Time-of-flight magnetic flow cytometry in whole blood with integrated sample preparation

Helou, Michael; Reisbeck, Mathias; Tedde, Sandro F.; Richter, Lukas; Bär, L.; Bosch, Johanna L.; Stauber, Roland H.; Quandt, Eckhard; Hayden, Oliver

doi:10.1039/c3lc41310a

Cited by 60 publications

(51 citation statements)

References 14 publications

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“…Our results are directly applicable to confined modes in isolated spintronic 18 or magnonic 44 nanostructures. This is encouraging for the development of frequency-based spintronic devices such as spin torque oscillators for nano-scale magnetic biosensing 18 in applications such as flow cytometry 30,31 . Notably our observed frequency shifts are significantly larger than measured spin torque oscillator linewidths 27,45,46 however electrical detection will rely on close proximity of the MNP to the sensing layer and high GHz/T field sensitivities.…”

Section: Discussionmentioning

confidence: 99%

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Sensing magnetic nanoparticles using nano-confined ferromagnetic resonances in a magnonic crystal

Metaxas¹,

Sushruth²,

Begley³

et al. 2015

Applied Physics Letters

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We demonstrate the use of the magnetic-field-dependence of highly spatially confined, GHz-frequency ferromagnetic resonances in a ferromagnetic nanostructure for the detection of adsorbed magnetic nanoparticles. This is achieved in a large area magnonic crystal consisting of a thin ferromagnetic film containing a periodic array of closely spaced, nano-scale anti-dots. Stray fields from nanoparticles within the anti-dots modify resonant dynamic magnetisation modes in the surrounding magnonic crystal, generating easily measurable resonance peak shifts. The shifts are comparable to the resonance linewidths for high anti-dot filling fractions with their signs and magnitudes dependent upon the modes' localisations (in agreement with micromagnetic simulation results). This is a highly encouraging result for the development of frequencybased nanoparticle detectors for high speed nano-scale biosensing.Magnetic biosensors, in which biological analytes are tagged with magnetic nanoparticles (MNPs), have excellent potential for solid-state point-of-care medical diagnostics 1-3 . The technique is intrinsically matrix-insesntive 1 , can compete with industry-standard immunoassays 4 and can be combined with magnetic separation methods 5 . The central element of a magnetic biosensor is a detector for the stray or 'fringing' magnetic fields generated by magnetised MNPs which are used to label, typically in-vitro, analytes of interest within a biological sample. Previously used sensors include SQuIDs 6 , Hall sensors 7 , ferromagnetic rings 8,9 and magneto-impedance devices 10 . However one of the most widely used methods is that employing magnetoresistive (MR) magnetic field sensors [1][2][3][4][5][11][12][13][14] which are typically fabricated with at least one

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Section: Discussionmentioning

confidence: 99%

“…therein) which have been predicted to retain high field sensing signal to noise ratios at sub-100 nm dimensions 18,19 . Furthermore, real time electrical detection of the dynamics [25][26][27] will pave the way for high speed 19 , nanoscale MNP sensing for solid-state flow cytometry [28][29][30][31] .…”

mentioning

confidence: 99%

Sensing magnetic nanoparticles using nano-confined ferromagnetic resonances in a magnonic crystal

Metaxas¹,

Sushruth²,

Begley³

et al. 2015

Applied Physics Letters

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“…Also, extensive surface functionalization with biomolecules, such as antibodies tailored to achieve specific targeting of cell types and/or organs, or 'cellular uptake proteins' does not completely prevent corona formation, albeit such modifications clearly affect corona profiles. In some cases, protein corona formation was even considered a major factor significantly reduced cell-targeting efficacy in vitro as well as in vivo (97,(105)(106)(107).…”

Section: Prevention Strategies To Inhibit Corona Formationmentioning

confidence: 99%

The bio-corona and its impact on nanomaterial toxicity

Westmeier

Chen²,

Stauber

et al. 2015

European Journal of Nanomedicine

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Abstract:The rapidly growing application of nanosized materials and nano-scaled processes will result in increased exposure of humans and the environment. The small size of nanomaterials (NM) comparable with molecular building blocks of cells raises concerns that their toxic potential cannot be extrapolated from studies of larger particles due to their unique physico-chemical properties. These properties are also responsible that NM rapidly adsorb various (bio)molecules when introduced into complex physiological or natural environments. As the thus formed protein/biomolecule 'corona' seems to affect the NM' in situ identity, an understanding of its toxicological relevance and the biophysical forces regulating corona formation is needed but not yet achieved. This review introduces our current concept of corona formation and evolution and present analytical methods for corona profiling. We discuss toxicity mechanisms potentially affected by the biomolecule corona, including NM cellular uptake and impact on components of the blood system. Further, we comment on pending knowledge gaps and challenges, which need to be resolved by the field. We conclude by presenting a tiered systems biology-driven approach recommended to mechanistically understand the coronas' nanotoxicological relevance and predictive potential.

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“…As regards the cell detection, [9][10][11][12][13][14][15] GMR sensors have been used in the recent works to count the number of magnetically labeled cells at high speed. However, compared with detecting biomolecules marked by magnetic micro-or nanobeads, the research of detecting cells using magnetic devices are still less.…”

Section: Wavy Ferromagnetic Device As Single Cell Detectionmentioning

confidence: 99%

Wavy ferromagnetic device as single cell detection

Huang

Ger

Lin

et al. 2014

Journal of Applied Physics

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We demonstrate a design of using a wavy permalloy thin film as a cell sensing device for the purpose of single magnetic cell detection. The magnetoresistance curve (MR curve) differs according to the single magnetic cell attached to the surface. By analyzing the MR curves, we can determine the sensing capability of the permalloy magnetic film device. Our results indicate that the sensitivity of the permalloy film sensing devices with wavy surface is much higher than the devices with flat surface. When a single magnetic cell is captured by the wavy surface of the permalloy film, the switching field of the film increase which is caused by the stray field of the magnetic cell. We discover that the highest sensitivity occurs when the direction of the magnetic field is along the Z-axis, and there is significant potential for the application of cell detection.

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Time-of-flight magnetic flow cytometry in whole blood with integrated sample preparation

Cited by 60 publications

References 14 publications

Sensing magnetic nanoparticles using nano-confined ferromagnetic resonances in a magnonic crystal

Sensing magnetic nanoparticles using nano-confined ferromagnetic resonances in a magnonic crystal

The bio-corona and its impact on nanomaterial toxicity

Wavy ferromagnetic device as single cell detection

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