Tissue culture of Cyclamen spp.

Jalali, Neda; Naderi, Roohangiz; Shahi-Gharahlar, Ali; Silva, Jaime A. Teixeira da

doi:10.1016/j.scienta.2012.01.015

Cited by 33 publications

(17 citation statements)

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“…The genus Cyclamen is traditionally classified into the Primulaceae family, but in recent years it has been reclassified in the Myrsinaceae family and cultivated throughout temperate zones (Jalali et al, 2012). Containing only 22 species (Grey-Wilson, 2003), or 21 species according to Compton et al (2004) and Yesson and Culham (2006), the genus is relatively small, but is an attractive one.…”

Section: Introductionmentioning

confidence: 99%

Morphological characterization of Cyclamen sp. grown naturally in Turkey: Part I

Çürük

Söğüt

Bozdoğan

et al. 2015

South African Journal of Botany

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Twenty cyclamen taxa belonging to the Primulaceae originate from the Mediterranean region and grow under trees and bushes. There are 10 cyclamen species growing naturally in Turkey, five of which are endemic. In this study, intact cyclamen plants were collected from nature with their tubers intact. Collection took place in spring and autumn in Adana, Osmaniye, Kahramanmaraş, İzmir and Eskişehir provinces, 50 samples per location. Sampled plants were propagated and cultivated in Adana. The morphology of four Cyclamen species (C. persicum Mill., C. cilicium Boiss.e.Heldr., C. pseudibericum Hildebr. and C. coum Mill.) was characterized using 1-year-old regenerated plants. A total of 27 phenotypic characters (13 flower, 11 leaf, 2 plant, 1 tuber) were evaluated based on a detailed descriptor's list. In addition to these morphological observations, 13 quantitative traits (7 flower, 5 leaf, 1 tuber) were measured. The measurements related to morphological characteristics had a wide range of variation, including in tuber diameter, leaf length and width, petal length, petal color, and leaf shape, indicating the vast morphological differences among these four cyclamen species. In most cases, the use of principal component analysis confirmed the grouping of characters into species-specific clusters although one or two clusters could not differentiate species, indicating that morphological and cluster analyses alone are not enough for characterizing this complex Cyclamen germplasm and that molecular techniques may reveal more intricate and useful relationships.

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Section: Introductionmentioning

confidence: 99%

Morphological characterization of Cyclamen sp. grown naturally in Turkey: Part I

Çürük

Söğüt

Bozdoğan

et al. 2015

South African Journal of Botany

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“…(Figures 7 and 8). Moreover, the standardization of cost-effective and suitable mass multiplication strategies (both micro and macro propagation) [61,62] are important for reducing the cost of the plating materials and also for the large-scale popularization of such neglected and underutilized species because such mass multiplication strategies will make the cultivation of novel crops profitable to farmers. The creation of a national or regional inventory of crop wild relatives is another promising strategy [63,64].…”

Section: Popularizing the Unpopular: Final Stepmentioning

confidence: 99%

Domesticating the Undomesticated for Global Food and Nutritional Security: Four Steps

et al. 2019

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Ensuring the food and nutritional demand of the ever-growing human population is a major sustainability challenge for humanity in this Anthropocene. The cultivation of climate resilient, adaptive and underutilized wild crops along with modern crop varieties is proposed as an innovative strategy for managing future agricultural production under the changing environmental conditions. Such underutilized and neglected wild crops have been recently projected by the Food and Agricultural Organization of the United Nations as ‘future smart crops’ as they are not only hardy, and resilient to changing climatic conditions, but also rich in nutrients. They need only minimal care and input, and therefore, they can be easily grown in degraded and nutrient-poor soil also. Moreover, they can be used for improving the adaptive traits of modern crops. The contribution of such neglected, and underutilized crops and their wild relatives to global food production is estimated to be around 115–120 billion US$ per annum. Therefore, the exploitation of such lesser utilized and yet to be used wild crops is highly significant for climate resilient agriculture and thereby providing a good quality of life to one and all. Here we provide four steps, namely: (i) exploring the unexplored, (ii) refining the unrefined traits, (iii) cultivating the uncultivated, and (iv) popularizing the unpopular for the sustainable utilization of such wild crops as a resilient strategy for ensuring food and nutritional security and also urge the timely adoption of suitable frameworks for the large-scale exploitation of such wild species for achieving the UN Sustainable Development Goals.

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“…Callus formation was promoted by combining 1-2 mg l -1 2,4-D with a low cytokinin concentration (0.1-0.5 mg l -1 Kin) but suppressed by combining a higher concentration of auxin (2-4 mg l -1 2,4-D) and cytokinin ([0.5 mg l -1 Kin) (Jalali et al 2010b. The variation in callus formation might be explained by the degree of cellular sensitivity towards exogenously-applied PGRs which might be more important than their actual concentration due to the levels of endogenous PGRs (such as IAA) and activity of natural auxin and cytokinin oxidases in the tissue ( Van and Trinh 1990;Yamaner and Erdag 2008;Jalali et al 2012). During dedifferentiation, 2,4-D at relatively high concentrations acts simultaneously as an inducer and as an inhibitor of cell division so the process can be suppressed and cells enter a dormant stage (Fehér et al 2001;Jalali et al 2012).…”

Section: Differentiation Of Elss (Cim With Pgrs)mentioning

confidence: 99%

“…Organogenesis from somatic tissues has been widely described, mainly for C. persicum Mill. (Schwenkel and Grunewaldt 1988;Al-Majathoub 1999;Al-Majathoub and Karam 2000;Karam and Al-Majathoub 2000a, b;Abu-Qaoud 2004;Jalali et al 2012;Nhut et al 2012), but also for C. mirabile (Yamaner and Erdag 2008). Somatic embryogenesis has been found to be an efficient system for the in vitro propagation of C. persicum ; Motoyasu and Takiko 1991;Kreuger et al 1995;Schwenkel and Winkelmann 1998;Ruffoni et al 1998;Hohe et al 1999a, b;Winkelmann et al 2003;Winkelmann and Serek 2005;Winkelmann et al 2006;Naderi et al 2012;Kocak et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Development of an efficient regeneration protocol for four Cyclamen species endemic to Turkey

İzgü

Sevindik

Çürük

et al. 2016

Plant Cell Tiss Organ Cult

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In this study, embryo-like structures (ELSs) were induced in four endemic Turkish Cyclamen species (C. cilicium Boiss. et Heldr., C. parviflorum Pobed., C. mirabile Hildebr. and C. pseudibericum Hildebr.) in the presence of 13 combinations of two plant growth regulators (PGRs) (2,4-dichlorophenoxyacetic acid and 6-(c,cdimethylallylamino)purine) and four explant types (ovules, ovaries, leaves and petioles). The ratio of callus induction, different stages of ELS formation and the conversion of ELSs to plantlets were quantified. The most effective explant types for callus induction were leaves (56 % for C. cilicium and 59 % for C. parviflorum) and petioles (80 % for C. mirabile and 100 % for C. pseudibericum). Callus growth from the leaves and petioles of C. cilicium was 30 days earlier than that of C. mirabile and C. pseudibericum. In contrast, most callus formed from the petioles of C. pseudibericum (100 %) in medium with 2.5 mg l -1 2,4-D and 1 mg l -1 2iP. The highest number of ELSs was obtained succesfully from petioles (2.5 mg l -1 2,4-D and 1 mg l -1 2iP) and ovaries (2.5 mg l -1 2,4-D and 0.5 mg l -1 2iP) of C. pseudibericum, in 39 % and as 32 % of explants, respectively. The percentage conversion of ELSs to plantlets was 38, 31, 16 and 15 % for C. mirabile, C. cilicium, C. pseudibericum and C. parviflorum, respectively. The plantlets were successfully acclimatized in the greenhouse with 54, 70, 63 and 25 % of C. cilicium, C. mirabile, C. pseudibericum and C. parviflorum plantlets, respectively surviving after transfer to ex vitro conditions. This paper describes a unique, reliable and consistent protocol for the induction of ELSs from four endangered endemic Turkish Cyclamen species, opening up the possibility of preserving these valuable genetic resources in vitro and also other applied biotechnologies that rely on a stable embryogenic or callus-based protocol.

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Tissue culture of Cyclamen spp.

Cited by 33 publications

References 50 publications

Morphological characterization of Cyclamen sp. grown naturally in Turkey: Part I

Morphological characterization of Cyclamen sp. grown naturally in Turkey: Part I

Domesticating the Undomesticated for Global Food and Nutritional Security: Four Steps

Development of an efficient regeneration protocol for four Cyclamen species endemic to Turkey

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