We determined 27 histocompatibility antigens of A, B, and C locus with a standard lymphocyte cytotoxicity test in 125 patients suffering from primary affective disorders, 77 of bipolar type, 24 of unipolar type, and 24 with schizo-affective psychosis. Comparison with a normal control group showed significant increases in the frequencies of antigens Bw40 and Cw4 in unipolar patients and a significantly decreased frequency of antigen Cw3 in bipolar patients. The statistical significances which were at the 5% level, disappeared when the P values were corrected for the number of antigens investigated. Our results failed to confirm previous findings of significantly altered antigen frequencies among patients with primary affective disorders.
Granulocytes were isolated b overlaying leukocyte-rich plasma discontinuous density gradients. The cell suspensions were 95 percent granulocytes. Sera of 383 polytransfused patients and 521 multiparous women were examined for lymphocytotoxins, granulocytotoxins, and granulocytoagglutinins. On the basis of these tests, sera were identified which contained only granulocytotoxins. These were further identified with more precision as to their titers and antibody absorption; reactivity with granulocytes, B, and T lymphocytes; and K 562 cell line. On the basis of calculated correlation coefficients, nine sera were selected which determined three granulocyte specificities having genotype frequencies consistent with the Hardy-Weinberg equilibrium.
SUMMARY
The effect of antibiotics on lymphocyte HL‐A antigens in vitro is of variable character. All antibiotics under examination suppressed the absorption capacity of HL‐A antigens after 2 hr of lymphocyte treatment at 37°C. Ceporin and Kanamytrex inhibited even the cytotoxic reactivity of H1‐A antigens after 15–30 min of lymphocyte treatment. Chloramphenicol, aureomycin, streptomycin and oleandomycin, on the contrary, increased the specific cytotoxic reactivity of HL‐A antigens after 15–30 min, after 1 hr they were ineffective for HL‐A antigens, and after two or more hours they produced polyreactivity. Penicillin and erythromycin produced polyreactivity after only 15–30 min. The results show that for the follow‐up of the drug effect on HL‐A antigens the absorption test rather than the cytotoxicity test is of importance. The suppressed absorption capacity of HL‐A antigens caused by the action of antibiotics proves their inactivation effect on the lymphocytes. The possibility of an analogous effect of antibiotics on lymphocyte HL‐A antigens, even after administration to patients, is discussed.
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