Tissue culture independent transformation of the forage crop sunnhemp (Crotalaria juncea L.): an easy method towards generation of transgenics

Rao, Jyothsna; Agrawal, Pushpa; Mahmood, Riaz; Sreevathsa, Rohini; Rao, K. Sankara; Reddy, G. R.; Suryanarayana, V. V. S.

doi:10.1007/s12298-011-0095-1

Cited by 5 publications

(2 citation statements)

References 30 publications

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“…Transgenic sunnhemp plants. Agrobacterium-mediated in planta transformation method for development of transgenic sunnhemp plants was described elsewhere (Rao et al, 2012). The germinating seeds of sunnhemp (Crotalaria juncea L. C.V. Bellary) were infected with vir gene-induced (Cheng et al, 1996) Agrobacterium host LBA4404 harboring the recombinant plasmid, pCAMBIA VP1 O-A.…”

Section: Methodsmentioning

confidence: 99%

Expression of VP1 protein of serotype A and O of foot-and-mouth disease virus in transgenic sunnhemp plants and its immunogenicity for guinea pigs

Rao¹,

Agrawal²,

Mohammad³

et al. 2012

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Recently, transgenic plants expressing immunogenic proteins of foot-and-mouth disease virus (FMDV) have been used as oral or parenteral vaccines against foot-and-mouth disease (FMD). They exhibit advantages like cost effectiveness, absence of processing, thermostability, and easy oral application. FMDV VP1 protein of single serotype has been mostly used as immunogen. Here we report the development of a bivalent vaccine with tandem-linked VP1 proteins of two serotypes, A and O, present in transgenic forage crop Crotalaria juncea. The expression of the bivalent protein in the transgenic plants was confirmed by Western blot analysis. Guinea pig reacted to orally or parenterally applied vaccine by humoral as well as cell-mediated immune responses including serum antibodies and stimulated lymphocytes, respectively. The vaccine protected the animals against a challenge with the virus of serotype A as well as O. This is the first report on the development of a bivalent FMD vaccine using a forage crop.

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Section: Methodsmentioning

confidence: 99%

Expression of VP1 protein of serotype A and O of foot-and-mouth disease virus in transgenic sunnhemp plants and its immunogenicity for guinea pigs

Rao¹,

Agrawal²,

Mohammad³

et al. 2012

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“…Machines for direct seeding and harvesting and herbicides for weed control are the main examples. (Larsen and Noack 2021;Rao and Madhulety 2005).…”

Section: Introductionmentioning

confidence: 99%

Yield and Growth Duration of Maroon rice landraces measured in traditional settings

Pinas,

Awie,

Dongstra

et al. 2024

Preprint

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Rice, the most consumed cereal worldwide, has two domesticated species: Asian and African rice. Commercial cultivars, almost all Asian rice, are mostly selected for high yields. Traditional landraces are often selected for different traits, such as time to maturity or resistance against common stress factors, including bird attacks and poor soils. Maroons, descendants of enslaved Africans in Suriname and French Guiana, cultivate a rich diversity of rice landraces. They categorize landraces into short, medium, and long-maturation varieties and maintain that fast-ripening crops with moderate yields facilitated their flight from enslavement, while once settled in a safe place, there was time for landraces with longer ripening periods and higher yields. Previous authors assumed that all Maroon landraces had low yields (700–1000 kg/ha), but their performance in traditional farming systems has not been studied. We followed the growth and harvest of 28 Maroon rice landraces and two commercial cultivars in Maroon farmer fields, using traditional farming methods, at three locations. We show that, in farmer-managed fields, Maroon rice can yield 2600 kg/ha (average 1665 kg/ha), without any agrochemicals or machinery. Contrastingly, the commercial cultivars on the Maroon farmer fields had a low yield (625–1205 kg/ha), partly due to bird predation. The maturation time varied between 110 to 183 days, but the three Maroon maturation categories showed significant overlap. Our study indicates that Maroon rice performance can only be fairly evaluated if measurements are taken in traditional Maroon farming systems since this is the environment in which the varieties were originally selected.

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In planta transformation: A smart way of crop improvement

Saifi

Passricha

Tuteja

et al. 2020

Advancement in Crop Improvement Techniques

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Tissue culture independent transformation of the forage crop sunnhemp (Crotalaria juncea L.): an easy method towards generation of transgenics

Cited by 5 publications

References 30 publications

Expression of VP1 protein of serotype A and O of foot-and-mouth disease virus in transgenic sunnhemp plants and its immunogenicity for guinea pigs

Expression of VP1 protein of serotype A and O of foot-and-mouth disease virus in transgenic sunnhemp plants and its immunogenicity for guinea pigs

Yield and Growth Duration of Maroon rice landraces measured in traditional settings

In planta transformation: A smart way of crop improvement

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