Tissue and stage-specific juvenile hormone esterase (JHE) and epoxide hydrolase (JHEH) enzyme activities and Jhe transcript abundance in lines of the cricket Gryllus assimilis artificially selected for plasma JHE activity: Implications for JHE microevolution

Characterization of two juvenile hormone epoxide hydrolases by RNA interference in the Colorado potato beetle

“…In B. mori, for example, JHEH was highly expressed in the gut, Malpighian tubule and silk gland (Cheng et al, 2014). In Gryllus assimilis, midgut and fat bodies JHEH activities exhibited a mid-stadium peak (Anand et al, 2008). Thus, our results supported the hypothesis proposed by Yang et al (2011), that different JHEHs, probably JHEs as well, mediate hydrolysis of JH in various tissues.…”

Juvenile hormone (JH) esterase activity but not JH epoxide hydrolase activity is downregulated in larval Adoxophyes honmai following nucleopolyhedroviruses infection

“…Thus, hemolymph JHE activity shows a strong and positive correlation with fat body JHE activity (Anand et al, 2008). As expected a sharp increase of JHE activity was found immediately prior to the prepupal stage (L5D2-L5D4) in mock-infected larval A. honmai.…”

“…2A). It has been demonstrated that JHEH activity in fat body has no correlation with both JHE activities in hemolymph and fat body (Anand et al, 2008). From these data, JHEH is not likely to have a role in the induction of pupation in A. honmai.…”

“…Total RNA from whole body was extracted every 24 h after inoculation as described in Section 2.5. b-actin of A. honmai was selected as a reference gene for qRT-PCR as suggested previously (Anand et al, 2008;Gatehouse et al, 2009). Primers were designed from ahjhe and partial sequence of b-actin of A. honmai.…”

“…JHEs have a signal peptide and are secreted enzymes and the activity is largely found in the hemolymph, whereas JHEH activity is membrane bound and found in the tissues, such as fat body and midgut (Anand et al, 2008;Kamita and Hammock, 2010). JHEH activities were not detectable in the hemolymph of Heliothis virescens (Lepidoptera: Noctuidae) or Bombyx mori (Lepidoptera: Bombycidae) (Li et al, 2003;Zhang et al, 2005).…”