Tissue distribution and kinetics of dissolved and nanoparticulate silver in Iceland scallop (Chlamys islandica)

Al-Sid-Cheikh, Maya; Rouleau, Claude; Pelletier, Émilien

doi:10.1016/j.marenvres.2013.02.003

Cited by 39 publications

(28 citation statements)

References 33 publications

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“…It has been used to provide an overall picture of the systemic distribution of radiolabeled drugs or NP and even allows for a semiquantitative assessment of NP in tissues [ 61 – 62 ]. Various radioisotopes, including those of silver ( 110m Ag), carbon ( 14 C) or indium ( 111 I) have been used to label NP and to study their distribution throughout the whole body [ 63 – 65 ]. Radiation from these isotopes is usually detected and quantified by a gamma counter, micro imager, phosphoimager plates or autoradiographic films that are commonly used for X-ray exposures.…”

Section: Reviewmentioning

confidence: 99%

Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques

Ostrowski¹,

Nordmeyer²,

Boreham³

et al. 2015

Beilstein J. Nanotechnol.

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SummaryThe increasing interest and recent developments in nanotechnology pose previously unparalleled challenges in understanding the effects of nanoparticles on living tissues. Despite significant progress in in vitro cell and tissue culture technologies, observations on particle distribution and tissue responses in whole organisms are still indispensable. In addition to a thorough understanding of complex tissue responses which is the domain of expert pathologists, the localization of particles at their sites of interaction with living structures is essential to complete the picture. In this review we will describe and compare different imaging techniques for localizing inorganic as well as organic nanoparticles in tissues, cells and subcellular compartments. The visualization techniques include well-established methods, such as standard light, fluorescence, transmission electron and scanning electron microscopy as well as more recent developments, such as light and electron microscopic autoradiography, fluorescence lifetime imaging, spectral imaging and linear unmixing, superresolution structured illumination, Raman microspectroscopy and X-ray microscopy. Importantly, all methodologies described allow for the simultaneous visualization of nanoparticles and evaluation of cell and tissue changes that are of prime interest for toxicopathologic studies. However, the different approaches vary in terms of applicability for specific particles, sensitivity, optical resolution, technical requirements and thus availability, and effects of labeling on particle properties. Specific bottle necks of each technology are discussed in detail. Interpretation of particle localization data from any of these techniques should therefore respect their specific merits and limitations as no single approach combines all desired properties.

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Section: Reviewmentioning

confidence: 99%

Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques

Ostrowski¹,

Nordmeyer²,

Boreham³

et al. 2015

Beilstein J. Nanotechnol.

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“…In bivalves, bioaccumulation of Ag and adverse effects such as alterations in genes related with metal detoxification/metabolism regulation, in antioxidant capacity and in embryo development have been reported after exposure to Ag NPs [9–13, 16–18]. Al-Sid-Cheikh et al [18] found that scallops ( Chlamys islandica ) can accumulate significant quantities of Ag NPs in a short time followed by an efficient depuration process.…”

Section: Introductionmentioning

confidence: 99%

“…Al-Sid-Cheikh et al [18] found that scallops ( Chlamys islandica ) can accumulate significant quantities of Ag NPs in a short time followed by an efficient depuration process. In the mussel Mytilus edulis , exposed to radio-labelled Ag NPs (<40 nm, 0.7 mg/L), 60% accumulated in the soft tissues with maximum concentration in the digestive gland, whilst some 7% was found in the mussel’s extrapallial fluid [16].…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of Toxicity of Ag Nanoparticles in Comparison to Bulk and Ionic Ag on Mussel Hemocytes and Gill Cells

et al. 2015

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Silver nanoparticles (Ag NPs) are increasingly used in many products and are expected to end up in the aquatic environment. Mussels have been proposed as marine model species to evaluate NP toxicity in vitro. The objective of this work was to assess the mechanisms of toxicity of Ag NPs on mussel hemocytes and gill cells, in comparison to ionic and bulk Ag. Firstly, cytotoxicity of commercial and maltose stabilized Ag NPs was screened in parallel with the ionic and bulk forms at a wide range of concentrations in isolated mussel cells using cell viability assays. Toxicity of maltose alone was also tested. LC50 values were calculated and the most toxic Ag NPs tested were selected for a second step where sublethal concentrations of each Ag form were tested using a wide array of mechanistic tests in both cell types. Maltose-stabilized Ag NPs showed size-dependent cytotoxicity, smaller (20 nm) NPs being more toxic than larger (40 and 100 nm) NPs. Maltose alone provoked minor effects on cell viability. Ionic Ag was the most cytotoxic Ag form tested whereas bulk Ag showed similar cytotoxicity to the commercial Ag NPs. Main mechanisms of action of Ag NPs involved oxidative stress and genotoxicity in the two cell types, activation of lysosomal AcP activity, disruption of actin cytoskeleton and stimulation of phagocytosis in hemocytes and increase of MXR transport activity and inhibition of Na-K-ATPase in gill cells. Similar effects were observed after exposure to ionic and bulk Ag in the two cell types, although generally effects were more marked for the ionic form. In conclusion, results suggest that most observed responses were due at least in part to dissolved Ag.

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“…tion of radiolabeled drugs or NP and even allows for a semiquantitative assessment of NP in tissues [61,62]. Various radioisotopes, including those of silver ( 110m Ag), carbon ( 14 C) or indium ( 111 I) have been used to label NP and to study their distribution throughout the whole body [63][64][65]. Radiation from these isotopes is usually detected and quantified by a gamma counter, micro imager, phosphoimager plates or autoradiographic films that are commonly used for X-ray exposures.…”

Section: Light and Electron Microscopic Autoradiographymentioning

confidence: 99%

“…Radiation from these isotopes is usually detected and quantified by a gamma counter, micro imager, phosphoimager plates or autoradiographic films that are commonly used for X-ray exposures. However, such autoradiographs performed on cross sections of whole bodies or total organs of animals (Figure 2a and Figure 2b) [62,63,[66][67][68] possess optical resolution limited to around one millimeter and fail to provide information on the cell or tissue levels [61].…”

Section: Light and Electron Microscopic Autoradiographymentioning

confidence: 99%

Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques

Ostrowski¹,

Nordmeyer²,

Boreham³

et al. 2016

Nano Online

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Tissue distribution and kinetics of dissolved and nanoparticulate silver in Iceland scallop (Chlamys islandica)

Cited by 39 publications

References 33 publications

Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques

Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques

Mechanisms of Toxicity of Ag Nanoparticles in Comparison to Bulk and Ionic Ag on Mussel Hemocytes and Gill Cells

Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques

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