Tissue culture : studies in experimental morphology and general physiology of tissue cells in vitro

Development a New Human Skin Continuous Cell Line Sensitive to Mumps Virus

“…This need comes from different degree of sensitivity that a virus shows to different cell substrate. Although tissue culture was started at the beginning of the twentieth century (13,14) as a technique for studying the behavior of animal cells, but at present is the most important tool in production of antigen (15) biological product, vaccine (16), isolation and detection of viruses (17,18). The primary explants of tissue dominated the field of 'tissue culture'' for more than 50 years (19,20).…”

Basic Techniques and Limitations in Establishing Cell Culture: a Mini Review

“…The culture of cells adopting explant method dominated the field of tissue culture for more than 50 years (Fisher, 1925;Parker, 1961). This is one of the simplest procedures, which involve finely chopping the tissue (explants of tissue) into fragments of no larger than 1-2 mm 3 .…”

“…This facilitated the subculture of adherent cells to produce homogeneous cell strains and marked the start of animal cell culture in true sense (Moscona, 1952). Till to that period, culture of cells from primary explants of tissue dominated the field (Fisher, 1925). The developments that occurred in over ten years (1940)(1941)(1942)(1943)(1944)(1945)(1946)(1947)(1948)(1949)(1950) made cell culture widely available and accepted as a tool for scientists.…”

Tissue Culture Methods in the Study of the Nervous System: A Review

“…To allow the tissue to float free in liquid was found to lead to prompt cessation of growth and steady degeneration of the cells (Uhlenhuth, 1915;Loeb, 1921;Fischer, 1925). If cells are to grow in a fluid medium then either there must be very little fluid so that capillary attraction will hold the tissue firmly on to the coverslip, or a mass of cottonwool may be incorporated so that the tissue is held firmly in its meshes.…”

“…Fischer (1925) has obtained in pure form the neuroepithelium of the chick retina, but it grew as a squamous epithelium instead of nervetissue. A further difficulty with nerve-tissue is, of course, that at an early age the spongioblasts differentiate from the neuroblasts, so that practically all nerve-tissue is ' contaminated' with either neuroglia or mesoblastic cells, or both.…”