This study aims to experimentally examine the effects of vehicle speed changes on the vertical resonance frequency of effective rolling radius variations for applying to TPMS (Tire Pressure Monitoring System). For this, the road tests have been conducted at constant vehicle speeds 30, 60, 90km/h for tire pressures 15, 20, 25, 30, 35psi. In these tests, the effective rolling radius changes have been measured. Test results show that the vehicle speed changes cause the resonance frequency to shift at same tire pressure. Therefore, this study shows that the changes in vertical resonance frequency resulting from vehicle speed changes may cause wrong tire pressure information to be taken by TPMS.