Tissue damage in rat ovaries subjected to torsion and detorsion: effects of l-carnitine and N-acetyl cysteine

Abstract

We aimed to evaluate histopathological changes, to detect HIF-1alpha staining intensities and to determine MDA levels in rat ovaries, which were subjected to torsion and detorsion and treated with L -carnitine or N-acetyl cysteine (NAC). Forty-eight prepubertal female Sprague-Dawley rats were divided into five groups (n = 8): 1, control; 2, ischemia; 3, reperfusion; 4, L -carnitine; and 5, NAC groups. In groups 3, 4 and 5, an ischemic period of 3 h was followed by reperfusion for 24 h. In groups 4 and 5, ischemia was performed and either L -carnitine or NAC was infused intraperitoneally 30 min before reperfusion. Ovarian tissues were examined histopathologically; tissue MDA levels and serum IL-6 levels were determined biochemically. HIF-1alpha was applied to all ovaries immunohistochemically. Total tissue damage scores, tissue MDA levels and HIF-1alpha scores, were significantly higher in group 2 (all P < 0.001) than group 4, and group 3 than group 4 (P < 0.001, P = 0.05 and P < 0.001, respectively). They were also significantly higher in group 2 (all P < 0.001) than group 5. When group 3 is compared to group 5, total tissue damage scores and tissue MDA levels were significantly higher in the former (P < 0.01 and P < 0.001, respectively). Serum IL-6 levels were significantly higher in group 2 when compared to groups 1, 4 and 5 (all P < 0.01). The degree of tissue damage of the torsioned ovaries decreased after a reperfusion period of 24 h in the torsioned ovaries. However, ovaries of both L -carnitine and NAC groups showed better recovery than the reperfusion group.

The protective effects of beta-carotene against ischemia/reperfusion injury in rat ovarian tissue

“…Lipid peroxidation is measured by tissue MDA assay as an indirect index of reactive oxygen metabolites and a biochemical marker of tissue I/R injury (Erkasap et al, 2004). In this study, the MDA level increased significantly after I/R induction, as in previous studies (Aragno et al, 2003;Sahin et al, 2008;Sehirli et al, 2003;Usta et al, 2008). Our results showed that beta-carotene administration significantly reduced MDA production.…”

Histone acetyltransferase KAT8 is essential for mouse oocyte development by regulating ROS levels

“…N-acetylcysteine (NAC) is a precursor of intracellular cysteine and reduced glutathione and a proven potent antioxidant in various tissue types (Danilovic et al, 2011;Inci et al, 2007;Usta et al, 2008). Following intraperitoneal injection of 150 mg/kg NAC daily for 7 days, folliculogenesis in WT mice did not differ detectably from that of mice injected with PBS only (WT+ PBS; data not shown).…”

N-acetylcysteine improves function and follicular survival in mice ovarian grafts through inhibition of oxidative stress

“…This causes endothelial injury, increases microvascular permeability and tissue swelling, as well as starting the inflammatory responses through activation of adhesion molecules and enhanced cytokine production (Damous et al, 2009;Khan et al, 2004). The production of ROS is also directly involved in the oxidative damage of cellular macromolecules, such as nucleic acids, proteins and lipids in ischaemic tissues (Usta et al, 2008). All of these events can lead to a massive follicular loss in the transplanted ovarian tissue (Damous et al, 2009;Gao et al, 2013;Hirayama et al, 2011;Kim, 2010;Skaznik-Wikiel et al, 2011).…”

“…N-acetylcysteine (NAC) has been extensively studied as a potent antioxidant in several studies (Danilovic et al, 2011;Inci et al, 2007;Usta et al, 2008). It is an aminothiol, a precursor of intracellular cysteine and reduced glutathione (Inci et al, 2007;Sun, 2010), which easily penetrates the cell membranes (Usta et al, 2008).…”