Time-resolved 3D confocal fluorescence microscopy on living cells

Schellenberg, M.; Peev, E.; Kloster, Michael; Napier, J.; Neu, W.

doi:10.1117/12.871459

Cited by 2 publications

(1 citation statement)

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“…Also, compressive sensing microscopy based on a DMD was investigated for optical sectioning of pollen grains [23]. Time-resolved 3D confocal fluorescence microscopy was developed using a DMD with limited maximum scan thickness [24].…”

Section: Introductionmentioning

confidence: 99%

In Situ Fluorescence Optical Detection Using a Digital Micromirror Device (DMD) for 3D Cell-based Assays

Choi¹,

Kim

Kim³

2012

Journal of the Optical Society of Korea

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We have developed a fluorescence optical detection system using a digital micromirror device (DMD) for monitoring 3D cell culture matrices in situ. Full 3D imaging with fast scanning speed was implemented by the combined action of a DMD and a motorized stage. Imaging results with fluorescent microbeads measure the minimum axial resolution of the system as 6.3 μm, while full 1-mm scanning through 3D alginate-based matrix was demonstrated. For cell imaging, improved images were obtained by removing background fluorescence although the scanning distance was reduced because of low intracellular fluorescence efficiency. The system is expected to be useful to study various dynamics and behaviors of 3-dimensionally cultured cells in microfluidic systems.

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Section: Introductionmentioning

confidence: 99%

In Situ Fluorescence Optical Detection Using a Digital Micromirror Device (DMD) for 3D Cell-based Assays

Choi¹,

Kim

Kim³

2012

Journal of the Optical Society of Korea

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Massively parallel confocal dark field microscopy

Napier

Schellenberg

Kauer

et al. 2023

Emerging Digital Micromirror Device Based Systems and Applications XV

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A new technique which combines the advantages of darkfield microscopy with those of confocal microscopy has been developed. The Massively Parallel Confocal darkfield method implemented on a DMD-based confocal platform allows for detection of non-fluorescing particles with dimensions below the diffraction limit. The lateral resolution and depth discrimination of three dimensional objects are improved relative to conventional darkfield microscopy.

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Time-resolved 3D confocal fluorescence microscopy on living cells

Cited by 2 publications

References 0 publications

In Situ Fluorescence Optical Detection Using a Digital Micromirror Device (DMD) for 3D Cell-based Assays

In Situ Fluorescence Optical Detection Using a Digital Micromirror Device (DMD) for 3D Cell-based Assays

Massively parallel confocal dark field microscopy

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