Time-resolved Fluorescence Resonance Energy Transfer Assay for Point-of-Care Testing of Urinary Albumin

Qin, Qiuping; Peltola, O.; Pettersson, Kim

doi:10.1373/49.7.1105

Cited by 54 publications

(30 citation statements)

References 42 publications

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“…It also showed an increased risk of major cardiovascular events at increased levels of urinary albumin excretion including levels within the normal range. (18) The use of ACE inhibitor, Ramipril prevents or delays the progression of albuminuria (19) although the effect of ACE inhibitor was not included in the present study. In our study, it was seen that as age of the patient increased the percentage showing microalbuminuria increased significantly and correlated with earlier studies.…”

Section: Discussionmentioning

confidence: 99%

Microalbuminuria Predicts Renal and Cardiovascular Dysfunction in Patients With Type 2 Diabetes Mellitus

Osman¹,

Nair²

2017

jemds

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Section: Discussionmentioning

confidence: 99%

Microalbuminuria Predicts Renal and Cardiovascular Dysfunction in Patients With Type 2 Diabetes Mellitus

Osman¹,

Nair²

2017

jemds

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“…It is particularly well suited to high-throughput screening in the drug discovery industry (25,46) and in clinical settings (36). It has successfully been applied to the detection of analytes within human sera (3,19) and has been shown to work in either the competitive format (6) or the sandwich format (1).…”

Section: Discussionmentioning

confidence: 99%

Time-Resolved Fluorescent Resonance Energy Transfer Assay for Simple and Rapid Detection of Anti-BrucellaAntibodies in Ruminant Serum Samples

et al. 2009

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Brucellosis is a globally significant zoonosis, the control of which is difficult and resource intensive. Serological tests form a vital part of a multifactorial approach to control and are often performed in large numbers. The aim of the present study was to develop a new assay to improve the efficiency, ease, and effectiveness of serological testing. An existing competitive enzyme-linked immunosorbent assay (cELISA) was adapted to a completely homogeneous time-resolved fluorescent resonance energy transfer (TR-FRET) assay. This was achieved by labeling an anti-Brucella monoclonal antibody with a long-lifetime donor fluorophore and Brucella smooth lipopolysaccharide with a compatible acceptor and optimizing the reading conditions. The assay was performed in a 96-well plate with a single 30-min incubation period and no separation (wash) steps and was concluded by a single plate-reading step. The performance of the assay was evaluated with a panel of serum samples from infected (n ‫؍‬ 73) and uninfected (n ‫؍‬ 480) sources and compared to the performance of the parent cELISA, an indirect ELISA (iELISA), and fluorescence polarization assay (FPA). The performance of the TR-FRET assay matched the performance of the iELISA, which had 100% diagnostic sensitivity and specificity, and surpassed the performance of the cELISA and the FPA. The results also demonstrated that the TR-FRET technique is effective with poor-quality serum samples from the field. To the knowledge of the authors, this is the first homogeneous TR-FRET assay to detect antibodies raised against an infectious disease. The technique appears to be sufficiently adaptable to meet the needs of many other similar testing requirements to identify infectious diseases.Brucellosis is a zoonosis of widespread distribution and significance caused by species of the genus Brucella. The disease is known to be especially prevalent in the Middle East and Mediterranean Basin (33), and there are disturbing signs of its reemergence across large areas of the globe, especially in central Asia. Few countries have successfully managed to eradicate the disease. In many of these countries, although the livestock sector is disease free, a significant wildlife reservoir remains and presents a risk of reintroduction (11).The principle etiologic agents of brucellosis are the classical smooth species Brucella abortus, B. melitensis, and B. suis. These species all have smooth lipopolysaccharide (sLPS), which is a major virulence factor (17, 35). Each of these species has a preferred host; however, many animals, including humans, are susceptible to each of the classical species, although the underlying mechanisms for host preference are not clearly understood (5). Brucellosis in ruminants is mainly manifested by reproductive failure due to abortion. There are few other clinical signs, and this causes difficulties with diagnosis. In humans, the disease is mainly presented as an undulating febrile condition, although there may be other, more serious complications. The symptoms of human ...

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“…The difference plot (Fig. 6b) versus the known values showed that 96 of the 102 samples had a difference between −27.98 % and 26.14 %, and 78 of the 102 samples had a difference between −14.45 % and 12.61 %, indicating that the proposed method is accurate [17].…”

Section: Accuracymentioning

confidence: 92%

A sensitive chemiluminescent immunoassay for point-of-care testing of repaglinide in natural dietary supplements and serum

Zheng

Wang

et al. 2015

Anal Bioanal Chem

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For point-of-care testing of the illegal fortification of repaglinide (Rep) in natural dietary supplements, a competitive chemiluminescent immunoassay (CLIA) was established, using a horseradish peroxidase (HRP)-luminol-H2O2 system for signal amplification. Polyclonal antibodies for Rep were produced via immunization technique. Following optimization of the enzyme reaction time and concentrations of antibody and coating antigen, the method showed a limit of quantification (LOQ) of 1.0 ng/mL in PBS and limit of detection (LOD) of 8.3 ng/mL in serum and 6.0 ng/mL in blank tablets. When applied in natural dietary supplements, the method provided results consistent with those from HPLC, suggesting that the proposed method could be used for rapid screening of Rep in natural dietary supplements and detecting Rep in serum after administration.

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Time-resolved Fluorescence Resonance Energy Transfer Assay for Point-of-Care Testing of Urinary Albumin

Cited by 54 publications

References 42 publications

Microalbuminuria Predicts Renal and Cardiovascular Dysfunction in Patients With Type 2 Diabetes Mellitus

Microalbuminuria Predicts Renal and Cardiovascular Dysfunction in Patients With Type 2 Diabetes Mellitus

Time-Resolved Fluorescent Resonance Energy Transfer Assay for Simple and Rapid Detection of Anti-BrucellaAntibodies in Ruminant Serum Samples

A sensitive chemiluminescent immunoassay for point-of-care testing of repaglinide in natural dietary supplements and serum

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