Tissue distribution of <i>Ornithobacterium rhinotracheale</i> in experimentally infected turkeys

Back, Alberto; Rajashekara, Gireesh; Jeremiah, Richard; Halvorson, David A.; Nagaraja, K. V.

doi:10.1136/vr.143.2.52

Cited by 22 publications

(5 citation statements)

References 6 publications

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“…Group (2) infection with ORT alone show nasal discharge, coughing and reduce appetite during first week post-infection, while group (3) broilers infected with ORT and treated with lincospectine 1g/L 5 successive days showed decrease mortality and morbidity. The result are in agreement with other ptrviously cited studies (Hafez et al, 1993 andBack et al, 1996).…”

Section: Results Of Pathogenicity Of Local Isolates Ort To Broiler Ch...supporting

confidence: 93%

Some Studies on Ornithobacterium Rhinotracheale (Ort) in Broiler Chickens

Masoud

El-Banna²,

A.³

et al. 2015

Egyptian Journal of Chemistry and Environmental Health

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Affected 100 flocks revealed chronic whitish diarrhea. Consistent gross pathological lesions recorded were congestion and consolidation of lungs, hemorrhages in trachea, congestion and hemorrhages in liver, splenomegaly and necrotic foci on the kidneys, mortality in various flocks ranged from 3.7 -15% detailed isolation and biochemical studies revealed that Ornithobacterium Rhinotracheale was responsible for this wide spread respiratory problem. Five ORT isolate positive PCR implications were pre elicited to be the size of 784 bpfinally it was confirmed that sequence analysis of 16 sr RNA and ORT isolates from Gen bank with identified from 94% to 98%. Antibacterial sensitivity revealed that most of the isolates were sensitive to lincospectin (lincomycin + spectinomycin) and doxycycline. Many of the isolates showed resistance to kanamycin, norfloxacin, enrofloxacin, tetracycline and chloramphenicol. Experimental infection of broiler orally with ORT evoked respiratory signs with mortality reached 20% lesions of moderated saculitis mild tracheitis and unilateral pneumonia were recorded during the observation period. On other hand of broiler infected with ORT revealed that the infected and treated broiler were lower significance than infected and treated with lincospectin. The microscopical examination revealed pathological changes can be seen in lung, trachea, liver, spleen and brain.

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Section: Results Of Pathogenicity Of Local Isolates Ort To Broiler Ch...supporting

confidence: 93%

Some Studies on Ornithobacterium Rhinotracheale (Ort) in Broiler Chickens

Masoud

El-Banna²,

A.³

et al. 2015

Egyptian Journal of Chemistry and Environmental Health

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“…Both the IT and IV IR of our model allowed the successful study of O. rhinotracheale induced lesions as was noted in previously reported experimental models (van Empel et al, 1996;Sprenger et al, 1998;van Veen et al, 2000b;Eroksuz et al, 2006;Kiliç et al, 2009;Walters et al, 2014), but, importantly, our study achieved the successful experimental inoculation of both BH and NH phenotypes of O. rhinotracheale by two IR in broiler chickens in order to clarify differences in pathogenicity for the first time. Furthermore, our experimental model contrasted with the absence of experimentally induced lesions in turkeys previously reported by Back et al (1998). Our experimental model highlighted the direct association between the inoculation routes and the organs affected.…”

Section: Discussioncontrasting

confidence: 42%

A combined retrospective, prospective and experimental study ofOrnithobacterium rhinotrachealeinfection in chickens

et al. 2019

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The aim of this study was to examine the histopathological and immunohistochemical changes caused by natural and experimentally-induced Ornithobacterium rhinotracheale infection in the respiratory system of chickens. To this end, three different studies were carried out. The first was a retrospective study of 82 field cases with respiratory disorders compatible with O. rhinotracheale infection. The bacterium was immunohistochemically detected in the lungs in 48 of 82 field cases, and 50 β-haemolytic (BH) and non-haemolytic (NH) strains were isolated. In the second study, an experimental model of the disease was created using 3week-old broiler chickens, to identify possible differences of pathogenicity between the BH and NH isolates by the intravenous (IV) and intratracheal (IT) inoculation routes (IR). The group challenged with the NH isolate showed more severe lung lesions than the group challenged with the BH isolate at 7-days postinoculation (p.i.). The 14-day p.i. groups challenged with either the BH or NH isolates by the IT or IV IR had a higher histologic grade of pulmonary and hepatic lesions and a higher total histologic grade of lesions suggesting more severe pathology with longer time of exposure. A direct association between the inoculation routes and the organs affected was shown. Finally, a slaughterhouse study was carried out from October 2014 to May 2015, in which the histologic grade of lesions was significantly higher in immunohistochemically positive for O. rhinotracheale lungs of dead-onarrival chickens.

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“…In broiler flocks, 68% (13/19) had been found positive, and this was higher than the 60% positive flock found in southern Brazil (8) and the 26% found in broiler flocks in Germany (20). The high prevalence of positive broiler flocks may be because of the continuous introduction of the agent from their parent flocks (6,30). The results revealed that age might influence the seroprevalence of ORT in Thailand.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence and Identification of Ornithobacterium rhinotracheale from Broiler and Broiler Breeder Flocks in Thailand

Chansiripornchai

Wanasawaeng²,

Sasipreeyajan

2007

Avian Diseases

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Ornithobacteriosis is an infectious disease of avian species that has been reported in almost all countries around the world, except Thailand. The objectives of this study were to determine the seroprevalence of Ornithobacterium rhinotracheale (ORT) and to isolate and identify ORT in broilers and broiler breeders in Thailand. Chicken antibodies had been randomly checked from 17 farms (19 flocks) of broilers and 23 farms (28 flocks) of broiler breeders. The seropositive flocks were 63% and 100% in broilers and broiler breeders, respectively. The sera analysis showed that the individual 280 broiler sera antibody responses were 67.5% negative, 12.9% suspect, and 19.6% positive. The individual antibody responses of 510 broiler breeder sera revealed 12.2% negative, 38.0% suspect, and 49.8% positive samples. The bacteria were isolated and identified by polymerase chain reaction (PCR). Bacterial isolation and identification revealed that nine isolates of the 12 PCR analysis samples showed positive results to PCR analysis. All the positive PCR samples were collected from the broiler breeder farms.

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Tissue distribution of Ornithobacterium rhinotracheale in experimentally infected turkeys

Cited by 22 publications

References 6 publications

Some Studies on Ornithobacterium Rhinotracheale (Ort) in Broiler Chickens

Some Studies on Ornithobacterium Rhinotracheale (Ort) in Broiler Chickens

A combined retrospective, prospective and experimental study ofOrnithobacterium rhinotrachealeinfection in chickens

Seroprevalence and Identification of Ornithobacterium rhinotracheale from Broiler and Broiler Breeder Flocks in Thailand

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