2017
DOI: 10.1016/j.actbio.2017.05.051
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Tissue and cellular biomechanics during corneal wound injury and repair

Abstract: Corneal wound healing is an enormously complex process that requires the simultaneous cellular integration of multiple soluble biochemical cues, as well as cellular responses to the intrinsic chemistry and biophysical attributes associated with the matrix of the wound space. Here, we document how the biomechanics of the corneal stroma are altered through the course of wound repair following keratoablative procedures in rabbits. Further we documented the influence that substrate stiffness has on stromal cell me… Show more

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Cited by 76 publications
(82 citation statements)
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“…While previous studies have focused on changes in the height of the undulations, our studies suggest that changes in the slope may also impact on the disruption of epidermal homeostasis that occurs in psoriatic lesions. In the case of corneal injury biomechanical changes in stromal cells are linked to accumulation of inflammatory cells [32] and it is therefore tempting to speculate that differences in the stiffness of epidermal cells and their junctions could lead to an altered immune infiltrate. It is also possible that materials that mimic the undulating topography of the epidermal-dermal junction, for example by casting topographies out of collagen gels, could enhance wound healing or reduce scarring.…”
Section: Discussionmentioning
confidence: 99%
“…While previous studies have focused on changes in the height of the undulations, our studies suggest that changes in the slope may also impact on the disruption of epidermal homeostasis that occurs in psoriatic lesions. In the case of corneal injury biomechanical changes in stromal cells are linked to accumulation of inflammatory cells [32] and it is therefore tempting to speculate that differences in the stiffness of epidermal cells and their junctions could lead to an altered immune infiltrate. It is also possible that materials that mimic the undulating topography of the epidermal-dermal junction, for example by casting topographies out of collagen gels, could enhance wound healing or reduce scarring.…”
Section: Discussionmentioning
confidence: 99%
“…The physiological conditions differ greatly from the in vitro settings, and at least up to this date, it is hard to reproduce the physiological conditions. Issues such as substrate stiffness will affect the in vitro experiments, and isolation of the cells from their natural environment will, to a degree, change them too . We expected to see a significantly bigger decrease in α‐SMA expression, as contraction of the collagen gels, in which the corneal fibroblasts are embedded, was decreased greatly by the ACh‐treated cells.…”
Section: Discussionmentioning
confidence: 99%
“…These keep depositing multiple elements of extracellular matrix to increase the corneal thickness [ 32 ]. By remodeling the healing stroma, and replacing the disorganized repair matrix with regular corneal extracellular matrix, a better transparency can be achieved [ 33 , 34 ]. In addition, the number and the diameter of new vessels slowly decreases as the remodeling progresses [ 35 ].…”
Section: Discussionmentioning
confidence: 99%