Tissue adhesive FK506–loaded polymeric nanoparticles for multi–layered nano–shielding of pancreatic islets to enhance xenograft survival in a diabetic mouse model

Organ preservation with targeted rapamycin nanoparticles: a pre-treatment strategy preventing chronic rejection in vivo

“…Yet others advocate for the use of nanoparticles decorated with immunotherapeutics as a delivery device for tissues such as allo-islets to allow for implantation under the shield of immunomodulating therapeutics 27. Pharmacotheapeutic delivery of immunosuppressants via micro and nanoparticles have been touted to represent the “next era” of transplantation, as increased doses of drug may be delivered directly to the area of need.…”

Nanotechnology's application in Type 1 diabetes

“…Bryant et al (2014) investigated the utilizing of poly (lactide-co-glycolide) (PLG) nanoparticles instead of donor ECDI-SP to convey donor antigen to induce complete MHC-mismatched mouse islet allograft tolerance. Pham et al (2018) used 3,4-dihydroxyphenethylamine (DOPA) conjugated PLGA-PEG nanoparticles (DOPA-NPs) loaded with the immunosuppressant FK506 to form a generic antigenic camouflage coating layer without interfering with islet activity and function to enhance xenograft survival.…”

Optimization of nano-encapsulation on neonatal porcine islet-like cell clusters using polymersomes

“…To perform the glucose-stimulated insulin secretion (GSIS) assay, 20-30 NPCCs were picked and pre-incubated with a low D-glucose (2.8 mM) concentration in Krebs-Ringer bicarbonate buffer (KRBB) for 1 hr. NPCCs were then incubated with low D-glucose (2.8 mM) in KRBB buffer for 1 h followed by high D-glucose solution (28.0 mM) in KRBB for 1 h. Supernatants were collected to measure insulin secretion under low and high glucose concentrations [11]. The amount of insulin secreted from each sample was measured using a Human/Canine/Porcine Insulin Quantikine ELISA Kit (R&D systems, MN, USA).…”

“…In previous studies, a basic Hank's balanced salt solution (HBSS buffer, pH 8.0) was used as the NPCCs nano-encapsulation reaction buffer because of its ability to facilitate NHS-NH2 binding [10,11,12].…”