Tissue Alloantigens Present in Human Leukocytes*

Dausset, J; Iványi, P

doi:10.1111/j.1749-6632.1966.tb12866.x

Cited by 13 publications

(2 citation statements)

References 11 publications

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“…Specificity of the antisera was compared stati\tically by the method described by Dausset et al (1966). The number of similar and dissimilar reactions in every possible pair of functional antisera were counted and a correlation coefficient r was calculated.…”

Section: Statistical Evaluation Of the Antiseramentioning

confidence: 99%

Alloantigens Defined by Cytotoxic Isoantisera Prepared against Peripheral Rabbit Lymphocytes

Luster

Vice

1976

Tissue Antigens

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Seven lymphocytotoxic antisera were produced in rabbits by means of isoimmunization with purified peripheral lymphocytes. The phenotypic profile of the defined lymphocyte antigens was determined in a panel of 97 rabbits. The results indicated the existence of at least two major antigenic determinants. Further typing and cross‐absorptions were performed with several of the lymphocytotoxic antisera in order to demonstrate monospecificity. The anti‐3 antiserum was used to determine the distribution of the respective antigens in various rabbit organs. The highest concentration of the alloantigen was contained in the spleen, followed in descending order by the lymph nodes, lung, skin, kidney, heart and liver. Fat and red blood cells (rbc) contained no detectable antigen. A two‐stage physical adherence column method was used to separate T‐derived from B‐derived lymphocytes and each fraction was tested for the alloantigen. Alloantigen was detected on both cell types. The fact that the anti‐3 antiserum never killed more than 30% of the cells suggests that it could define a subpopulation of lymphocytes.

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Section: Statistical Evaluation Of the Antiseramentioning

confidence: 99%

Alloantigens Defined by Cytotoxic Isoantisera Prepared against Peripheral Rabbit Lymphocytes

Luster

Vice

1976

Tissue Antigens

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“…The reaction pattern of a series of antisera against a panel of cells has been used to establish similarity of antisera and to determine antisera that detect alleles in humans (Dausset et al 1966, Van Rood & Van Leeuwen 1963, and in the rabbit (Black 1967). In Table 111 the .4ntiserum 4997 anti- 499s Table II.…”

Section: Correlation Of Cytotoxic Antisrramentioning

confidence: 99%

Histocompatibility in the Rabbi Identification of the Major Locus¹²

Tissot

Cohen

1972

Tissue Antigens

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Cytotoxic antisera were produced either by skin graft exchange between related rabbits from the same partially inbred line or by graft exchange between rabbits from different inbreeding lines. By pedigree analysis and correlation with known antisera, these antisera were all shown to detect antigens controlled by a single locus. This locus has been assigned the symbol RL‐A. Skin grafts between siblings incompatible for the RL‐A were all rejected within twelve days, and graft survival time could not be prolonged with relatively mild immunosuppressive treatment with 6‐mercaptopurine. However, a significant number of grafts compatible for the RL‐A locus were maintained for twelve days or longer and graft survival time of compatible grafts could be significantly prolonged with mild immunosuppression, suggesting that the RL‐A locus is the major histocompatibility locus of the rabbit.

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