Tissue Culture Variability in Wheat: Callus Induction and Plant Regeneration<sup>1</sup>

Sears, R. G.; Deckard, Edward L.

doi:10.2135/cropsci1982.0011183x002200030027x

Cited by 228 publications

(87 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In both varieties, 0.2 mg l Ϫ1 (0.9 mM) of TDZ ) on wheat regeneration of the two varieties were compared to those of other plant growth regulators and combinations commonly used for wheat regeneration. The plant growth regulators and combinations used in the experiments included 0.01 mg l Ϫ1 2,4-D (Sears and Deckerd, 1982;He et al, 1988), 0.5 mg l Ϫ1 dicamba (Hunsinger and Schauz, 1987;Weeks et al, 1993) and 1 mg l Ϫ1 kinetin + 1 mg l Ϫ1 NAA (Ouyang et al, 1983). In both varieties, TDZ resulted in the highest mean percentage regeneration (87% for Bob White and 49.4% for Hi-Line).…”

Section: Resultsmentioning

confidence: 99%

“…In wheat, TDZ (0.2 mg l Ϫ1 , 0.9 mM) was compared with 0.01 mg l Ϫ1 (0.045 mM) 2,4-D (Sears and Deckerd, 1982;He et al, 1988) and Schauz, 1987;Weeks et al, 1993), and a combination of 1 mg l Ϫ1 (4.6 mM) kinetin ϩ 1 mg l Ϫ1 (5.4 mM) a-naphthaleneacetic acid (NAA) (Ouyang et al, 1983). Most chemicals used in the experiments were purchased from Sigma Co. (St. Louis, MO) while TDZ was from Crescent Chemical Co. (Hauppauge, NY).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Thidiazuron promotes in vitro regeneration of wheat and barley

Shan

2000

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

SummaryThidiazuron (TDZ) is a substituted phenylurea which has been shown to be an efficacious regulator of in vitro morphogenesis of many dicot plant species. However, information regarding the effect of TDZ on in vitro regeneration of monocot species is limited. We investigated the effects of TDZ on in vitro regeneration of barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) and found that it promoted shoot regeneration from callus in these two important cereal species. Plant regeneration from calluses derived from immature embryo culture of barley and wheat was observed in regeneration media with a wide range of TDZ concentrations (0.045-45 mM). Shoot regeneration from barley calluses was the highest (38.3% for cv. Golden Promise) at 4.5 mM (1 mg l Ϫ1) TDZ, while the optimal TDZ level for wheat regeneration seemed to be 0.9 mM (0.2 mg l Ϫ1 ) (87% for cv. Bob White and 49.4% for cv. Hi-Line). Roots developed normally when the regenerated wheat and barley shoots from TDZ-containing media were transferred to the rooting medium. Comparison with other plant growth regulators commonly used in wheat and barley regeneration media suggested that TDZ was among the best for in vitro regeneration of wheat and barley.

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Thidiazuron promotes in vitro regeneration of wheat and barley

Shan

2000

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

show abstract

“…Plants were successfully regenerated from a callus that resulted from the growth of young wheat embryos (14-31 d old) on RM-64 media supplemented with 2,4-D (10). Plants from regenerable callus, obtained by growing immature embryos on MS' + 2,4-D, have even been reported to produce seeds (9). Again, immature embryos of Medicago sativa directly produced complete plants in hormone-free MS medium (5).…”

mentioning

confidence: 99%

Root Formation in Deteriorated (Aged) Wheat Embryos

Das

Sen-Mandi²

1988

Plant Physiol.

View full text Add to dashboard Cite

Inability of aged seeds to grow into successful plants in the field is primarily due to their age-associated loss of rooting ability. The present work describes an attempt to initiate roots in nonrooting aged embryos of wheat (Triticum aestivum L.). Data presented give a comparative study of root formation and seedling growth on different culture media. Such studies indicate that sucrose alone is enough to bring about root development in nonrooting aged embryos.The ability of nutrient media to achieve improved growth of isolated immature and mature embryos has often been reported in the literature. Excised barley embryos measuring 90 ,um in length could grow rapidly in phosphate-enriched White's medium (7). Plants were successfully regenerated from a callus that resulted from the growth of young wheat embryos (14-31 d old) on RM-64 media supplemented with 2,4-D (10). Plants from regenerable callus, obtained by growing immature embryos on MS' + 2,4-D, have even been reported to produce seeds (9). Again, immature embryos of Medicago sativa directly produced complete plants in hormone-free MS medium (5).Embryos that have completed organogenesis (mature embryos) but have failed to germinate in intact seeds under favorable germination conditions (presumably due to deterioration of inherent germination ability) have been reported to germinate successfully in culture media (3). Growth of mature, nongerminating (due to insufficient pre-germination imbibition) embryos of pea when cultured aseptically in hormone-free medium of Raghavan and Torry (8) was reported to respond to different concentrations of sucrose in the medium (4). Our work describes the effect of nutrient medium on rooting ability and the growth pattern of embryos isolated from appreciably deteriorated wheat seeds. MATERIALS AND METHODSWheat seeds (Triticum aestivum L. cv HD 2189) were obtained from Indian Agricultural Research Institute, New Delhi, India. Deteriorated wheat seeds showing low germination2 percentage (I1% G) were prepared by the method described earlier (2).Dehydrogenase activity was assayed by reducing TTC to form formazan. Hundreds of 99% G and 1% G wheat seeds were imbibed in water for 1 h and then incubated on moist cotton at 'Abbreviations: MS, Murashige and Skoog medium; G, germinating; HMS, half the concentration of all the components of MS medium; TTC, 2,4-triphenyltetrazolium chloride. 2Growth of embryos initiated by protrusion of radicle or plumule.25 ± 1°C for 1 h. The seeds were then cut longitudinally into two identical halves; each half was immersed in freshly prepared 0.2% TTC solution for 20 min at 25 ± 1°C. The half seeds were washed thoroughly in distilled water and were placed in moist cotton, embryo facing upward. The extent of colored region in each half was assessed visually. The stainability pattern of individual embryos was categorized into diagrammatic representations ( Fig. 1, A-F). The percentage of embryos stained under each category was scored in the two G stocks (viz. 99% and 1% G). The results are pres...

show abstract

“…The Gramineae, on the other hand, are known to be difficult to regenerate (1,30). However, major crop species including maize (Zea mays L., 18), oat (Avena sativa L., 10), pearl millet (Pennisetum typhoides [Burm.] Stapf et Hubb,29), proso millet (Panicum miliaceum L., 1 1), sorghum (Sorghum bicolor Moench, 5), barley (Hordeum vulgare L., 2), wheat (Triticum aestivum L., 25), and rice (Oryza sativa L., 12) have been regenerated with varying degrees of success, but certainly not with the ease of the model dicot systems.…”

mentioning

confidence: 99%