Tissue- and isoform-specific phytochrome regulation of light-dependent anthocyanin accumulation in
            <i>Arabidopsis thaliana</i>

Warnasooriya, Sankalpi N.; Porter, Katie; Montgomery, Beronda L.

doi:10.4161/psb.6.5.15084

Cited by 24 publications

(21 citation statements)

References 67 publications

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“…Our work here, as well as prior studies (Costigan et al 2011; Hopkins and Kiss 2012; Montgomery 2009; Warnasooriya and Montgomery 2009; Oh and Montgomery 2011; Warnasooriya et al 2011), demonstrated that the use of distinct promoters or enhancer-trap-based tools for localized BVR expression to induce phytochrome chromophore degradation results in lines with distinct disruptions in phytochrome-dependent regulation of gene expression and phytochrome-deficient phenotypes. We identified a number of genes whose expression was differentially impacted by localized phytochrome deficiencies using microarray analyses.…”

Section: Discussionsupporting

confidence: 81%

“…Furthermore, the use of distinct promoters or enhancer-trap-based tools for localized BVR expression is useful for the generation of lines with distinct patterns of tissue-specific phytochrome deficiencies and associated discrete phytochrome-deficient phenotypes (Costigan et al 2011; Hopkins and Kiss 2012; Montgomery 2009; Warnasooriya and Montgomery 2009; Warnasooriya et al 2011). Notably, comparative analyses of CAB3::pBVR and 35S::pBVR3 lines indicated that CAB3::pBVR seedlings, which have mesophyll-specific phytochrome depletion, exhibit elongated hypocotyls relative to No-0 WT and constitutive 35S::pBVR3 seedlings under increasing FR fluences (Warnasooriya and Montgomery 2009).…”

Section: Introductionmentioning

confidence: 99%

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Downstream effectors of light- and phytochrome-dependent regulation of hypocotyl elongation in Arabidopsis thaliana

Warnasooriya

Montgomery

2013

Plant Mol Biol

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Arabidopsis, like most plants, exhibits tissue-specific, light-dependent growth responses. Cotyledon and leaf growth and the accumulation of photosynthetic pigments are promoted by light, whereas hypocotyl growth is inhibited. The identification and characterization of distinct phytochrome-dependent molecular effectors that are associated with these divergent tissue-specific, light-dependent growth responses are limited. To identify phytochrome-dependent factors that impact the photoregulation of hypocotyl length, we conducted comparative gene expression studies using Arabidopsis lines exhibiting distinct patterns of phytochrome chromophore inactivation and associated disparate hypocotyl elongation responses under far-red (FR) light. A large number of genes was misregulated in plants lacking mesophyll-specific phytochromes relative to constitutively-deficient phytochrome lines. We identified and characterized genes whose expression is impacted by light and by phyA and phyB that have roles in the photoregulation of hypocotyl length. We characterized the functions of several identified target genes by phenotyping of T-DNA mutants. Among these genes is a previously uncharacterized LHE (LIGHT-INDUCED HYPOCOTYL ELONGATION) gene, which we show impacts light- and phytochrome-mediated regulation of hypocotyl elongation under red (R) and FR illumination. We describe a new approach for identifying genes involved in light- and phytochrome-dependent, tissue-specific growth regulation and confirmed the roles of three such genes in the phytochrome-dependent photoregulation of hypocotyl length.Electronic supplementary materialThe online version of this article (doi:10.1007/s11103-013-0029-0) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Downstream effectors of light- and phytochrome-dependent regulation of hypocotyl elongation in Arabidopsis thaliana

Warnasooriya

Montgomery

2013

Plant Mol Biol

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“…Similar phenotypes were previously observed on replete medium at ambient temperatures. 13,41 The role of mesophyll-localized phyA, phyB and light-stable phytochromes in addition to phyB are shown here to be important additionally for regulating the induction of anthocyanins by low temperature and nutrient limitation ( Figs. 1-2; Table 1).…”

Section: Discussionmentioning

confidence: 84%

Mesophyll-localized phytochromes gate stress- and light-inducible anthocyanin accumulation inArabidopsis thaliana

Warnasooriya

Montgomery

2014

Plant Signaling & Behavior

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“…Tissue-specific induction of localized phytochrome deficiency has been used to provide molecular evidence for a role of cotyledon- or leaf-localized phytochromes in the regulation of hypocotyl elongation ( Montgomery, 2009 ; Warnasooriya and Montgomery, 2009 ), which had previously been observed at the physiological level using localized irradiation of plants (discussed above in Section “Microbeam Irradiation”). Additionally, the regulation of organ-specific anthocyanin accumulation was identified through targeted phytochrome ablation ( Warnasooriya et al, 2011 ). Such studies also led to insights into the roles of root-specific phytochromes and phytochrome-driven shoot–root interactions in the light-dependent regulation of root development and elongation ( Costigan et al, 2011 ; Warnasooriya and Montgomery, 2011a ; Hopkins and Kiss, 2012 ).…”

Section: Integrating Molecular Approaches Into Understanding Spatiotementioning

confidence: 99%

Spatiotemporal Phytochrome Signaling during Photomorphogenesis: From Physiology to Molecular Mechanisms and Back

Montgomery

2016

Front. Plant Sci.

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Light exposure results in distinct responses in specific seedling tissues during photomorphogenesis. Light promotes growth of cotyledons and leaves, as well as development and elongation of roots, whereas light inhibits elongation of hypocotyls. For distinct plant responses such as shade avoidance, far-red light or shifts in spectral light quality similarly have disparate impacts on distinct plant tissues, resulting in elongation of stems or petioles and a reduction in growth of leaf blades for many species. The physiological bases of such tissue- and organ-specific light responses were initially studied using localized irradiation of specific tissues and organs, or irradiation of dissected plant parts. These historical approaches were used to identify spatial-specific pools of photoreceptors responsible for regulating local, i.e., tissue- or organ-specific, or distal, i.e., interorgan, plant responses. The red/far-red responsive phytochromes have been the most widely studied among photoreceptors in this regard. Whereas, the spatial localization of photoreceptors regulating many tissue- or organ-specific light responses were identified, the underlying signaling networks responsible for mediating the observed responses have not been well defined. Recent approaches used to investigate the molecular bases of spatiotemporal light responses include selective irradiation of plants harboring mutations in specific photoreceptors, tissue-specific expression of photoreceptors, primarily in photoreceptor mutant backgrounds, or tissue-specific biochemical ablation of photoreceptor accumulation. Progressive integration of such approaches for regulating the availability of localized pools of phytochromes with the use of transcriptomic or proteomic analyses for assessing the genes or proteins which these spatially discrete pools of phytochrome regulate is yielding emergent insight into the molecular bases of spatiotemporal phytochrome signaling pathways responsible for regulating spatiotemporal light responses of which we have been aware of at the physiological level for decades. Here, I discuss historical and emerging approaches to elucidating spatiotemporal signaling mediated by phytochromes during photomorphogenesis.

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Tissue- and isoform-specific phytochrome regulation of light-dependent anthocyanin accumulation in Arabidopsis thaliana

Cited by 24 publications

References 67 publications

Downstream effectors of light- and phytochrome-dependent regulation of hypocotyl elongation in Arabidopsis thaliana

Downstream effectors of light- and phytochrome-dependent regulation of hypocotyl elongation in Arabidopsis thaliana

Mesophyll-localized phytochromes gate stress- and light-inducible anthocyanin accumulation inArabidopsis thaliana

Spatiotemporal Phytochrome Signaling during Photomorphogenesis: From Physiology to Molecular Mechanisms and Back

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