Tissue engineered buccal mucosa for urethroplasty: Progress and future directions

Osman, Nadir I.; Hillary, Christopher; Bullock, Anthony J.; MacNeil, Sheila; Chapple, Christopher R.

doi:10.1016/j.addr.2014.10.006

Cited by 54 publications

(42 citation statements)

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“…Growing interest in tissue-engineered urethra has spurred the development of various studies and clinical application [7,8] . Due to its complexity of technique and a high recurrence rate [10,11] , tissue-engineered urethra has not been accepted universally in clinical practice.…”

Section: Discussionmentioning

confidence: 99%

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Urethral Reconstruction Using Everted Saphenous Vein Graft in a Rabbit Model: One-Year Outcomes

Xu¹,

Shen

Liu

et al. 2017

Urol Int

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Objective: To investigate the efficacy of using everted saphenous vein graft for urethral reconstruction. Materials and Methods: Thirty-five adult male rabbits were divided into 7 groups randomly: experimental group A, B, C, D, E, stricture control group and normal control group (n = 5). In experimental groups and the stricture control group, a urethral mucosa defect (1.5 × 0.8 cm) was created in each rabbit. In experimental groups, a 2-cm long saphenous vein graft was harvested and incised longitudinally and urethral reconstruction was carried out using the everted saphenous vein patch. Rabbits in experimental group A-E were killed respectively at 1 week, 2 weeks, 1 month, 3 months, and 1 year postoperatively, and the specimens were obtained for histo-pathological examination. Retrograde urethrography was performed to evaluate urethral patency before sacrifice in group D and the stricture control group. Results: In the histo-pathological study, the vein grafts were visible within first week. The vein graft was completely covered by epithelium 1 month postoperatively. Retrograde urethrograms showed the urethral caliber of experimental rabbits were similar to those of normal. While the stricture control group showed a narrow urethral lumen and urothelium defect. Conclusions: For urethral reconstruction, everted saphenous vein graft can be an ideal substitute material because of its longer survival time and rapid epithelization capacity.

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Section: Discussionmentioning

confidence: 99%

“…This technique requires harvesting autologous cells, culturing cells, and seeding them on the biomaterial before urethroplasty. Due to its complex procedure, complicated technology and high recurrence rate, tissue-engineered urethra is not widely utilized in clinical practice [9][10][11] .…”

mentioning

confidence: 99%

Urethral Reconstruction Using Everted Saphenous Vein Graft in a Rabbit Model: One-Year Outcomes

Xu¹,

Shen

Liu

et al. 2017

Urol Int

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“…In this instance, buccal mucosa is taken from the patient and is grown in the laboratory. After a couple of weeks, sheets of tissue-engineered oral mucosa are created 60 . Preliminary data show that tissue-engineered oral mucosa as grafting material has a success rate of 83% 60 .…”

Section: Scar Modulation Tissue Engineering and Future Directionsmentioning

confidence: 99%

Advances in urethral stricture management

Gallegos

Santucci

2016

F1000Res

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Urethral stricture/stenosis is a narrowing of the urethral lumen. These conditions greatly impact the health and quality of life of patients. Management of urethral strictures/stenosis is complex and requires careful evaluation. The treatment options for urethral stricture vary in their success rates. Urethral dilation and internal urethrotomy are the most commonly performed procedures but carry the lowest chance for long-term success (0–9%). Urethroplasty has a much higher chance of success (85–90%) and is considered the gold-standard treatment. The most common urethroplasty techniques are excision and primary anastomosis and graft onlay urethroplasty. Anastomotic urethroplasty and graft urethroplasty have similar long-term success rates, although long-term data have yet to confirm equal efficacy. Anastomotic urethroplasty may have higher rates of sexual dysfunction. Posterior urethral stenosis is typically caused by previous urologic surgery. It is treated endoscopically with radial incisions. The use of mitomycin C may decrease recurrence. An exciting area of research is tissue engineering and scar modulation to augment stricture treatment. These include the use of acellular matrices or tissue-engineered buccal mucosa to produce grafting material for urethroplasty. Other experimental strategies aim to prevent scar formation altogether.

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“…These observations have been taken beyond the tumor environment to virtually all cell types in developing 3D organ-on-a-chip models [57]. Moreover, the use of 3D culture systems allows the identification of more relevant biomarkers [58], an increasingly crucial tool in drug discovery and development. The pathophysiological mechanical environment of the tumor can be mimicked in spheroids.…”

Section: Compressive Forcesmentioning

confidence: 99%

Cellular Biomechanics in Drug Screening and Evaluation: Mechanopharmacology

Krishnan

Park

Seow

et al. 2016

Trends in Pharmacological Sciences

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The study of mechanobiology is now widespread. The impact of cell and tissue mechanics on cellular responses is well appreciated. However, knowledge of the impact of cell and tissue mechanics on pharmacological responsiveness, and its application to drug screening and mechanistic investigations, have been very limited in scope. We emphasize the need for a heightened awareness of the important bidirectional influence of drugs and biomechanics in all living systems. We propose that the term ‘mechanopharmacology’ be applied to approaches that employ in vitro systems, biomechanically appropriate to the relevant (patho)physiology, to identify new drugs and drug targets. This article describes the models and techniques that are being developed to transform drug screening and evaluation, ranging from a 2D environment to the dynamic 3D environment of the target expressed in the disease of interest.

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Tissue engineered buccal mucosa for urethroplasty: Progress and future directions

Cited by 54 publications

References 50 publications

Urethral Reconstruction Using Everted Saphenous Vein Graft in a Rabbit Model: One-Year Outcomes

Urethral Reconstruction Using Everted Saphenous Vein Graft in a Rabbit Model: One-Year Outcomes

Advances in urethral stricture management

Cellular Biomechanics in Drug Screening and Evaluation: Mechanopharmacology

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