Time to Positivity and Detection of Growth in Anaerobic Blood Culture Vials Predict the Presence of Candida glabrata in Candidemia: a Two-Center European Cohort Study

Cobos-Trigueros, Nazaret; Kaasch, Achim J.; Soriano, Álex; Torres, Jorge-Luis; Vergara, Andrea; Morata, Laura; Zboromyrska, Yuliya; Calle, C. de la; Alejo, Izaskun; Hernández, Cristina; Cardozo, Celia; Marco, Francesc; Rı́o, Ana del; Almela, Manel; Mensa, Josep; Martínez, José Antonio

doi:10.1128/jcm.01198-14

Cited by 21 publications

(21 citation statements)

References 13 publications

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“…We observed a significantly prolonged TTP in the fungemia with C. glabrata as described in the previous studies [9][10][11].…”

Section: Discussionsupporting

confidence: 88%

“…This succeeded to advocate its presence more frequently and even faster in the anaerobic bottles than in the aerobic ones. A previous study using BACTEC 9240 system demonstrated this feature which distinguished fungemia of C. glabrata with more than 90% of specificity and negative predictive value, but 33% of sensitivity using only the criteria of earlier growth in the anaerobic bottles [9]. In our study, it was not highly sensitive (38.8%) to presume C. glabrata using the same criteria, either.…”

Section: Discussioncontrasting

confidence: 63%

“…C. glabrata demonstrated a longer time to positivity (TTP) compared to non-C. glabrata both in the Bactec system (Becton-Dickinson, Sparks, MD, USA) and BacT/Alert 3D system (bioMereiux Inc., Durham, NC, USA) [7,[9][10][11][12]. Time to positivity (TTP) is the time lapse from the insertion of the blood culture bottles into the continuous monitoring blood culture systems to the positive signals of microorganisms.…”

Section: Introductionmentioning

confidence: 99%

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Usefulness of Two-Step Algorithm with Earlier Growth Detection in Anaerobic Bottle and Time to Positivity to Predict Candida glabrata Fungemia

2018

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“…We observed a significantly prolonged TTP in the fungemia with C. glabrata as described in the previous studies [9][10][11].…”

Section: Discussionsupporting

confidence: 88%

Section: Discussioncontrasting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

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Usefulness of Two-Step Algorithm with Earlier Growth Detection in Anaerobic Bottle and Time to Positivity to Predict Candida glabrata Fungemia

2018

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“…Individually, C. glabrata accounted for 34.5%, C. albicans accounted for 30.6%, and C. parapsilosis accounted for 8.9% of the clinical isolates. An interesting observation was that C. glabrata was the yeast species present in 85.4% (35/41) of the clinical anaerobic bottles studied, a finding in agreement with a recent publication documenting exclusive or earlier growth of C. glabrata in anaerobic vials in a large series of candidemic patients from two European hospitals (33).…”

Section: Resultssupporting

confidence: 77%

Multicenter Evaluation of Candida QuickFISH BC for Identification of Candida Species Directly from Blood Culture Bottles

et al. 2015

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A total of 415 samples contained a single fungal species, with C. glabrata (n ‫؍‬ 129; 30.8%) being the most common isolate, followed by C. albicans (n ‫؍‬ 125; 29.8%), C. parapsilosis (n ‫؍‬ 59; 14.1%), C. tropicalis (n ‫؍‬ 37; 8.8%), and C. krusei (n ‫؍‬ 17; 4.1%). The overall agreement (with range for the three major Candida species) between the two methods was 99.3% (98.3 to 100%), with a sensitivity of 99.7% (98.3 to 100%) and a specificity of 98.0% (99.4 to 100%). This study showed that Candida QuickFISH BC is a rapid and accurate method for identifying C. albicans, C. glabrata, and C. parapsilosis, the three most common Candida species causing BSI, directly from blood culture bottles. Candida species are ranked as the third or fourth most common etiologic agents associated with nosocomial bloodstream infections (1, 2). Mortality rates are estimated to be as high as 45% (3, 4), due in part to slow diagnostic methods and to inappropriate initial antifungal treatment (5, 6). Candida albicans and other yeasts reside as commensals of the skin and mucous membranes of the gastrointestinal and genitourinary tracts, causing infection when the host becomes debilitated or immunocompromised. Risk factors for invasive candidiasis include central venous catheters, parenteral nutrition, organ transplantation, hemodialysis, surgery, burns, long-term stay in an intensive care unit, and previous administration of broad spectrum antimicrobial and immunosuppressive agents (7,8).The most common Candida species found in healthy individuals include C. albicans, C. glabrata, C. tropicalis, and C. parapsilosis (9). These species cause more than 90% of invasive infections (10). However, the prevalence of these different Candida species varies widely geographically, and their antifungal susceptibilities are species specific (11). Consequently, rapid, accurate identification of the causative Candida species is critical for successful treatment.Currently, blood culture is the gold standard method for the diagnosis of fungal bloodstream infection (12). When growth is detected, blood culture medium is drawn for Gram stain and subculture on agar plates for subsequent phenotypic identification (13), which typically takes at least 72 h (14). To overcome this relatively long turnaround time of identification, several molecular methods have been evaluated for identification of yeasts directly from blood culture bottles such as real-time PCR, matrixassisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry, and peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) (15,16). A method to detect Candida directly from patient's blood specimens within 3 h has also been developed (17, 18).The first generation of yeast PNA-FISH (AdvanDx, Woburn, MA) distinguishes between the five commonest Candida species directly from positive blood cultures within 90 min. The method uses fluorescence-labeled probes to complement species-specific rRNA sequences: C. albicans and C. parapsilosis fluoresce green, C. tropicalis fluor...

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“…The prevalence of C. glabrata in bloodstream infections could be underestimated due to methodological reasons as some isolates of C. glabrata may grow in culture under anaerobic conditions only . In addition, if C. glabrata grows under aerobic conditions, it does so more slowly than other Candida species In an investigation done by Hui et al .…”

Section: Epidemiologymentioning

confidence: 99%

Candida glabrata – unique features and challenges in the clinical management of invasive infections

Glöckner

Cornely

2015

Mycoses

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Candida glabrata is a pathogenic yeast with several unique biological features. This article provides an up-to-date review on current data and reasoning aspects of this clinically problematic organism. Haploidy, absence of pseudohyphae, facultative anaerobe growth of C. glabrata, as well as its intrinsically low susceptibility to azole antifungals require specific consideration in diagnosis and treatment approaches. As C. glabrata today represents a sizeable percentage of pathogens in candidaemia, the use of azole antifungals in upfront therapy of invasive yeast infections is discouraged by recent guidelines. While the selection of C. glabrata mutants with impaired susceptibility to echinocandins has been described, analyses of several clinical studies indicate an association of improved outcomes with the use of echinocandins as the primary treatment for invasive yeast infections with potential or documented involvement of C. glabrata.

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Time to Positivity and Detection of Growth in Anaerobic Blood Culture Vials Predict the Presence of Candida glabrata in Candidemia: a Two-Center European Cohort Study

Cited by 21 publications

References 13 publications

Usefulness of Two-Step Algorithm with Earlier Growth Detection in Anaerobic Bottle and Time to Positivity to Predict Candida glabrata Fungemia

Usefulness of Two-Step Algorithm with Earlier Growth Detection in Anaerobic Bottle and Time to Positivity to Predict Candida glabrata Fungemia

Multicenter Evaluation of Candida QuickFISH BC for Identification of Candida Species Directly from Blood Culture Bottles

Candida glabrata – unique features and challenges in the clinical management of invasive infections

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