TINGKAT KETERJADIAN PENYAKIT LAYU STEWART PADA BENIH DAN RESPON BEBERAPA VARIETAS JAGUNG TERHADAP INFEKSI Pantoea stewartii subsp. stewartii

Abstract

Disease incidence of Stewart’s wilt on the seed and response of several maize varieties to Pantoea stewartii subp. stewartii. Stewart’s wilt disease of maize is caused by Pantoea stewartii subsp. stewartii. This bacterium is seed-borne pathogens, when attacked maize caused yield lost 40-100%. The objective of this research was to detemine the incidence level of stewart’s wilt disease, growth of some varieties of maize and their response to stewart’s wilt pathogens Pantoea stewartii subsp. stewartii. The research was conducted in the Laboratory of Bacteriology and Greenhouse Cikabayan IPB from November 2011 to March 2012. In experiment I, nineteen samples of maize were used for symptom test in the maize seedling stage, using Randomized Block Design with three replications. Experiment II used a Randomized Block Design with 2 factors: maize varieties (8 hybrids varieties, 3 open pollinated varieties, and 7 sweet corn varieties) and bacteria isolates (BGR 2, BGR 4, BGR 28, BGR7 and PSM 27), with three replications. The results showed in experiment I, the incidence of stewart’s wilt disease ranged 2.00 – 15.33%, germination and vigor index of maize seed were 68.00 – 95.33% and 55.33 – 90.67% respectively. While in experiment II, hybrid and open pollinated of maize varieties were resistant to moderately susceptible while all sweet corn varieties were susceptible to infection of Pantoea stewartii subsp. stewartii.

Screening of plant growth-promoting endophytic bacteria from the maize roots for biocontrol of stewart wilt disease

“…In PGSA, other species grow slowly because of their crystal violet and 65% NaCl content (figure 1). Based on the physiological test, Pantoea is categorized as Gram-negative, catalase-positive, oxidasenegative, and reacting positively to Hugh and Leifson's OF media [23]. The hypersensitivity test results that isolates were positive, as seen by the necrosis symptoms in tobacco leaves about 48-72 hours after inoculation.…”

“…Wet spots around the inoculation site appear after three days and are followed by lengthy chlorosis on the leaves after 5-7 days. The latent period of wilt disease ranges from 3 to 6 days after inoculation [23]. The host plant's reaction, aggressive pathogens, and environmental circumstances affect infection [23].…”

“…The latent period of wilt disease ranges from 3 to 6 days after inoculation [23]. The host plant's reaction, aggressive pathogens, and environmental circumstances affect infection [23].…”

The causal agent and the distribution of maize stalk rot disease in the Tanah Laut Regency, South Kalimantan

“…Two maize seeds were sown in sterilized soil prepared in a plastic cup. After seven days, the seedlings were selected and then used for the plant test [7].…”

Status and Recent Developments of Pantoea stewartii subsp. stewartii Causes the Wilt Disease in Maize in Indonesia: A Review

“…Still, it has also spread to several large continents such as South America, Africa, Europe and Asia (EPPO, 2022a). In Indonesia, Stewart wilt disease of corn is known to have spread in the archipelago such as East Java, West Sumatra, West Java and Bali (Suryani et al, 2012;Rahma et al, 2013Rahma et al, , 2014Temaja et al, 2017) and other large islands such as Sulawesi and Lombok-West Southeast Nusa (Ministry of Agriculture, 2020). The application of detection technology and knowledge is also important for technical implementation in the field to detect the entry and endemic of this disease.…”