach the 2-cell stage (t2) was significantly delayed for IVF embryos: OR: 1.282 [1.020–1.612], p = 0.033. After standardizing for tPNf (ct parameters), only Blast(tStartBlastulation-t2) remained significant: OR: 0.803 [0.648–0.994], p = 0.044. For the analysis of zygotes that will be biopsied on day 5/6 versus zygotes without biopsy, only early morphokinetic parameters were considered. All parameters were different in the multivariate model: ct2: OR: 0.840 [0.709–0.996], p = 0.045; ct6: OR: 0.943 [0.890–0.998], p = 0.043; cc2(t3-t2): OR: 1.148 [1.044–1.263], p = 0.004; cc3(t5-t3): OR: 1.177 [1.107–1.251], p<0.0001. When comparing the development between blastocysts biopsied on day 5 versus day 6, only three morphokinetic parameters were significant: cc2(t3-t2): OR: 1.394 [1.010–1.926], p = 0.044; ctBlastocyst: OR: 0.613 [0.489–0.768], p<0.0001 and ctExpandedBlastocyst: OR: 0.913 [0.868–0.960], p = 0.0004. Multivariate analysis of gender and ploidy did not reveal differences in morphokinetic behaviour. Conclusion Minor morphokinetic differences are observed between IVF and ICSI. Early in the development, distinct cleavage patterns are observed between embryos that will be biopsied or not.

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Section: Discussionmentioning

confidence: 99%

Marginal differences in preimplantation morphokinetics between conventional IVF and ICSI in patients with preimplantation genetic testing for aneuploidy (PGT-A): A sibling oocyte study

et al. 2022

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“…Sex-associated morphokinetics at later stages of embryonic development has also been a topic of ongoing debate. For example, a recent retrospective analysis showed an increase in time to reach the fully expanded blastocyst stage in male embryos [23], while other studies have found that the development of male embryos was faster than female embryos, as "t3 had a significant correlation with live birth sex" [24]. Meanwhile, Serdarogullari et al [25] found no statistical significance in the kinetic profiles between sexes, from early cleavage events through to the blastocyst stage.…”

Section: Discussionmentioning

confidence: 96%

Morphometric and morphokinetic differences in the sperm- and oocyte-originated pronuclei of male and female human zygotes: a time-lapse study

Orevich

Watson

Ong

et al. 2022

J Assist Reprod Genet

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Purpose To study the morphometric and morphokinetic profiles of pronuclei (PN) between male and female human zygotes. Method(s) This retrospective cohort study included 94 consecutive autologous single day 5 transfer cycles leading to a singleton live birth. All oocytes were placed in the EmbryoScope + incubator post-sperm injection with all annotations performed retrospectively by one embryologist (L-SO). Timing parameters included 2nd polar body extrusion (tPB2), sperm-originated PN (tSPNa) or oocyte-originated PN (tOPNa) appearance, and PN fading (tPNF). Morphometrics were evaluated at 8 (stage 1), 4 (stage 2), and 0 h before PNF (stage 3), measuring PN area (um 2 ), PN juxtaposition, and nucleolar precursor bodies (NPB) arrangement. Results Male zygotes had longer time intervals of tPB2_tSPNa than female zygotes (4.8 ± 0.2 vs 4.2 ± 0.1 h, OR = 1.442, 95% CI 1.009-2.061, p = 0.044). SPN increased in size from stage 1 through 2 to 3 (435.3 ± 7.2, 506.7 ± 8.0, and 556.3 ± 8.9 um 2 , p = 0.000) and OPN did similarly (399.0 ± 6.1, 464.3 ± 6.7, and 513.8 ± 6.5 um 2 , p = 0.000), with SPN being significantly larger than OPN at each stage (p < 0.05 respectively). More male than female zygotes reached central PN juxtaposition at stage 1 (76.7% vs 51.0%, p = 0.010), stage 2 (97.7% vs 86.3%, p = 0.048), and stage 3 (97.7% vs 86.3%, p = 0.048). More OPN showed aligned NPBs than in SPN at stage 1 only (44.7% vs 28.7%, p = 0.023). Conclusion(s)Embryos with different sexes display different morphokinetic and morphometric features at the zygotic stage.Embryo selection using such parameters may lead to unbalanced sex ratio in resulting offspring.

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“…Results indicated that female status is related to late cleavage (t8), morula (tM), and blastocyst-stage morphokinetic parameters. The authors concluded that some expanded blastocyst-stage morphokinetic variables are correlated with female embryo gender [25]. Otherwise, in another study 176 male and 161 female embryos were evaluated, and there were remarkable diferences between embryo developmental time points and intervals regarding gender.…”

Section: Efect Of Gender Status On Embryo Morphokineticsmentioning

confidence: 93%

Embryo Morphokinetics Based on Time-Lapse Observation

Tabibnejad¹

2017

Embryo Cleavage

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Embryo incubation and evaluation are critical steps in assisted reproductive technology (ART). Conventionally, embryo assessment has been done by embryologists through removing embryos from a conventional incubator during the culture period. Over recent years, time-lapse systems (TLS) have been established which can take digital images of embryos at key points and time intervals. This technique allows embryologists to assess the embryo quality in the steady culture environment. According to TLS studies and prepared algorithm models, it seems that TLS alone or in combination with conventional morphology can be considered as a useful diagnostic tool to determine high-quality embryos and improve embryonic implantation and pregnancy rates. In addition, there were remarkable diferences between embryo developmental time points and intervals regarding embryo gender, embryo fragmentation, and type of ovarian stimulation protocol. For conident conclusion, time-lapse imaging should be evaluated in further studies, and the system should be evaluated for cost/beneit ratio efectiveness in individual laboratory.Keywords: embryo cleavage, embryo morphokinetics, embryoscope, time-lapse imaging IntroductionAssisted reproductive technology (ART) may help infertile couples to realize their dream to have a child in their family, but pregnancy and live birth rates following in vitro fertilization (IVF) still remain low. It is ideal to identify viable embryos with the highest implantation potential to raise IVF success rates. In the traditional IVF practice, embryo assessments are mainly based on the morphologic observation and grade of embryologists at each stage of oocyte and embryonic development. Some features including oocyte and embryo quality, blastomere numbers and regularity, the percentage of fragmentation, and cytoplasmic granularity have been deined as prognostic indicators of successful pregnancy. This traditional embryo assessment method may have some detrimental efects on embryo growth because frequently opening and closing of incubators often cause to the change of embryo culture environmental steadiness. In order to reduce the inter-and intra-observer diference change, the time-lapse imaging (TLI) has been introduced into in vitro fertilization (IVF) laboratory. The application of time-lapse technology to the clinical IVF laboratory has supported more detailed observations on the embryo development researches quickly.The aim of this chapter is to determine whether TLI is useful for selection of "top quality" embryos for transfer to improve ART outcome rather than conventional morphological evaluation. The possible correlation between embryos' sex, embryo fragmentation, treatment protocols, diferent culture media, and embryo morphokinetics will be examined based on some new researches of TLI facilities. Furthermore, various algorithms and predictive models designed in ART cycles with TLI will be discussed.

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Time-lapse variables and embryo gender: a retrospective analysis of 81 live births obtained following minimal stimulation and single embryo transfer

Cited by 14 publications

References 34 publications

Marginal differences in preimplantation morphokinetics between conventional IVF and ICSI in patients with preimplantation genetic testing for aneuploidy (PGT-A): A sibling oocyte study

Marginal differences in preimplantation morphokinetics between conventional IVF and ICSI in patients with preimplantation genetic testing for aneuploidy (PGT-A): A sibling oocyte study

Morphometric and morphokinetic differences in the sperm- and oocyte-originated pronuclei of male and female human zygotes: a time-lapse study

Embryo Morphokinetics Based on Time-Lapse Observation

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