Tissue bioconcentration and effects of fluoxetine in zebrafish (Danio rerio) and red crucian cap (Carassius auratus) after short-term and long-term exposure

Pan, Chenyuan; Yang, Ming; Xu, Hai; Xu, Bentuo; Jiang, Lihui; Wu, Minghong

doi:10.1016/j.chemosphere.2018.04.082

Cited by 47 publications

(21 citation statements)

References 30 publications

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“…Basic pharmaceuticals such as the selected serotonin reuptake inhibitors fluoxetine and sertraline, secondary amines with a pK a > 9.5 like the ionizable alkylamines studied here, accumulate to greater extents in the liver than in the muscle of fish exposed to sewage treatment plant effluents, 41,42 but not in fish subjected to controlled exposure. 43 Also amitriptyline, a phospholipophilic tertiary amine-based drug (pK a 9.8, log D MW 3.9) 44 showed higher accumulation in fish liver than in muscle (gilt-head bream) in a controlled bioconcentration study, 45 but the liver/plasma concentration ratios were not as high as the D L−B observed here. Higher values of D L−B compared to D Mus−B have also been observed for chloroquine, a basic pharmaceutical, and attributed to greater lysosomal sequestration in liver cells, 38 a process that has also been demonstrated for basic psychotropic drugs in slices of various rat tissues.…”

Section: ■ Resultsmentioning

confidence: 49%

Tissue Distribution of Several Series of Cationic Surfactants in Rainbow Trout (Oncorhynchus mykiss) Following Exposure via Water

Kierkegaard

Chen

Armitage

et al. 2020

Environ. Sci. Technol.

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Bioaccumulation assessment is important for cationic surfactants in light of their use in a wide variety of consumer products and industrial processes. Because they sorb strongly to natural surfaces and to cell membranes, their bioaccumulation behavior is expected to differ from other classes of chemicals. Divided over two mixtures, we exposed rainbow trout to water containing 10 alkyl amines and 2 quaternary alkylammonium surfactants for 7 days, analyzed different fish tissues for surfactant residues, and calculated the tissues' contribution to fish body burden. Mucus, skin, gills, liver, and muscle each contributed at least 10% of body burden for the majority of the test chemicals. This indicates that both sorption to external surfaces and systemic uptake contribute to bioaccumulation. In contrast to the analogue alkylamine bases, the permanently charged quaternary ammonium compounds accumulated mostly in the gills and were nearly absent in internal tissues, indicating that systemic uptake of the charged form of cationic surfactants is very slow. Muscle−blood distribution coefficients were close to 1 for all alkyl amines, whereas liver−blood distribution coefficients ranged from 13 to 90, suggesting that the dominant considerations for sorption in liver are different from those in blood and muscle. The significant fraction of body burden on external surfaces can have consequences for bioaccumulation assessment.

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Section: ■ Resultsmentioning

confidence: 49%

Tissue Distribution of Several Series of Cationic Surfactants in Rainbow Trout (Oncorhynchus mykiss) Following Exposure via Water

Kierkegaard

Chen

Armitage

et al. 2020

Environ. Sci. Technol.

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“…Human excretion of up to 10% of FLX parent compound and conjugated FLX glucuronide via the urine [1] and/or improper disposal have been reported to result in untreated urban WWTP influent concentrations of FLX as high as 3 μg/L [11]. In exposed fish, bioconcentration occurs and can reach factors >100, especially in slightly alkaline water conditions [13,15,35]. Tissue concentrations of FLX and its active metabolite NFLX are highest in brain and liver of wild-caught fish, reaching levels as high as 10 ng/g for FLX and 20 ng/g for NFLX [11,12,14].…”

Section: Introductionmentioning

confidence: 99%

Developmental fluoxetine exposure in zebrafish reduces offspring basal cortisol concentration via life stage-dependent maternal transmission

et al. 2019

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Fluoxetine (FLX) is a pharmaceutical used to treat affective disorders in humans, but as environmental contaminant also affects inadvertently exposed fish in urban watersheds. In humans and fish, acute FLX treatment and exposure are linked to endocrine disruption, including effects on the reproductive and stress axes. Using the zebrafish model, we build on the recent finding that developmental FLX exposure reduced cortisol production across generations, to determine possible parental and/or life-stage-dependent (age and/or breeding experience) contributions to this phenotype. Specifically, we combined control and developmentally FLX-exposed animals of both sexes (F 0 ) into four distinct breeding groups mated at 5 and 9 months, and measured offspring (F 1 ) basal cortisol at 12 dpf. Basal cortisol was lower in F 1 descended from developmentally FLX-exposed F 0 females bred at 5, but not 9 months, revealing a maternal, life-stage dependent effect. To investigate potential molecular contributions to this phenotype, we profiled maternally deposited transcripts involved in endocrine stress axis development and regulation, epigenetic ( de novo DNA methyltransferases) and post-transcriptional (miRNA pathway components and specific miRNAs) regulation of gene expression in unfertilized eggs. Maternal FLX exposure resulted in decreased transcript abundance of glucocorticoid receptor, dnmt3 paralogues and miRNA pathway components in eggs collected at 5 months, and increased transcript abundance of miRNA pathway components at 9 months. Specific miRNAs predicted to target stress axis transcripts decreased ( miR-740 ) or increased ( miR-26 , miR-30d , miR-92a , miR-103 ) in eggs collected from FLX females at 5 months. Increased abundance of miRNA-30d and miRNA-92a persisted in eggs collected from FLX females at 9 months. Clustering and principal component analyses of egg transcript profiles separated eggs collected from FLX-females at 5 months from other groups, suggesting that oocyte molecular signatures, and miRNAs in particular, may serve as predictive tools for the offspring phenotype of reduced basal cortisol in response to maternal FLX exposure.

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“…Pseudorasbora parva juveniles exposed for 42 days to 200 µg/L fluoxetine also displayed GST inhibition in the liver and gills [44]. The reduction in the activity of biotransformation enzymes could be related to the negative regulation of genes involved in the detoxification pathways, as observed by Cunha et al (2016) [40]. The effects of fluoxetine on antioxidant responses and biotransformation processes have been widely reported, with increased or decreased activity of enzymes belonging to these groups (CAT, SOD, GSH/GSSH, LPO/MDA, GST, and EROD activity).…”

Section: Discussionmentioning

confidence: 90%

“…The effects of fluoxetine on antioxidant responses and biotransformation processes have been widely reported, with increased or decreased activity of enzymes belonging to these groups (CAT, SOD, GSH/GSSH, LPO/MDA, GST, and EROD activity). The different fluoxetine concentrations tested, exposure lengths, species, and life stages tested may be related to the variability of responses [40][41][42][43][44]46]. However, all these studies evidence and support the potential of fluoxetine to promote long-term biochemical effects.…”

Section: Discussionmentioning

confidence: 96%

“…In the freshwater environment, concentrations of fluoxetine have been detected at levels ranging from 0.0004 to 3.645 µg/L in wastewater treatment plants (WWTP) [20][21][22][23][24][25][26][27][28][29][30][31], 0.0005 to 0.056 µg/L in surface waters and groundwaters [29,[31][32][33][34][35][36][37] and, for drinking water, the levels vary between 0.0005 and 0.0008 µg/L [38,39]. Previous studies have demonstrated that fluoxetine can be toxic to fish, with exposure resulting in changes at different biological levels, from gene transcription, neurotransmission markers, enzymatic activities (e.g., oxidative stress, metabolism), hormone levels, reproductive processes, and accumulation in various tissues (e.g., brain and liver), resulting in a severe change in the histology of these organs [4][5][6][7]10,11,14,[40][41][42][43][44][45][46][47][48]. In addition, this pharmaceutical can cause changes in behavior (e.g., locomotor activity, stress response, feeding, aggression, social and anti-predatory behavior) [4][5][6][7]…”

Section: Introductionmentioning

confidence: 99%

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Chronic Effects of Fluoxetine on Danio rerio: A Biochemical and Behavioral Perspective

et al. 2022

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Fluoxetine is an antidepressant widely used to treat depressive and anxiety states. Due to its mode of action in the central nervous system (selective serotonin reuptake inhibitor (SSRI)), it becomes toxic to non-target organisms, leading to changes that are harmful to their survival. In this work, the effects of fluoxetine on juvenile zebrafish (Danio rerio) were evaluated, assessing biochemical (phase II biotransformation—glutathione S-transferase (GST), neurotransmission—acetylcholinesterase (ChE), energy metabolism—lactate dehydrogenase (LDH), and oxidative stress—glutathione peroxidase (GPx)) and behavior endpoints (swimming behavior, social behavior, and thigmotaxis) after 21 days exposure to 0 (control), 0.1, 1 and 10 µg/L. Biochemically, although chronic exposure did not induce significant effects on neurotransmission and energy metabolism, GPx activity was decreased after exposure to 10 µg/L of fluoxetine. At a behavioral level, exploratory and social behavior was not affected. However, changes in the swimming pattern of exposed fish were observed in light and dark periods (decreased locomotor activity). Overall, the data show that juvenile fish chronically exposed to fluoxetine may exhibit behavioral changes, affecting their ability to respond to environmental stressors and the interaction with other fish.

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Tissue bioconcentration and effects of fluoxetine in zebrafish (Danio rerio) and red crucian cap (Carassius auratus) after short-term and long-term exposure

Cited by 47 publications

References 30 publications

Tissue Distribution of Several Series of Cationic Surfactants in Rainbow Trout (Oncorhynchus mykiss) Following Exposure via Water

Tissue Distribution of Several Series of Cationic Surfactants in Rainbow Trout (Oncorhynchus mykiss) Following Exposure via Water

Developmental fluoxetine exposure in zebrafish reduces offspring basal cortisol concentration via life stage-dependent maternal transmission

Chronic Effects of Fluoxetine on Danio rerio: A Biochemical and Behavioral Perspective

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