Time-resolved fluorescence aptamer-based sandwich assay for thrombin detection

An electrochemical aptasensor for thrombin detection based on the recycling of exonuclease III and double-stranded DNA-templated copper nanoparticles assisted signal amplification

“…The regression equation is y = 0.9018-0.1584x, where y is the peak current (mA); x is the logarithm of the concentration of thrombin (pM), r = 0.995. The detection limit is 20.3 fM defined as 3s (where s is the standard deviation of the zero standards), which is much lower than the previous methods by using time-resolved fluorescence aptamer-based sandwich assay (7.7 nM), SERS-based magnetic aptasensor (0.27 pM) or hemin/G-quadruplex-based pseudobienzyme electrochemical detection (0.15 pM) [36][37][38]. The relative standard deviation for three times of repetitive analysis of different concentrations of thrombin can all within 5%, and the average of the relative standard deviations is 2.21%, demonstrating that well reproducibility of the proposed method.…”

Spectroscopic and Electrochemical Detection of Thrombin/5'-SH or 3'-SH Aptamer Immobilized on (porous) Gold Substrates

“…11,12 An aptamer is an artificial oligonucleotide (DNA or RNA) that can bind various targets, such as metal ions, small molecules, proteins, and cells, with high selectivity and specificity. 13 Additionally, aptamers have advantages for immobilization, such as ease of labeling, good stability, simple preparation, and being inexpensive.…”

“…[8][9][10][11][12][13][14][15][16][17][18] Among these sensing technologies, electrochemical aptasensors have attracted particular attention because they provide a simple, sensitive, and selective platform for thrombin detection. A SPR spectroscopic aptasensor allows use of a microsecond time-resolved change of SPR reflectance.…”

Atomic force microscopy fishing of gp120 on immobilized aptamer and its mass spectrometry identification

“…Поэтому они широко используются в аналитических, диагностических и терапевтических приложениях [6]. Использование аптамеров в качестве аффинных реагентов было продемонстрировано в [7][8][9][10][11][12], где их взаимодействие с белками регистрировалось с помощью детекторов на основе флуоресцентного [7][8][9] и электрохимического анализов [10,11], а также поверхностного плазмонного резонанса [12]. Во всех этих подходах анализ проводился на основе измерения свойств ансамбля большого количества молекул.…”