Tissue culture of Lavandula angustifolia L.

Li, Nan-Yu; Tang, Haoru; Cong, Gao; Mo, Fan; Xiao, Yi-Hu; Luo, Yunbo

doi:10.1063/1.5092392

Cited by 4 publications

(10 citation statements)

References 2 publications

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“…In several studies, the proliferation of L. angustifolia explants was successfully performed in MS medium supplemented with other PGRs such as benzyladenine (BA) [60,61], 6-benzylaminopurine (BAP), thidiazuron (TDZ), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin, gibberellic acid (GA 3 ) [62][63][64][65][66][67], or zeatin [68]. In most studies, the large increase of the PGR concentration had a negative effect on shoot multiplication.…”

Section: Discussionmentioning

confidence: 99%

Effect of Jasmonic Acid and Salicylic Acid on Growth and Biochemical Composition of In-Vitro-Propagated Lavandula angustifolia Mill

et al. 2020

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This study assessed the effect of jasmonic acid (JA) and salicylic acid (SA) on the in vitro development and production of Lavandula angustifolia Mill. plant material, and the accumulation of polyphenols, chlorophylls, and carotenoids in explants. Results were compared with explants grown in control media and with in-vivo-grown mature and young L. angustifolia plants. After 21 days of incubation, all explants propagated on low-SA-concentration or elicitor-free media produced a greater number of shoots than explants cultivated on media with higher elicitor concentrations. Shoots grew taller when activated charcoal (AC) was added to the elicitor-supplemented media, while AC negatively affected or had no effect on the phytochemical composition of plants. Explants grown in the presence of elicitors had higher polyphenolic and chlorophyll content than the controls, demonstrating the beneficial impact of elicitors on the secretion of secondary metabolites. Lutein and β-carotene were the dominating carotenoids in all samples. Culture media supplemented with 0.5 mg/L JA and 1.5 mg/L SA + AC proved the most suitable to produce plant material with high polyphenol and carotenoid content, comparable with in-vivo-grown plants.

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Section: Discussionmentioning

confidence: 99%

Effect of Jasmonic Acid and Salicylic Acid on Growth and Biochemical Composition of In-Vitro-Propagated Lavandula angustifolia Mill

et al. 2020

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“…We chose fDNA because they have been shown to selectively bind a diverse range of non-nucleic acids targets, including organic small molecules, metal ions, proteins, viruses, and even cells and are obtained through a process called in vitro selection from a large DNA library of up to 10 15 sequences. [25][26][27][28] By coupling fDNA with CRISPR-Cas12a, we demonstrated that such a system can detect and quantify small organic molecules using aptamer-activated CRISPR-Cas12a and metal ions using DNAzyme-activated CRISPR-Cas12a under conditions required for POC devices, such as ambient temperatures and a simple and fast detection workflow.…”

Section: Introductionmentioning

confidence: 99%

Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets

et al. 2019

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Beyond its extraordinary genome editing ability, the CRISPR-Cas system has opened a new era of biosensing applications due to its high base resolution and isothermal signal amplification. However, the reported CRISPR-Cas sensors are largely only used for the detection of nucleic acids with limited application for non-nucleic acid targets. To realize the full potential of the CRISPR-Cas sensors and broaden their applications for detection and quantitation of non-nucleic acid targets, we herein report CRISPR-Cas12a sensors that are regulated by functional DNA (fDNA) molecules such as aptamers and DNAzymes that are selective for small organic molecule and metal ion detections. The sensor is based on the Cas12a dependent reporter system consisting of Cas12a, CRISPR RNA (crRNA) and its single stranded DNA substrate labeled with a fluorophore and quencher at each end (ssDNA-FQ), and fDNA molecules that can lock a DNA activator for Cas12a-crRNA, preventing the ssDNA cleavage function of Cas12a in the absence of the fDNA targets. The presence of fDNA targets can trigger the unlocking of the DNA activator, which can then activate the cleavage of ssDNA-FQ by Cas12a, resulting in an increase of the fluorescent signal detectable by commercially available portable fluorimeters. Using this method, ATP and Na + have been detected quantitatively under ambient temperature (25 °C) using a simple and fast detection workflow (two steps and <15 min), making the fDNA-regulated CRISPR system suitable for field tests or point-of-care diagnostics. Since fDNAs can be obtained to recognize a wide range of targets, the methods demonstrated here can expand this powerful CRISPR-Cas sensor system *

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“…The tissue culture technique is implemented to solve the production problem for lavender propagation. Various plant materials, including seed, seedlings, and explants, can be utilized to complete the procedure in this plant (Li et al, 2019). Each of the four steps of lavender micropropagationgermination, multiplication, rooting, and acclimatization requires a distinct in vitro media composition.…”

Section: Introductionmentioning

confidence: 99%

“…During the rooting process, plant hormones are not required. To help the plants get used to their new environment, they are moved to a greenhouse with the right substrate (Li et al, 2019;Koefender et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Silver nanoparticles eliminate hyperhydricity in micropropagated Lavender

Amnuaykan,

Nathewet,

Thepsukhon

2023

Ornam. Hortic.

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It is challenging to plant lavender outside of suitable conditions. This economically important plant requires optimal conditions to germinate and produce a high yield. To increase the quality of this plant, tissue culture is utilized. The most problematic aspect of lavender micropropagation is that cytokinin (CK) causes hyperhydricity (HH) during the shooting process. Consequently, this study was conducted to resolve HH in micropropagated lavender plantlets. Different concentrations of silver nanoparticles (AgNPs) were applied in conjunction with 1.0 mg L-1 6-benzylaminopurine (BA). Then, the performance of HH, growth and development, as well as total phenolic (TPC) and total flavonoid (TFC) content, were evaluated. The application of 20 mg L-1 of AgNPs was found to be the optimal method for halting HH. Although shoot proliferation was lower than in BA-supplemented media-grown plants, adding this concentration of AgNPs improved shoot and root quality. An increase in secondary metabolites and antioxidant activity may have caused the remedy of HH.

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Tissue culture of Lavandula angustifolia L.

Cited by 4 publications

References 2 publications

Effect of Jasmonic Acid and Salicylic Acid on Growth and Biochemical Composition of In-Vitro-Propagated Lavandula angustifolia Mill

Effect of Jasmonic Acid and Salicylic Acid on Growth and Biochemical Composition of In-Vitro-Propagated Lavandula angustifolia Mill

Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets

Silver nanoparticles eliminate hyperhydricity in micropropagated Lavender

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