Time Sequence of Airway Remodeling in a Mouse Model of Chronic Asthma: the Relation with Airway Hyperresponsiveness

Kim, Seung Joon; Kim, Chi Hong; Ahn, Joong Hyun; Kim, Myung Sook; Kim, Seok Chan; Lee, Sook Young; Kwon, Soon Seog; Kim, Young Kyoon; Kim, Kwan Hyoung; Moon, Hwa Sik; Song, Jeong Sup; Park, Sung Hak

doi:10.3346/jkms.2007.22.2.183

Cited by 14 publications

(6 citation statements)

References 29 publications

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“…2). Our results reinforce previous findings by showing that changes in lung mechanics in sensitised mice seem to be related to airway inflammation and remodelling [31][32][33].…”

Section: Discussionsupporting

confidence: 92%

“…Previous studies using animal models have shown that after 30 days exposure to OVA inhalation mice present with airway remodelling [30][31][32]. In the present study, the AT was started after 28 days of OVA inhalation and our results demonstrate that AT reduces OVA-induced airway remodelling, including collagen fibre deposition, smooth muscle thickness and mucus production in this murine model of asthma ( fig.…”

Section: Discussionsupporting

confidence: 73%

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Aerobic training reverses airway inflammation and remodelling in an asthma murine model

Silva¹,

Vieira²,

Duarte³

et al. 2009

Eur Respir J

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Aerobic training (AT) decreases dyspnoea and exercise-induced bronchospasm, and improves aerobic capacity and quality of life; however, the mechanisms for such benefits remain poorly understood. The aim of the present study was to evaluate the AT effects in a chronic model of allergic lung inflammation in mice after the establishment of airway inflammation and remodelling.Mice were divided into the control group, AT group, ovalbumin (OVA) group or OVA+AT group and exposed to saline or OVA. AT was started on day 28 for 60 min five times per week for 4 weeks. Respiratory mechanics, specific immunoglobulin (Ig)E and IgG 1 , collagen and elastic fibres deposition, smooth muscle thickness, epithelial mucus, and peribronchial density of eosinophils, CD3+ and CD4+, IL-4, IL-5, IL-13, interferon-c, IL-2, IL-1ra, IL-10, nuclear factor (NF)-kB and Foxp3 were evaluated.The OVA group showed an increase in IgE and IgG 1 , eosinophils, CD3+, CD4+, IL-4, IL-5, IL-13, NF-kB, collagen and elastic, mucus synthesis, smooth muscle thickness and lung tissue resistance and elastance. The OVA+AT group demonstrated an increase of IgE and IgG 1 , and reduction of eosinophils, CD3+, CD4+, IL-4, IL-5, IL-13, NF-kB, airway remodelling, mucus synthesis, smooth muscle thickness and tissue resistance and elastance compared with the OVA group (p,0.05). The OVA+AT group also showed an increase in IL-10 and IL-1ra (p,0.05), independently of Foxp3.AT reversed airway inflammation and remodelling and T-helper cell 2 response, and improved respiratory mechanics. These results seem to occur due to an increase in the expression of IL-10 and IL-1ra and a decrease of NF-kB.

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“…2). Our results reinforce previous findings by showing that changes in lung mechanics in sensitised mice seem to be related to airway inflammation and remodelling [31][32][33].…”

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 73%

Aerobic training reverses airway inflammation and remodelling in an asthma murine model

Silva¹,

Vieira²,

Duarte³

et al. 2009

Eur Respir J

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show abstract

“…In this study, we used a chronic OVA model where we found gradual reduction of airway inflammation and gradual increase in structural changes upon repeated OVA exposures. Similar findings were also observed by other groups [22]. We focused on the effect of MEP in this model with the administration of the drug after initiation of airway remodeling, and found that it efficiently attenuated airway remodeling.…”

Section: Discussionsupporting

confidence: 79%

Mepacrine treatment attenuates allergic airway remodeling segregated from airway inflammation in mice

Ahmad¹,

Mabalirajan²,

Hasija³

et al. 2011

International Immunopharmacology

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“…Light microscopy (x200 magnification) was used for histopathological assessment. For the semi-quantification of goblet cell hyperplasia, slides were examined in a double-blind setting using a semi-quantitative scoring system as previously described (16,17) with certain modifications. Briefly, pathological changes were evaluated according to the modified 5-point scoring system (grade 0-4) and were expressed by score according to the percentage of the goblet cells in the epithelium: 0 (no goblet cells), 1 (<25%), 2 (25-50%), 3 (51-75%) and 4 (>75%).…”

Section: Methodsmentioning

confidence: 99%

Atractylodin ameliorates ovalbumin‑induced asthma in a mouse model and exerts immunomodulatory effects on Th2 immunity and dendritic cell function

et al. 2020

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asthma is a leading allergic disease worldwide, demonstrating an ever-increasing prevalence over the past two decades. asthma is characterized by allergen-associated airway hyperresponsiveness (aHr) that primarily results from T helper 2 (Th2) cell inflammation, in which dendritic cells (dcs) serve an important role in determining T cell development after encountering an antigen. atractylodin (aTl), a polyethene alkyne extracted from Atractylodis rhizoma (also known as cangzhu), has proven effective in treating digestive disorders, rheumatic disease and influenza. In addition, ATL was discovered to alleviate mouse collagen-induced arthritis via regulating dc maturation. The present study aimed to investigate the effect of aTl on asthma given that dcs serve an essential role in Th2-mediated inflammation in asthma. Mouse model of asthma was induced by ovalbumin (oVa). OVA-induced airway hyperresponsiveness (AHR) and inflammatory cells in bronchoalveolar lavage fluid (BALF) were detected. The production of ige and igG1 in serum and cytokines in BALF were detected by ELISA. The effects of ATL on dendritic cells maturation and T cell expansion were detected by flow cytometry analysis and 3H-thymidine incorporation. using a model of oVa-induced asthma, it was demonstrated that aTl ameliorated aHr and decreased the levels of il-4, IL-5 and IL-13 in bronchoalveolar lavage fluid (BALF), and OVA-specific IgE and IgG1 in the serum. OVA-stimulated splenocytes were used to demonstrated that aTl decreased cell expansion and the production of IL-4, IL-5 and IL-13 in the culture medium. in order to determine the cellular mechanism of aTl in asthma, splenic dcs were isolated and it was subsequently observed that aTl downregulated the expression levels of CD40 and CD80. Furthermore, OVA-stimulated CD4 + T cells were co-cultured with splenic dcs, which revealed that aTl-treated splenic dcs led to impaired cellular proliferation and the production of IL-4, IL-5 and IL-13 in OVA-stimulated T cells. in conclusion, these results indicated that aTl may suppress antigen-specific Th2 responses in an OVA-induced allergic asthma model via regulating dcs. Therefore, aTl may exhibit therapeutic potential in the management of asthma and other allergic diseases presenting with Th2 inflammation.Yu-cHao lin 1-3 , cHinG-cHieH YanG 4-6 , cHinG-HSiunG lin 7 , Te-cHun HSia 1-3 , Wen-cHenG cHao 8-10 and cHi-cHien lin 11,12

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Time Sequence of Airway Remodeling in a Mouse Model of Chronic Asthma: the Relation with Airway Hyperresponsiveness

Cited by 14 publications

References 29 publications

Aerobic training reverses airway inflammation and remodelling in an asthma murine model

Aerobic training reverses airway inflammation and remodelling in an asthma murine model

Mepacrine treatment attenuates allergic airway remodeling segregated from airway inflammation in mice

Atractylodin ameliorates ovalbumin‑induced asthma in a mouse model and exerts immunomodulatory effects on Th2 immunity and dendritic cell function

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